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Literature summary extracted from
- Preparation, characterization, and potential application of an immobilized glucose oxidase (1987), Appl. Biochem. Biotechnol., 14, 37-47.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.1.3.4 | analysis | immobilized enzyme on polyacrylamide employed for the determination of glucose concentration in blood sera | Aspergillus niger |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.3.4 | 33 | - |
beta-D-glucose | soluble enzyme | Aspergillus niger |  |
| 1.1.3.4 | 44 | - |
beta-D-glucose | immobilized enzyme | Aspergillus niger | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.3.4 | beta-D-glucose + O2 + H2O | Aspergillus niger | - |
D-glucono-1,5-lactone + H2O2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.3.4 | Aspergillus niger | - |
- |
- |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.3.4 | 4°C, immobilized, 11% loss of activity, 1 year | Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.3.4 | beta-D-glucose + O2 + H2O | - |
Aspergillus niger | D-glucono-1,5-lactone + H2O2 | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.4 | 40 | - |
immobilized on polyacrylamide | Aspergillus niger |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.4 | 40 | - |
up to, soluble enzyme | Aspergillus niger |
| 1.1.3.4 | 50 | - |
up to, immobilized enzyme | Aspergillus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.4 | 5.5 | - |
immobilized on polyacrylamide | Aspergillus niger |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.4 | 5.5 | - |
50°C, most stable, immobilized enzyme | Aspergillus niger |
| 1.1.3.4 | 6 | - |
50°C, most stable at, soluble enzyme | Aspergillus niger |
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