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Literature summary extracted from

  • Koolman, J.; Karlson, P.
    Ecdysone oxidase: reaction and specificity (1978), Eur. J. Biochem., 89, 453-460.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.3.16 partial loss of activity by lyophilization Calliphora vicina
1.1.3.16 stable to precipitation with ammonium sulfate and redissolvation or dialysis Calliphora vicina

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.16 HgCl2 above 0.001 mM Calliphora vicina
1.1.3.16 methanol 31% inhibition at 0.5%, 62% inhibition at 10%, 90% inhibition at 20% Calliphora vicina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.16 0.031
-
 20-hydroxyecdysone
-
 Calliphora vicina
1.1.3.16 0.098
-
 ecdysone
-
 Calliphora vicina

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.16 Calliphora vicina
-
-
-

Storage Stability

EC Number Storage Stability Organism
1.1.3.16 -25°C, more than 3 months Calliphora vicina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.16 2-deoxy-20-hydroxyecdysone + O2
-
 Calliphora vicina 3-dehydro-2-deoxy-20-hydroxyecdysone + H2O2
-
 ?
1.1.3.16 2-deoxyecdysone + O2
-
 Calliphora vicina 3-dehydro-2-deoxyecdysone + H2O2
-
 ?
1.1.3.16 20-hydroxyecdysone + O2
-
 Calliphora vicina 3-dehydro-20-hydroxyecdysone + H2O2
-
 ?
1.1.3.16 5,20-dihydroxyecdysone + O2
-
 Calliphora vicina 3-dehydro-5,20-dihydroxyecdysone + H2O2
-
 ?
1.1.3.16 cyasterone + O2
-
 Calliphora vicina 3-dehydrocyasterone + H2O2
-
 ?
1.1.3.16 ecdysone + O2
-
 Calliphora vicina 3-dehydroecdysone + H2O2
-
 ir
1.1.3.16 ecdysteroids + O2
-
 Calliphora vicina 3-dehydroecdysteroids + H2O2
-
 ?
1.1.3.16 inokosterone + O2
-
 Calliphora vicina 3-dehydroinokosterone + H2O2
-
 ?
1.1.3.16 makisterone + O2
-
 Calliphora vicina 3-dehydromakisterone + H2O2
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.16 35
-
 denaturation begins above 35°C Calliphora vicina