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Literature summary extracted from

  • Hamilton, G.A.; Adolf, P.K.; de Jersey, J.; DuBois, G.C.; Dyrkacz, G.R.; Libby, R.D.
    Trivalent copper, superoxide, and galactose oxidase (1978), J. Am. Chem. Soc., 100, 1899-1912.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.3.9 chloroiridate marked increase of activity Hypomyces rosellus
1.1.3.9 EDTA up to 7fold activation Hypomyces rosellus
1.1.3.9 ferricyanide marked activation at 1 mM Hypomyces rosellus
1.1.3.9 superoxide stimulation of O2 uptake Hypomyces rosellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.9 ferricyanide inhibition at 0.001 mM, activation at 1 mM Hypomyces rosellus
1.1.3.9 H2O2 irreversible inactivation, only during active catalysis Hypomyces rosellus
1.1.3.9 Superoxide dismutase 50% inhibition at 50 pM Hypomyces rosellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.9 extracellular
-
 Hypomyces rosellus
-
-
1.1.3.9 extracellular
-
 Polyporus circinatus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.9 Cu3+ first evidence of oxidation by the Cu(III) enzyme with concomitant formation of a Cu(I) enzyme Hypomyces rosellus
1.1.3.9 Mn2+ marked increase of actvitiy at micromolar concentrations Hypomyces rosellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.9 D-galactosylpyranoside + O2 Hypomyces rosellus
-
 D-galacto-hexodialdose + H2O2
-
 ?
1.1.3.9 D-galactosylpyranoside + O2 Polyporus circinatus
-
 D-galacto-hexodialdose + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.9 Hypomyces rosellus
-
-
-
1.1.3.9 Polyporus circinatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.9 to homogeneity, chromatography techniques Hypomyces rosellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.9 mycelium
-
 Hypomyces rosellus
-
1.1.3.9 mycelium
-
 Polyporus circinatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.9 468
-
-
 Hypomyces rosellus

Storage Stability

EC Number Storage Stability Organism
1.1.3.9 4°C, 0.01 M phosphate buffer, pH 7.0, 0.1 mg/ml protein, 30% decrease of activity in 1 year Hypomyces rosellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.9 1-O-methyl-alpha-D-galactosylpyranoside + O2
-
 Hypomyces rosellus 1-O-methyl-alpha-D-galacto-hexodialdose + H2O2
-
 ?
1.1.3.9 1-O-methyl-alpha-D-galactosylpyranoside + O2
-
 Hypomyces rosellus 1-O-methyl-alpha-D-galacto-hexodialdose + H2O2
-
 ir
1.1.3.9 1-O-methyl-alpha-D-galactosylpyranoside + O2
-
 Polyporus circinatus 1-O-methyl-alpha-D-galacto-hexodialdose + H2O2
-
 ?
1.1.3.9 D-galactopyranose + ferricyanide ferricyanide poorly replaces O2 as electron acceptor Hypomyces rosellus D-galacto-hexodialdose + ferrocyanide
-
 ?
1.1.3.9 D-galactosylpyranoside + O2
-
 Hypomyces rosellus D-galacto-hexodialdose + H2O2
-
 ?
1.1.3.9 D-galactosylpyranoside + O2
-
 Polyporus circinatus D-galacto-hexodialdose + H2O2
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.9 20
-
 1-O-methyl-alpha-D-galactopyranoside
-
 Hypomyces rosellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.9 6 7
-
 Hypomyces rosellus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.9 5.5
-
 slow inactivation below Hypomyces rosellus
1.1.3.9 8
-
 slow inactivation above Hypomyces rosellus