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Literature summary extracted from

  • Bhat, S.G.; Vaidyanathan, C.S.
    Purification and properties of L-4-hydroxymandelate oxidase from Pseudomonas convexa (1976), Eur. J. Biochem., 68, 323-331.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.3.19 after solubilization the enzyme gradually loses activity when kept at 5°C, full reactivation by freezing and thawing Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.19 2,4-Dinitrophenylhydrazine
-
 Pseudomonas putida
1.1.3.19 2-mercaptoethanol
-
 Pseudomonas putida
1.1.3.19 8-hydroxyquinoline
-
 Pseudomonas putida
1.1.3.19 Ag+
-
 Pseudomonas putida
1.1.3.19 Atebrin
-
 Pseudomonas putida
1.1.3.19 Co2+
-
 Pseudomonas putida
1.1.3.19 Cu2+
-
 Pseudomonas putida
1.1.3.19 cysteine
-
 Pseudomonas putida
1.1.3.19 diethyldithiocarbamate
-
 Pseudomonas putida
1.1.3.19 dithiothreitol
-
 Pseudomonas putida
1.1.3.19 DL-3,4-dihydroxymandelic acid
-
 Pseudomonas putida
1.1.3.19 EDTA
-
 Pseudomonas putida
1.1.3.19 Fe2+
-
 Pseudomonas putida
1.1.3.19 Fe3+
-
 Pseudomonas putida
1.1.3.19 guanidine hydrochloride
-
 Pseudomonas putida
1.1.3.19 Hg2+
-
 Pseudomonas putida
1.1.3.19 hydroxylamine
-
 Pseudomonas putida
1.1.3.19 potassium cyanide
-
 Pseudomonas putida
1.1.3.19 reduced glutathione
-
 Pseudomonas putida
1.1.3.19 SDS
-
 Pseudomonas putida
1.1.3.19 Zn2+
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.19 0.44
-
 DL-4-hydroxymandelate
-
 Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.19 membrane bound Pseudomonas putida 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.19 Mn2+ required Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.19 155000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.19 (S)-2-hydroxy-2-(4-hydroxyphenyl)acetate + O2 Pseudomonas putida enzyme of degradation pathway from mandelic acid to benzoic acid 4-hydroxybenzaldehyde + CO2 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.19 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.19 partial Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.19 additional information
-
-
 Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.1.3.19 -20°C, 2 months, solubilized enzyme Pseudomonas putida
1.1.3.19 -20°C, particulate fraction suspended in 0.025 M sodium phosphate buffer, pH 7.0, more than 6 months Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.19 (S)-2-hydroxy-2-(4-hydroxyphenyl)acetate + O2 absolute requirement for molecular oxygen, specific for L-isomer of 4-hydroxymandelic acid Pseudomonas putida 4-hydroxybenzaldehyde + CO2 + H2O2
-
 ?
1.1.3.19 (S)-2-hydroxy-2-(4-hydroxyphenyl)acetate + O2 enzyme of degradation pathway from mandelic acid to benzoic acid Pseudomonas putida 4-hydroxybenzaldehyde + CO2 + H2O2
-
 ?
1.1.3.19 DL-4-hydroxymandelate + ?
-
 Pseudomonas putida ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.19 L-4-hydroxymandelate oxidase (decarboxylating)
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.19 55
-
-
 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.19 6.6
-
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.19 FAD required Pseudomonas putida