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Literature summary extracted from

  • Rogalski, J.; Fiedurek, J.; Szczordrak, J.; Kapusta, K.; Leonowicz, A.
    Optimization of glucose oxidase synthesis in submerged cultures of Aspergillus niger G-13 mutant (1988), Enzyme Microb. Technol., 10, 508-511.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.4 beta-D-glucose + O2 + H2O Aspergillus niger
-
 D-glucono-1,5-lactone + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.4 Aspergillus niger
-
 G-13 mutant, culture conditions
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.4 partial Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.4 3.1
-
 in the culture filtrate Aspergillus niger
1.1.3.4 6
-
 after isolation from the fermenter liquid by gel filtration, lyophilized preparate Aspergillus niger

Storage Stability

EC Number Storage Stability Organism
1.1.3.4 4°C, lyophilized enzyme, no loss of activity after 1 year, 10% loss of activity after 2 years Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.4 2-deoxy-D-glucose + O2 + H2O 30% relative activity to beta-D-glucose Aspergillus niger 2-deoxy-D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 beta-D-glucose + O2 + H2O
-
 Aspergillus niger D-glucono-1,5-lactone + H2O2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.4 45
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.4 40
-
 60 min Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.4 5.6
-
-
 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.4 4
-
 rapid inactivation below Aspergillus niger
1.1.3.4 6 8
-
 Aspergillus niger