BRENDA - Enzyme Database

Effect of anions on the catalytic activity of pig liver glycolic acid oxidase

Schuman, M.; Massey, V.; Biochim. Biophys. Acta 227, 521-537 (1971)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.3.15
arsenate
increasing activity up to 0.1 M, inhibition of ferricyanide-linked assay above 0.1 M
Sus scrofa
1.1.3.15
phosphate
increasing activity up to 0.1 M, inhibition of ferricyanide-linked assay above 0.1 M
Sus scrofa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.3.15
acetate
-
Sus scrofa
1.1.3.15
arsenate
inhibition of glycolate-ferricyanide or glyoxylate-ferricyanide assay above 0.1 M
Sus scrofa
1.1.3.15
Butyrate
-
Sus scrofa
1.1.3.15
glycolate
inhibition above 1.7 mM
Sus scrofa
1.1.3.15
Heptanoate
-
Sus scrofa
1.1.3.15
hexanoate
-
Sus scrofa
1.1.3.15
malonate
-
Sus scrofa
1.1.3.15
oxalate
-
Sus scrofa
1.1.3.15
phosphate
inhibition of glycolate-ferricyanide or glyoxylate-ferricyanide assay above 0.1 M
Sus scrofa
1.1.3.15
propionate
-
Sus scrofa
1.1.3.15
succinate
-
Sus scrofa
1.1.3.15
valerate
-
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.3.15
0.28
-
dichlorophenolindophenol
cosubstrate: glycolate
Sus scrofa
1.1.3.15
0.42
-
glycolate
cosubstrate: 2,6-dichlorophenolindophenol
Sus scrofa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.3.15
glycolate + O2
Sus scrofa
-
glyoxylate + H2O2
-
Sus scrofa
?
1.1.3.15
glyoxylate + O2
Sus scrofa
-
? + H2O2
-
Sus scrofa
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.3.15
Sus scrofa
-
pig
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.3.15
liver
-
Sus scrofa
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.3.15
glycolate + 2,6-dichlorophenolindophenol
-
389695
Sus scrofa
glyoxylate + reduced 2,6-dichlorophenolindophenol
-
389695
Sus scrofa
?
1.1.3.15
glycolate + ferricyanide
-
389695
Sus scrofa
glyoxylate + ferrocyanide
-
389695
Sus scrofa
?
1.1.3.15
glycolate + O2
-
389695
Sus scrofa
glyoxylate + H2O2
-
389695
Sus scrofa
?
1.1.3.15
glyoxylate + 2,6-dichlorophenolindophenol
-
389695
Sus scrofa
? + reduced 2,6-dichlorophenolindophenol
-
389695
Sus scrofa
?
1.1.3.15
glyoxylate + ferricyanide
-
389695
Sus scrofa
? + ferrocyanide
-
389695
Sus scrofa
?
1.1.3.15
glyoxylate + O2
-
389695
Sus scrofa
? + H2O2
-
389695
Sus scrofa
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.3.15
FMN
-
Sus scrofa
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.3.15
additional information
-
additional information
straight-chain monocarboxylic acids are non-competitive inhibitors with either glycolate or glyoxylate as substrates, dicarboxylic acids are competitive inhibitors with glycolate as substrate, but non-competitive inhibitors for glyoxylate as substrate
Sus scrofa
1.1.3.15
0.092
-
Heptanoate
non-competitive, glycolate oxidation
Sus scrofa
1.1.3.15
0.3
-
hexanoate
non-competitive, glycolate oxidation
Sus scrofa
1.1.3.15
0.44
-
oxalate
competitive, glycolate oxidation, non-competitive for glyoxylate oxidation
Sus scrofa
1.1.3.15
1.2
-
valerate
non-competitive, glyoxylate reduction
Sus scrofa
1.1.3.15
1.3
-
valerate
non-competitive, glycolate oxidation
Sus scrofa
1.1.3.15
3.6
-
Butyrate
non-competitive, glycolate oxidation
Sus scrofa
1.1.3.15
8.2
-
malonate
non-competitive, glyoxylate reduction
Sus scrofa
