BRENDA - Enzyme Database show

Purification and characterization of constitutive polyethylene glycol (PEG) dehydrogenase of a PEG 4000-utilizing Flavobacterium sp. No. 203

Yamanaka, H.; Kawai, F.; J. Ferment. Bioeng. 67, 324-330 (1989)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.99.20
pyrroloquinoline quinone
-
Flavobacterium sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.99.20
1,4-benzoquinone
at 0.01-1 mM inhibitor; competitive to 2,6-dichlorophenolindophenol
Flavobacterium sp.
1.1.99.20
arsenite
-
Flavobacterium sp.
1.1.99.20
Cd2+
-
Flavobacterium sp.
1.1.99.20
Co2+
-
Flavobacterium sp.
1.1.99.20
Cu2+
at 1 mM; competitive
Flavobacterium sp.
1.1.99.20
Fe3+
-
Flavobacterium sp.
1.1.99.20
Hg2+
at 1 mM; competitive
Flavobacterium sp.
1.1.99.20
hydrazine
weak
Flavobacterium sp.
1.1.99.20
hydroxylamine
weak
Flavobacterium sp.
1.1.99.20
Mn2+
-
Flavobacterium sp.
1.1.99.20
monoiodoacetate
weak
Flavobacterium sp.
1.1.99.20
Ni2+
-
Flavobacterium sp.
1.1.99.20
p-chloromercuribenzoate
-
Flavobacterium sp.
1.1.99.20
Semicarbazide
weak
Flavobacterium sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.99.20
1
-
PEG-400
-
Flavobacterium sp.
1.1.99.20
1.7
-
PEG-1000
-
Flavobacterium sp.
1.1.99.20
2.8
-
PEG-4000
-
Flavobacterium sp.
1.1.99.20
5.9
-
PEG-6000
-
Flavobacterium sp.
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.99.20
membrane
-
Flavobacterium sp.
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.99.20
57000
-
4 * 57000, Flavobacterium sp. No.203, SDS-PAGE
Flavobacterium sp.
1.1.99.20
220000
-
strain No.203, gel filtration
Flavobacterium sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.99.20
Flavobacterium sp.
-
strains No.202 to 205
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.99.20
strain No. 203, by precipitation with ammonium sulfate, solubilization with benzalkonium chloride, chromatography with DEAE-Toyopearl 650M and hydroxylapatite and gel filtration on Toyopearl HW-55
Flavobacterium sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.99.20
additional information
-
-
Flavobacterium sp.
1.1.99.20
2.59
-
-
Flavobacterium sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.99.20
1-dodecanol + acceptor
-
389908
Flavobacterium sp.
n-dodecanal + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
1-hexanol + acceptor
-
389908
Flavobacterium sp.
n-hexanal + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
1-octanol + acceptor
-
389908
Flavobacterium sp.
n-octanal + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
2-carboxybenzaldehyde + acceptor
-
389908
Flavobacterium sp.
2-carboxybenzoic acid + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
acetaldehyde + acceptor + H2O
-
389908
Flavobacterium sp.
acetic acid + reduced acceptor
-
389908
Flavobacterium sp.
-
1.1.99.20
aliphatic aldehyde + acceptor + H2O
C-3 to C-7
389908
Flavobacterium sp.
carboxylic acid + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
aromatic alcohol + acceptor
-
389908
Flavobacterium sp.
aromatic aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
aromatic aldehyde + acceptor + H2O
-
389908
Flavobacterium sp.
aromatic carboxylic acid + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
benzaldehyde + acceptor + H2O
-
389908
Flavobacterium sp.
benzoic acid + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
benzyl alcohol + acceptor
-
389908
Flavobacterium sp.
benzaldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
beta-phenethyl alcohol + acceptor
-
389908
Flavobacterium sp.
beta-phenethyl aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
cinnamyl alcohol + acceptor
-
389908
Flavobacterium sp.
cinnamyl aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
diethyleneglycol monoethylether
-
389908
Flavobacterium sp.
?
-
-
-
?
1.1.99.20
diols + acceptor
except ethylene glycol, 1,2-and 1,3-propenediol
389908
Flavobacterium sp.
dialdehydes + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
diols + acceptor
diethylene glycol and triethylene glycol are only poor substrates
389908
Flavobacterium sp.
dialdehydes + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
diols + acceptor
overview: with primary alcoholic groups
389908
Flavobacterium sp.
dialdehydes + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
ethylene glycol monoethylether
-
389908
Flavobacterium sp.
?
-
-
-
?
1.1.99.20
ethyleneglycol monophenylether
-
389908
Flavobacterium sp.
?
-
-
-
?
1.1.99.20
ethyleneglycolmono-n-butylether
-
389908
Flavobacterium sp.
?
-
-
-
?
1.1.99.20
additional information
the enzyme shows no activity toward ethylene glycol, glycerol, secondary alcohols and sugar alcohols
389908
Flavobacterium sp.
?
