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Literature summary extracted from
- Partial purification of L-ascorbate:ferricytochrome b5 oxidoreductase from rat liver microsomes (1977), Hoppe-Seyler's Z. Physiol. Chem., 358, S.1499-1503.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.2.1.3 | asolectin | lipid micelles increases activity about 2fold | Rattus norvegicus | |
| 7.2.1.3 | lecithin | lipid micelles increases activity about 2fold | Rattus norvegicus |  |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 7.2.1.3 | enzymes loses its activity during isoelectric focusing | Rattus norvegicus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.2.1.3 | membrane | - |
Rattus norvegicus | 16020 | - |
| 7.2.1.3 | microsome | - |
Rattus norvegicus | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.2.1.3 | L-(+)-ascorbate + ferricytochrome b5 | Rattus norvegicus | - |
monodehydro-L(+)-ascorbate + ferrocytochrome b5 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.2.1.3 | Rattus norvegicus | - |
rat, Wistar AF/Han | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.2.1.3 | partial | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 7.2.1.3 | liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 7.2.1.3 | 6.5 | - |
microsomes after CM-cellulose-chromatography, ultrafiltration and dialysis P2 | Rattus norvegicus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 7.2.1.3 | 0°C, enzymatic activity tolerates ampholine over a 24 h period | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.2.1.3 | L-(+)-ascorbate + ferricytochrome b5 | - |
Rattus norvegicus | monodehydro-L(+)-ascorbate + ferrocytochrome b5 | - |
? | |
| 7.2.1.3 | L-ascorbate + ferricytochrome b5 | - |
Rattus norvegicus | monodehydro-L-ascorbate + ferrocytochrome b5 | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 7.2.1.3 | 6.9 | - |
enriched enzyme | Rattus norvegicus |
| 7.2.1.3 | 7.2 | 7.4 | in microsomes | Rattus norvegicus |
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