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Literature summary extracted from

  • White-Stevens, R.H.; Kamin, H.
    Studies of a flavoprotein, salicylate hydroxylase. I. Preparation, properties, and the uncoupling of oxygen reduction from hydroxylation (1972), J. Biol. Chem., 247, 2358-2370.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.1 benzoate competitive inhibitor Pseudomonas sp.
1.14.13.1 Br-
-
 Pseudomonas sp.
1.14.13.1 Cl-
-
 Pseudomonas sp.
1.14.13.1 F-
-
 Pseudomonas sp.
1.14.13.1 I-
-
 Pseudomonas sp.
1.14.13.1 NO3-
-
 Pseudomonas sp.
1.14.13.1 p-chloromercuribenzoate
-
 Pseudomonas sp.
1.14.13.1 SCN-
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.1 additional information
-
 additional information
-
 Pseudomonas sp.
1.14.13.1 0.0027
-
 salicylate
-
 Pseudomonas sp.
1.14.13.1 0.011
-
 2,6-dihydroxybenzoate
-
 Pseudomonas sp.
1.14.13.1 0.015
-
 p-Aminosalicylate
-
 Pseudomonas sp.
1.14.13.1 0.0167
-
 NADH
-
 Pseudomonas sp.
1.14.13.1 0.017
-
 NADH
-
 Pseudomonas sp.
1.14.13.1 0.028
-
 2,3-Dihydroxybenzoate
-
 Pseudomonas sp.
1.14.13.1 0.065
-
 2,5-Dihydroxybenzoate
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.1 43000 48700 gel filtration, sedimentation equilibrium, calculation of MW per flavin Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.1 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.1
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.1 10.57
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.1 2,3-dihydroxybenzoate + NADH + O2
-
 Pseudomonas sp. pyrogallol + CO2 + H2O + NAD+
-
 ?
1.14.13.1 2,5-dihydroxybenzoate + NADH + O2
-
 Pseudomonas sp. benzene-1,2,5-triol + CO2 + H2O + NAD+
-
 ?
1.14.13.1 2,6-dihydroxybenzoate + NADH + O2
-
 Pseudomonas sp. pyrogallol + CO2 + H2O + NAD+
-
 ?
1.14.13.1 m-hydroxybenzoate + NADH + O2 6% of the reaction with salicylate Pseudomonas sp. 1,3-dihydroxybenzene + CO2 + NAD+ + H2O
-
 ?
1.14.13.1 p-aminosalicylate + NADH + O2
-
 Pseudomonas sp. 1,2-dihydroxy-4-aminobenzene + NAD+ + CO2 + H2O
-
 ?
1.14.13.1 salicylate + NADH + H+ + O2
-
 Pseudomonas sp. catechol + NAD+ + H2O + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.1 dimer 2 * 43000-48700, sedimentation equilibrium Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.1 7 8.5
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.1 FAD flavoprotein Pseudomonas sp.
1.14.13.1 NADH
-
 Pseudomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.13.1 3.1
-
 benzoate
-
 Pseudomonas sp.
1.14.13.1 60
-
 Cl-
-
 Pseudomonas sp.