-
-
-
-
1.1.99.20
PEG-1000 + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
1.1.99.20
PEG-200 + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
1.1.99.20
PEG-20000 + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
1.1.99.20
PEG-300 + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
1.1.99.20
PEG-400 + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
1.1.99.20
PEG-4000 + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
1.1.99.20
PEG-6000 + acceptor
most active substrate of all PEGs
389908
Flavobacterium sp.
?
-
-
-
?
1.1.99.20
polyethylene glycol + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
1.1.99.20
primary alcohol + acceptor
C-3 to C-12
389908
Flavobacterium sp.
aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
primary alcohol + acceptor
methanol, glycerol, secondary alcohols and sugar alcohols are no substrates
389908
Flavobacterium sp.
aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
primary alcohol + acceptor
2,6-dichlorophenolindophenol is the acceptor in vitro
389908
Flavobacterium sp.
aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
primary alcohol + acceptor
coenzyme Q1 and Q2 can act as acceptors
389908
Flavobacterium sp.
aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
tetraethylene glycol + acceptor
-
389908
Flavobacterium sp.
tetraethylene glycol aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
trans-cinnamaldehyde + acceptor
-
389908
Flavobacterium sp.
trans-cinnamic acid + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
vanillin aldehyde + acceptor + H2O
-
389908
Flavobacterium sp.
vanillic acid + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
vannilyl alcohol + acceptor
-
389908
Flavobacterium sp.
vannilyl aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.99.20
tetramer
4 * 57000, Flavobacterium sp. No.203, SDS-PAGE
Flavobacterium sp.
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.99.20
40
-
-
Flavobacterium sp.
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.1.99.20
18
50
about half-maximal activity at 18°C and 50°C
Flavobacterium sp.
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.99.20
40
-
stable below
Flavobacterium sp.
1.1.99.20
60
-
78% loss of activity
Flavobacterium sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.99.20
7.5
8
-
Flavobacterium sp.
1.1.99.20
8
-
-
Flavobacterium sp.
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.99.20
6.5
8.5
about 75% of maximal activity at pH 6.5 and pH 8.5
Flavobacterium sp.
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.99.20
7
8
stable
Flavobacterium sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.99.20
2,6-dichlorophenolindophenol
electron acceptor
Flavobacterium sp.
1.1.99.20
coenzyme Q1
electron acceptor
Flavobacterium sp.
1.1.99.20
coenzyme Q2
electron acceptor
Flavobacterium sp.
1.1.99.20
additional information
nitroblue tetrazolium, cytochrome c, ferricyanide, NADP+, NAD+, and coenzyme Q6 and Q10 do not serve as electron acceptors
Flavobacterium sp.
1.1.99.20
pyrroloquinoline quinone
prosthetic group, noncovalently bound to the enzyme
Flavobacterium sp.
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.99.20
pyrroloquinoline quinone
-
Flavobacterium sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.99.20
2,6-dichlorophenolindophenol
electron acceptor
Flavobacterium sp.
1.1.99.20
coenzyme Q1
electron acceptor
Flavobacterium sp.
1.1.99.20
coenzyme Q2
electron acceptor
Flavobacterium sp.
1.1.99.20
additional information
nitroblue tetrazolium, cytochrome c, ferricyanide, NADP+, NAD+, and coenzyme Q6 and Q10 do not serve as electron acceptors
Flavobacterium sp.
1.1.99.20
pyrroloquinoline quinone
prosthetic group, noncovalently bound to the enzyme
Flavobacterium sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.99.20
1,4-benzoquinone
at 0.01-1 mM inhibitor; competitive to 2,6-dichlorophenolindophenol
Flavobacterium sp.
1.1.99.20
arsenite
-
Flavobacterium sp.
1.1.99.20
Cd2+
-
Flavobacterium sp.
1.1.99.20
Co2+
-
Flavobacterium sp.
1.1.99.20
Cu2+
at 1 mM; competitive
Flavobacterium sp.
1.1.99.20
Fe3+
-
Flavobacterium sp.
1.1.99.20
Hg2+
at 1 mM; competitive
Flavobacterium sp.
1.1.99.20
hydrazine
weak
Flavobacterium sp.
1.1.99.20
hydroxylamine
weak
Flavobacterium sp.
1.1.99.20
Mn2+
-
Flavobacterium sp.
1.1.99.20
monoiodoacetate
weak
Flavobacterium sp.
1.1.99.20
Ni2+
-
Flavobacterium sp.
1.1.99.20
p-chloromercuribenzoate
-
Flavobacterium sp.
1.1.99.20
Semicarbazide
weak
Flavobacterium sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.99.20
1
-
PEG-400
-
Flavobacterium sp.
1.1.99.20
1.7
-
PEG-1000
-
Flavobacterium sp.
1.1.99.20
2.8
-
PEG-4000
-
Flavobacterium sp.
1.1.99.20
5.9
-
PEG-6000
-
Flavobacterium sp.
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.99.20
membrane
-
Flavobacterium sp.
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.99.20
57000
-
4 * 57000, Flavobacterium sp. No.203, SDS-PAGE
Flavobacterium sp.
