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Literature summary extracted from

  • Matsushita, K.; Shinagawa, E.; Adachi, O.; Ameyama, M.
    Membrane-bound D-gluconate dehydrogenase from Pseudomonas aeruginosa. Its kinetic properties and a reconstitution of gluconate oxidase (1979), J. Biochem., 86, 249-256.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.99.3 2-oxogluconate competitive Pseudomonas aeruginosa
1.1.99.3 o-benzoquinone 0.5 mM, complete inhibition Pseudomonas aeruginosa
1.1.99.3 oxalate mixed-type Pseudomonas aeruginosa
1.1.99.3 oxamate 6.7 mM, 97% inhibition, non-competitive Pseudomonas aeruginosa
1.1.99.3 pyruvate competitive Pseudomonas aeruginosa
1.1.99.3 Quinine hydrochloride 1.0 mM, 55% loss of activity Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.3 0.11
-
 coenzyme Q1
-
 Pseudomonas aeruginosa
1.1.99.3 0.15
-
 phenazine methosulfate
-
 Pseudomonas aeruginosa
1.1.99.3 0.21
-
 2,6-dichlorophenolindophenol
-
 Pseudomonas aeruginosa
1.1.99.3 0.8
-
 D-gluconate pH 6 Pseudomonas aeruginosa
1.1.99.3 2.5
-
 ferricyanide
-
 Pseudomonas aeruginosa
1.1.99.3 3.2
-
 D-gluconate pH 5.0 Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.3 membrane reconstitutive experiments on the enzyme with membrane fraction from which the enzyme has been solubilized and removed Pseudomonas aeruginosa 16020
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.3 Pseudomonas aeruginosa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.3 D-gluconate + 2,6-dichlorophenolindophenol
-
 Pseudomonas aeruginosa 2-oxogluconate + ?
-
 ?
1.1.99.3 D-gluconate + coenzyme Q1
-
 Pseudomonas aeruginosa 2-oxogluconate + ?
-
 ?
1.1.99.3 D-gluconate + ferricyanide
-
 Pseudomonas aeruginosa 2-oxogluconate + ferrocyanide
-
 ?
1.1.99.3 D-gluconate + phenazine methosulfate
-
 Pseudomonas aeruginosa 2-oxogluconate + ?
-
 ?
1.1.99.3 additional information no activity with menadione Pseudomonas aeruginosa ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.3 30
-
-
 Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.99.3 50
-
 5 min, complete inactivation Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.3 5
-
 reaction with ferricyanide Pseudomonas aeruginosa
1.1.99.3 5.5
-
 reaction with coenzyme Q1 Pseudomonas aeruginosa
1.1.99.3 6
-
 phenazine methosulfate Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.99.3 0.07
-
 oxamate pH 5.0 Pseudomonas aeruginosa
1.1.99.3 0.6
-
 oxamate pH 6.0 Pseudomonas aeruginosa
1.1.99.3 2.9
-
 oxalate pH 6.0 Pseudomonas aeruginosa
1.1.99.3 4.6
-
 pyruvate pH 5.0 Pseudomonas aeruginosa
1.1.99.3 17.9
-
 pyruvate pH 6.0 Pseudomonas aeruginosa
1.1.99.3 29
-
 2-oxo-D-gluconate pH 5.0 Pseudomonas aeruginosa
1.1.99.3 123
-
 2-oxo-D-gluconate pH 6.0 Pseudomonas aeruginosa