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Literature summary extracted from

  • Yamamoto, S.; Katagiri, M.; Maeno, H.; Hayaishi, O.
    Salicylate hydroxylase, a monooxygenase requiring flavin adenine dinucleotide (1965), J. Biol. Chem., 240, 3408-3413.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.1 1,10-phenanthroline
-
 Pseudomonas sp.
1.14.13.1 ascorbic acid slight Pseudomonas sp.
1.14.13.1 CuSO4
-
 Pseudomonas sp.
1.14.13.1 Na2MoO4 slight Pseudomonas sp.
1.14.13.1 p-chloromercuribenzoate
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.1 0.0019
-
 salicylate
-
 Pseudomonas sp.
1.14.13.1 0.0026
-
 NADH
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.1 57200
-
 calculation from diffusion and sedimentation data Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.1 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.1
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.1 13.5
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.1 2,3-dihydroxybenzoate + NADH + O2
-
 Pseudomonas sp. pyrogallol + CO2 + H2O + NAD+
-
 ?
1.14.13.1 2,6-dihydroxybenzoate + NADH + O2
-
 Pseudomonas sp. pyrogallol + CO2 + H2O + NAD+
-
 ?
1.14.13.1 salicylate + NADH + H+ + O2
-
 Pseudomonas sp. catechol + NAD+ + H2O + CO2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.1 20
-
 assay at Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.13.1 25
-
 apoenzyme unstable above Pseudomonas sp.
1.14.13.1 38
-
 30 min, complete inactivation in absence of FAD Pseudomonas sp.
1.14.13.1 38
-
 2 h, 10% loss of activity in the presence of FAD Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.1 7.5 8
-
 Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.13.1 6 10 about 50% of activity maximum at pH 6 and 10 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.13.1 8
-
 highest stability Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.1 FAD flavoprotein Pseudomonas sp.
1.14.13.1 FAD 1 mol of FAD loosly bound to 1 mol of enzyme, MW 57000 Pseudomonas sp.
1.14.13.1 NADH
-
 Pseudomonas sp.