1.1.99.20
220000
-
strain No.203, gel filtration
Flavobacterium sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.99.20
strain No. 203, by precipitation with ammonium sulfate, solubilization with benzalkonium chloride, chromatography with DEAE-Toyopearl 650M and hydroxylapatite and gel filtration on Toyopearl HW-55
Flavobacterium sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.99.20
additional information
-
-
Flavobacterium sp.
1.1.99.20
2.59
-
-
Flavobacterium sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.99.20
1-dodecanol + acceptor
-
389908
Flavobacterium sp.
n-dodecanal + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
1-hexanol + acceptor
-
389908
Flavobacterium sp.
n-hexanal + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
1-octanol + acceptor
-
389908
Flavobacterium sp.
n-octanal + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
2-carboxybenzaldehyde + acceptor
-
389908
Flavobacterium sp.
2-carboxybenzoic acid + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
acetaldehyde + acceptor + H2O
-
389908
Flavobacterium sp.
acetic acid + reduced acceptor
-
389908
Flavobacterium sp.
-
1.1.99.20
aliphatic aldehyde + acceptor + H2O
C-3 to C-7
389908
Flavobacterium sp.
carboxylic acid + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
aromatic alcohol + acceptor
-
389908
Flavobacterium sp.
aromatic aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
aromatic aldehyde + acceptor + H2O
-
389908
Flavobacterium sp.
aromatic carboxylic acid + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
benzaldehyde + acceptor + H2O
-
389908
Flavobacterium sp.
benzoic acid + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
benzyl alcohol + acceptor
-
389908
Flavobacterium sp.
benzaldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
beta-phenethyl alcohol + acceptor
-
389908
Flavobacterium sp.
beta-phenethyl aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
cinnamyl alcohol + acceptor
-
389908
Flavobacterium sp.
cinnamyl aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
diethyleneglycol monoethylether
-
389908
Flavobacterium sp.
?
-
-
-
?
1.1.99.20
diols + acceptor
except ethylene glycol, 1,2-and 1,3-propenediol
389908
Flavobacterium sp.
dialdehydes + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
diols + acceptor
diethylene glycol and triethylene glycol are only poor substrates
389908
Flavobacterium sp.
dialdehydes + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
diols + acceptor
overview: with primary alcoholic groups
389908
Flavobacterium sp.
dialdehydes + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
ethylene glycol monoethylether
-
389908
Flavobacterium sp.
?
-
-
-
?
1.1.99.20
ethyleneglycol monophenylether
-
389908
Flavobacterium sp.
?
-
-
-
?
1.1.99.20
ethyleneglycolmono-n-butylether
-
389908
Flavobacterium sp.
?
-
-
-
?
1.1.99.20
additional information
the enzyme shows no activity toward ethylene glycol, glycerol, secondary alcohols and sugar alcohols
389908
Flavobacterium sp.
?
-
-
-
-
1.1.99.20
PEG-1000 + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
1.1.99.20
PEG-200 + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
1.1.99.20
PEG-20000 + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
1.1.99.20
PEG-300 + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
1.1.99.20
PEG-400 + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
1.1.99.20
PEG-4000 + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
1.1.99.20
PEG-6000 + acceptor
most active substrate of all PEGs
389908
Flavobacterium sp.
?
-
-
-
?
1.1.99.20
polyethylene glycol + acceptor
-
389908
Flavobacterium sp.
?
-
-
-
?
1.1.99.20
primary alcohol + acceptor
C-3 to C-12
389908
Flavobacterium sp.
aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
primary alcohol + acceptor
methanol, glycerol, secondary alcohols and sugar alcohols are no substrates
389908
Flavobacterium sp.
aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
primary alcohol + acceptor
2,6-dichlorophenolindophenol is the acceptor in vitro
389908
Flavobacterium sp.
aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
primary alcohol + acceptor
coenzyme Q1 and Q2 can act as acceptors
389908
Flavobacterium sp.
aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
tetraethylene glycol + acceptor
-
389908
Flavobacterium sp.
tetraethylene glycol aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
trans-cinnamaldehyde + acceptor
-
389908
Flavobacterium sp.
trans-cinnamic acid + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
vanillin aldehyde + acceptor + H2O
-
389908
Flavobacterium sp.
vanillic acid + reduced acceptor
-
389908
Flavobacterium sp.
?
1.1.99.20
vannilyl alcohol + acceptor
-
389908
Flavobacterium sp.
vannilyl aldehyde + reduced acceptor
-
389908
Flavobacterium sp.
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.99.20
tetramer
4 * 57000, Flavobacterium sp. No.203, SDS-PAGE
Flavobacterium sp.
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.99.20
40
-
-
Flavobacterium sp.
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.1.99.20
18
50
about half-maximal activity at 18°C and 50°C
Flavobacterium sp.
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.99.20
40
-
stable below
Flavobacterium sp.
1.1.99.20
60
-
78% loss of activity
Flavobacterium sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.99.20
7.5
8
-
Flavobacterium sp.
1.1.99.20
8
-
-
Flavobacterium sp.
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.99.20
6.5
8.5
about 75% of maximal activity at pH 6.5 and pH 8.5
Flavobacterium sp.
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.99.20
7
8
stable
Flavobacterium sp.