EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.3.4 | cloning of the gene encoding the enzyme and expression in Escherichia coli | Penicillium amagasakiense |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.4 | 5.7 | - |
beta-D-glucose | native enzyme | Penicillium amagasakiense | |
1.1.3.4 | 6.2 | - |
beta-D-glucose | recombinant enzyme | Penicillium amagasakiense | |
1.1.3.4 | 6.3 | - |
beta-D-glucose | deglycosylated enzyme | Penicillium amagasakiense | |
1.1.3.4 | 8.3 | - |
2-deoxy-D-glucose | recombinant enzyme | Penicillium amagasakiense | |
1.1.3.4 | 106 | - |
D-mannose | recombinant enzyme | Penicillium amagasakiense | |
1.1.3.4 | 384 | - |
D-xylose | recombinant enzyme | Penicillium amagasakiense | |
1.1.3.4 | 952 | - |
D-galactose | recombinant enzyme | Penicillium amagasakiense |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.3.4 | 60000 | - |
2 * 60000, SDS-PAGE, recombinant enzyme | Penicillium amagasakiense |
1.1.3.4 | 120000 | - |
recombinant enzyme, native PAGE | Penicillium amagasakiense |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.4 | Penicillium amagasakiense | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.1.3.4 | no glycoprotein | recombinant enzyme is nonglycosylated | Penicillium amagasakiense |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.3.4 | of the reactivated recombinant enzyme, using mild acidification and anion-exchange chromatography on Q-Sepharose | Penicillium amagasakiense |
EC Number | Renatured (Comment) | Organism |
---|---|---|
1.1.3.4 | refolding after treatment with 8 M urea and 30 mM dithiothreitol and subsequent dilution in a buffer containing reduced glutathione, FAD and glycerol | Penicillium amagasakiense |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.3.4 | additional information | - |
- |
Penicillium amagasakiense |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.4 | 2-deoxy-D-glucose + O2 + H2O | recombinant enzyme | Penicillium amagasakiense | 2-deoxy-D-glucono-1,5-lactone + H2O2 | - |
? | |
1.1.3.4 | beta-D-glucose + O2 + H2O | glucose is the primary substrate, recombinant enzyme | Penicillium amagasakiense | D-glucono-1,5-lactone + H2O2 | - |
? | |
1.1.3.4 | D-galactose + O2 + H2O | recombinant enzyme | Penicillium amagasakiense | ? + H2O2 | - |
? | |
1.1.3.4 | D-xylose + O2 + H2O | recombinant enzyme | Penicillium amagasakiense | ? | - |
? | |
1.1.3.4 | mannose + O2 + H2O | recombinant enzyme | Penicillium amagasakiense | ? + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.3.4 | dimer | 2 * 60000, SDS-PAGE, recombinant enzyme | Penicillium amagasakiense |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.3.4 | 28 | 40 | recombinant enzyme | Penicillium amagasakiense |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.4 | 1950 | - |
beta-D-glucose | deglycosylated enzyme | Penicillium amagasakiense | |
1.1.3.4 | 2000 | - |
beta-D-glucose | recombinant enzyme | Penicillium amagasakiense | |
1.1.3.4 | 2000 | - |
beta-D-glucose | native enzyme | Penicillium amagasakiense |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.4 | 5.2 | 6.2 | recombinant enzyme | Penicillium amagasakiense |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.4 | 4.5 | 5.2 | recombinant enzyme, exhibits more than 80% of the maximum activity | Penicillium amagasakiense |
1.1.3.4 | 5 | 7 | recombinant enzyme, more than 90% of the residual activity retained after 72 h incubation at room temperature | Penicillium amagasakiense |
1.1.3.4 | 6.2 | 6.5 | recombinant enzyme, exhibits more than 80% of the maximum activity | Penicillium amagasakiense |
1.1.3.4 | 7 | - |
above, the recombinant enzyme is slightly less stable than native and deglycosylated enzyme | Penicillium amagasakiense |