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Literature summary extracted from

  • Bentley, R.
    Glucose oxidase (1963), The Enzymes, 2nd Ed. (Boyer, P. D. , Lardy, H. , Myrbäck, K. , eds. ), 7, 567-586.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.3.4
-
 Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.4 2-deoxy-D-glucose
-
 Aspergillus niger
1.1.3.4 8-hydroxyquinoline 11% inhibition at 10 mM Penicillium amagasakiense
1.1.3.4 D-arabinose
-
 Aspergillus niger
1.1.3.4 dimedone partial Penicillium amagasakiense
1.1.3.4 hydrazine partial Penicillium amagasakiense
1.1.3.4 hydroxylamine partial Penicillium amagasakiense
1.1.3.4 phenylhydrazine partial Penicillium amagasakiense
1.1.3.4 Semicarbazide 20% inhibition at 10 mM Penicillium amagasakiense
1.1.3.4 Sodium bisulfite partial Penicillium amagasakiense
1.1.3.4 Sodium nitrate 13% inhibition at 10 mM Penicillium amagasakiense

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.4 154000
-
 crystalline enzyme Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.4 beta-D-glucose + O2 + H2O Aspergillus niger
-
 D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 beta-D-glucose + O2 + H2O Penicillium amagasakiense
-
 D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 beta-D-glucose + O2 + H2O Mycoderma aceti
-
 D-glucono-1,5-lactone + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.4 Aspergillus niger
-
-
-
1.1.3.4 Mycoderma aceti
-
-
-
1.1.3.4 Penicillium amagasakiense
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.4
-
 Penicillium amagasakiense

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.3.4 beta-D-glucose + O2 = D-glucono-1,5-lactone + H2O2 stereochemistry Penicillium amagasakiense
a

Storage Stability

EC Number Storage Stability Organism
1.1.3.4 -15°C crystalline suspension, stable for at least 8 years Penicillium amagasakiense
1.1.3.4 0°C, as a solid, stable for at least 2 years Aspergillus niger
1.1.3.4 0°C, as a solid, stable for at least 2 years Penicillium amagasakiense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.4 2-deoxy-D-glucose + O2 + H2O
-
 Aspergillus niger 2-deoxy-D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 2-deoxy-D-glucose + O2 + H2O 25% relative activity to beta-D-glucose, when determined with a commercial preparation of the enzyme at 0.1 M substrate concentration, 12% relative activity to beta-D-glucose, when determined with a commercial preparation of glucose oxidase, containing catalase, at 0.05 M substrate concentration Penicillium amagasakiense 2-deoxy-D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 6-deoxy-6-fluoro-D-glucose + O2 + H2O 3% relative activity to beta-D-glucose, when determined with an unspecified enzyme at 0.5 M substrate concentration Penicillium amagasakiense 6-deoxy-6-fluoro-D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 beta-D-glucose + O2 + H2O
-
 Aspergillus niger D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 beta-D-glucose + O2 + H2O
-
 Penicillium amagasakiense D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 beta-D-glucose + O2 + H2O
-
 Mycoderma aceti D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 beta-D-glucose + O2 + H2O the enzyme can use 2,6-dichlorophenolindophenol as hydrogen acceptor in addition to oxygen, the rate of glucose oxidation in the presence of 2,6-dichlorophenolindophenol is only 3.3% of that in the presence of oxygen Aspergillus niger D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 beta-D-glucose + O2 + H2O the enzyme can use 2,6-dichlorophenolindophenol as hydrogen acceptor in addition to oxygen, the rate of glucose oxidation in the presence of 2,6-dichlorophenolindophenol is only 3.3% of that in the presence of oxygen Penicillium amagasakiense D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 D-glucosone + O2 + H2O 30% relative activity to beta-D-glucose Penicillium amagasakiense ? + H2O2
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.4 40
-
 up to, soluble enzyme Aspergillus niger
1.1.3.4 40
-
 up to, soluble enzyme Penicillium amagasakiense

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.4 5.6
-
-
 Aspergillus niger
1.1.3.4 5.6
-
-
 Penicillium amagasakiense
1.1.3.4 5.6 5.8 crystalline enzyme Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.4 8
-
 unstable above Penicillium amagasakiense

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.4 FAD 2 mol per mol of enzyme Aspergillus niger
1.1.3.4 FAD 2 mol per mol of enzyme Penicillium amagasakiense