EC Number | General Stability | Organism |
---|---|---|
1.1.3.4 | SDS, 1%, 30°C, pH 5.5, stable 30 h incubation | Aspergillus niger |
1.1.3.4 | urea: 7 M, 5 min, activity fully restored | Aspergillus niger |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.4 | 0.18 | - |
O2 | periodate-oxidized enzyme | Aspergillus niger | |
1.1.3.4 | 0.2 | - |
O2 | native enzyme | Aspergillus niger | |
1.1.3.4 | 26 | - |
D-glucose | native enzyme | Aspergillus niger | |
1.1.3.4 | 28 | - |
beta-D-glucose | periodate-oxidized enzyme | Aspergillus niger |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.3.4 | 150000 | 153000 | periodate-oxidized enzyme | Aspergillus niger |
1.1.3.4 | 150000 | 153000 | sedimentation and diffusion data | Aspergillus niger |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.4 | beta-D-glucose + O2 + H2O | Aspergillus niger | - |
D-glucono-1,5-lactone + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.4 | Aspergillus niger | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.1.3.4 | glycoprotein | native enzyme contains 12% carbohydrate by weight, main component: mannose, the carbohydrate moiety plays a role in increasing the stability of the protein moiety, but does not directly participate in the catalytic activity, in the immunological reactivity, or in maintaining the conformation of the enzyme protein, periodate treatment decreases the carbohydrate content to about 40% of its original value | Aspergillus niger |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.3.4 | commercial preparation | - |
Aspergillus niger | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.4 | beta-D-glucose + O2 + H2O | - |
Aspergillus niger | D-glucono-1,5-lactone + H2O2 | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.3.4 | 72.4 | - |
denaturation point of native enzyme | Aspergillus niger |
1.1.3.4 | 72.8 | - |
denaturation point of periodate-oxidized enzyme | Aspergillus niger |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.4 | 323 | - |
beta-D-glucose | periodate-oxidized enzyme | Aspergillus niger | |
1.1.3.4 | 337 | - |
beta-D-glucose | native enzyme | Aspergillus niger |