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Literature summary extracted from
- Some properties of cellobiose oxidase from the white-rot fungus Sporotrichum pulverulentum (1985), Biochem. J., 228, 557-564.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.18 | cellobiose | strong substrate inhibition at concentrations higher than 0.015 mM | Phanerodontia chrysosporium |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.18 | 0.012 | - |
2,6-dichlorophenolindophenol | in presence of 0.24 mM O2 | Phanerodontia chrysosporium | |
| 1.1.99.18 | 0.017 | - |
2,6-dichlorophenolindophenol | in absence of O2 | Phanerodontia chrysosporium | |
| 1.1.99.18 | 0.042 | - |
cellobiose | - |
Phanerodontia chrysosporium | |
| 1.1.99.18 | 0.58 | - |
lactose | - |
Phanerodontia chrysosporium | |
| 1.1.99.18 | 3 | - |
benzyl viologen | - |
Phanerodontia chrysosporium | |
| 1.1.99.18 | 6.6 | - |
maltose | - |
Phanerodontia chrysosporium | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.99.18 | extracellular | - |
Phanerodontia chrysosporium | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 74400 | - |
sedimentation equilibrium centrifugation | Phanerodontia chrysosporium |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.99.18 | Phanerodontia chrysosporium | - |
white-rot fungus | - |
| 1.1.99.18 | Phanerodontia chrysosporium | - |
identical with Sporotrichum pulverulentum, 3 isoenzymes | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 1.1.99.18 | glycoprotein | carbohydrate content: 11.9% | Phanerodontia chrysosporium |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.99.18 | 70fold purification | Phanerodontia chrysosporium |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | additional information | - |
- |
Phanerodontia chrysosporium |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.99.18 | 4°C, 50 mM potassium phosphate buffer, pH 6, several months, stable, slowly loss of activity | Phanerodontia chrysosporium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.18 | agarose + O2 | immediate reduction of enzyme | Phanerodontia chrysosporium | ? | - |
? | |
| 1.1.99.18 | cellobiose + benzyl viologen | - |
Phanerodontia chrysosporium | cellobiono-1,5-lactone + reduced benzyl viologen | - |
r | |
| 1.1.99.18 | cellobiose + O2 | - |
Phanerodontia chrysosporium | cellobiono-1,5-lactone + H2O2 | superoxide anion as primary reduced oxygen product, H2O2 is possibly formed by dismutation of superoxide anion | ? | |
| 1.1.99.18 | cellulose + O2 | immediate reduction of enzyme | Phanerodontia chrysosporium | ? | - |
? | |
| 1.1.99.18 | chitin + O2 | immediate reduction of enzyme | Phanerodontia chrysosporium | ? | - |
? | |
| 1.1.99.18 | lactose + O2 | - |
Phanerodontia chrysosporium | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + H2O2 | - |
? | |
| 1.1.99.18 | maltose + O2 | - |
Phanerodontia chrysosporium | 4-O-beta-glucosyl-glucono-1,5-lactone + H2O2 | - |
? | |
| 1.1.99.18 | additional information | substrates: disaccharides, some insoluble polysaccharides, but no monosaccharides, oxygen as electron acceptor, artificial electron acceptors: 2,6-dichlorophenolindophenol, potassium ferricyanide, benzyl viologen, not with: FMN, FAD, riboflavin | Phanerodontia chrysosporium | ? | - |
? | |
| 1.1.99.18 | xylan + O2 | immediate reduction of enzyme | Phanerodontia chrysosporium | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.99.18 | monomer | - |
Phanerodontia chrysosporium |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 2 | 11 | pH 2: below large loss of activity, pH 11: above large loss of activity | Phanerodontia chrysosporium |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.18 | cytochrome b | as prosthetic group | Phanerodontia chrysosporium | |
| 1.1.99.18 | FAD | as prosthetic group | Phanerodontia chrysosporium | |
| 1.1.99.18 | flavin | flavoprotein, FAD as flavin component | Phanerodontia chrysosporium | |
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