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Literature summary extracted from

  • Dickinson, F.M.; Wadforth, C.
    Purification and some properties of alcohol oxidase from alkane-grown Candida tropicalis (1992), Biochem. J., 282, 325-331.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.3.20 Detergents e.g. cholate, activation, purified preparations do not need detergents for activation Candida tropicalis

General Stability

EC Number General Stability Organism
1.1.3.20 CHAPS, 0.5%, stabilizes during solubilization Candida tropicalis
1.1.3.20 cholate, 1%, solubilizes and stabilizes Candida tropicalis
1.1.3.20 phenylmethylsulfonyl fluoride stabilizes during purification and storage Candida tropicalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.20 light complete inactivation, t1/2: 7 min; photochemical inactivation at wavelength of 450 nm, i.e. blue-light region of the spectrum of visible light Candida tropicalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.20 0.0015
-
Tetradecanol
-
Candida tropicalis
1.1.3.20 0.005
-
hexadecanol
-
Candida tropicalis
1.1.3.20 0.0061
-
Dodecanol
-
Candida tropicalis
1.1.3.20 0.042
-
Decanol
-
Candida tropicalis
1.1.3.20 0.061
-
O2
-
Candida tropicalis
1.1.3.20 1
-
Octanol
-
Candida tropicalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.20 membrane
-
Candida tropicalis 16020
-
1.1.3.20 microsome
-
Candida tropicalis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.20 70000
-
2 * 70000, SDS-PAGE Candida tropicalis
1.1.3.20 145000
-
gel filtration Candida tropicalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.20 long-chain alcohol + O2 Candida tropicalis
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.20 Candida tropicalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.20
-
Candida tropicalis

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.3.20 a long-chain alcohol + O2 = a long-chain aldehyde + H2O2 ping-pong mechanism Candida tropicalis
1.1.3.20 a long-chain alcohol + O2 = a long-chain aldehyde + H2O2 2 long-chain alcohol + O2 = 2 long-chain aldehyde + 2 H2O2 Candida tropicalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.20 30
-
-
Candida tropicalis

Storage Stability

EC Number Storage Stability Organism
1.1.3.20 0°C, in the dark stable for at least 10 h Candida tropicalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.20 1-tetradecanol + O2 75% the rate of dodecanol oxidation Candida tropicalis tetradecanal + H2O2
-
?
1.1.3.20 12-hydroxydodecanoic acid + O2 poor substrate Candida tropicalis ?
-
?
1.1.3.20 2-dodecanol + O2
-
Candida tropicalis ?
-
?
1.1.3.20 alpha,omega-alkanediols + O2 from C10 to C16 Candida tropicalis ?
-
?
1.1.3.20 decanol + O2 oxidation at 70% the rate of dodecanol oxidation Candida tropicalis decanal + H2O2
-
?
1.1.3.20 dodecanol + O2 i.e. lauryl alcohol, preferred substrate Candida tropicalis dodecanal + H2O2
-
?
1.1.3.20 hexadecanol + O2 19% the rate of dodecanol oxidation Candida tropicalis hexadecanal + H2O2
-
?
1.1.3.20 long-chain alcohol + O2
-
Candida tropicalis ?
-
?
1.1.3.20 additional information weak activity towards fatty aldehydes Candida tropicalis ?
-
?
1.1.3.20 additional information no substrate: dodecan-4-ol, dodecan-5-ol Candida tropicalis ?
-
?
1.1.3.20 n-octanol + O2 oxidation at 34% the rate of dodecanol oxidation Candida tropicalis n-octanal + H2O2
-
?
1.1.3.20 octadecanol + O2 oxidation at 12% the rate of dodecanol oxidation Candida tropicalis octadecanal + H2O2
-
?

Subunits

EC Number Subunits Comment Organism
1.1.3.20 dimer 2 * 70000, SDS-PAGE Candida tropicalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.20 25
-
inactivation overnight Candida tropicalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.20 9
-
60% activity at pH 7.0 Candida tropicalis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.20 6
-
inactivation after 3 min Candida tropicalis
1.1.3.20 7
-
and below unstable Candida tropicalis
1.1.3.20 9
-
at least 15 min stable Candida tropicalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.20 flavin not FAD, FMN, riboflavin, different absorption and fluorescence emission spectra, flavin is probably covalently bound Candida tropicalis
1.1.3.20 additional information no cofactor: riboflavin, 2,6-dichlorophenolindophenol/phenazine methosulfate, potassium ferricyanide, cytochrome c, nitro blue tetrazolium, tetramethyl-p-phenylenediamine Candida tropicalis