EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.3.4 | isolation of the gene encoding the enzyme, DNA sequence analysis of the coding region shows 80% identity to the sequence of a enzyme gene previously published | Aspergillus niger |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.4 | 23.7 | - |
beta-D-glucose | BTL wild-type strain enzyme | Aspergillus niger | |
1.1.3.4 | 31.8 | - |
beta-D-glucose | commercially available enzyme | Aspergillus niger |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.3.4 | 75000 | - |
x * 75000, SDS-PAGE | Aspergillus niger |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.4 | Aspergillus niger | - |
wild-type strain denoted BTL | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.3.4 | from BTL wild-type strain using ammonium sulfate precipitation, column chromatography on Q-Sepharose at pH 6 and pH 4.5 and gel filtration on SW-300 | Aspergillus niger |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.3.4 | additional information | - |
- |
Aspergillus niger |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.4 | beta-D-glucose + O2 + H2O | - |
Aspergillus niger | D-glucono-1,5-lactone + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.3.4 | ? | x * 75000, SDS-PAGE | Aspergillus niger |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.3.4 | 40 | - |
at or below both the BTL wild-type strain enzyme and the commercially available enzyme are stable for at least 48 h | Aspergillus niger |
1.1.3.4 | 55 | - |
the commercially available enzyme is more stable than the BTL wild-type strain enzyme | Aspergillus niger |
1.1.3.4 | 65 | - |
the commercially available enzyme is more stable than the BTL wild-type strain enzyme | Aspergillus niger |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.4 | 2.5 | - |
the BTL wild-type strain enzyme is slightly more stable than the commercially available enzyme | Aspergillus niger |
1.1.3.4 | 3 | - |
the BTL wild-type strain enzyme is slightly more stable than the commercially available enzyme | Aspergillus niger |
1.1.3.4 | 4 | - |
both the BTL wild-type strain enzyme and the commercially available enzyme are stable for at least 48 h | Aspergillus niger |
1.1.3.4 | 5 | - |
both the BTL wild-type strain enzyme and the commercially available enzyme are stable for at least 48 h | Aspergillus niger |
1.1.3.4 | 6 | - |
both the BTL wild-type strain enzyme and the commercially available enzyme are stable for at least 48 h | Aspergillus niger |