1.1.3.15
9.2
-
malonate
competitive, glycolate oxidation
Sus scrofa
1.1.3.15
9.5
-
propionate
non-competitive, glycolate oxidation
Sus scrofa
1.1.3.15
14
-
succinate
competitive, glycolate oxidation
Sus scrofa
1.1.3.15
17
-
propionate
non-competitive, glyoxylate reduction
Sus scrofa
1.1.3.15
37
-
acetate
non-competitive, glycolate oxidation
Sus scrofa
1.1.3.15
48
-
acetate
non-competitive, glyoxylate reduction
Sus scrofa
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.3.15
arsenate
increasing activity up to 0.1 M, inhibition of ferricyanide-linked assay above 0.1 M
Sus scrofa
1.1.3.15
phosphate
increasing activity up to 0.1 M, inhibition of ferricyanide-linked assay above 0.1 M
Sus scrofa
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.3.15
FMN
-
Sus scrofa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.3.15
acetate
-
Sus scrofa
1.1.3.15
arsenate
inhibition of glycolate-ferricyanide or glyoxylate-ferricyanide assay above 0.1 M
Sus scrofa
1.1.3.15
Butyrate
-
Sus scrofa
1.1.3.15
glycolate
inhibition above 1.7 mM
Sus scrofa
1.1.3.15
Heptanoate
-
Sus scrofa
1.1.3.15
hexanoate
-
Sus scrofa
1.1.3.15
malonate
-
Sus scrofa
1.1.3.15
oxalate
-
Sus scrofa
1.1.3.15
phosphate
inhibition of glycolate-ferricyanide or glyoxylate-ferricyanide assay above 0.1 M
Sus scrofa
1.1.3.15
propionate
-
Sus scrofa
1.1.3.15
succinate
-
Sus scrofa
1.1.3.15
valerate
-
Sus scrofa
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.3.15
additional information
-
additional information
straight-chain monocarboxylic acids are non-competitive inhibitors with either glycolate or glyoxylate as substrates, dicarboxylic acids are competitive inhibitors with glycolate as substrate, but non-competitive inhibitors for glyoxylate as substrate
Sus scrofa
1.1.3.15
0.092
-
Heptanoate
non-competitive, glycolate oxidation
Sus scrofa
1.1.3.15
0.3
-
hexanoate
non-competitive, glycolate oxidation
Sus scrofa
1.1.3.15
0.44
-
oxalate
competitive, glycolate oxidation, non-competitive for glyoxylate oxidation
Sus scrofa
1.1.3.15
1.2
-
valerate
non-competitive, glyoxylate reduction
Sus scrofa
1.1.3.15
1.3
-
valerate
non-competitive, glycolate oxidation
Sus scrofa
1.1.3.15
3.6
-
Butyrate
non-competitive, glycolate oxidation
Sus scrofa
1.1.3.15
8.2
-
malonate
non-competitive, glyoxylate reduction
Sus scrofa
1.1.3.15
9.2
-
malonate
competitive, glycolate oxidation
Sus scrofa
1.1.3.15
9.5
-
propionate
non-competitive, glycolate oxidation
Sus scrofa
1.1.3.15
14
-
succinate
competitive, glycolate oxidation
Sus scrofa
1.1.3.15
17
-
propionate
non-competitive, glyoxylate reduction
Sus scrofa
1.1.3.15
37
-
acetate
non-competitive, glycolate oxidation
Sus scrofa
1.1.3.15
48
-
acetate
non-competitive, glyoxylate reduction
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.3.15
0.28
-
dichlorophenolindophenol
cosubstrate: glycolate
Sus scrofa
1.1.3.15
0.42
-
glycolate
cosubstrate: 2,6-dichlorophenolindophenol
Sus scrofa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.3.15
glycolate + O2
Sus scrofa
-
glyoxylate + H2O2
-
Sus scrofa
?
1.1.3.15
glyoxylate + O2
Sus scrofa
-
? + H2O2
-
Sus scrofa
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.3.15
liver
-
Sus scrofa
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.3.15
glycolate + 2,6-dichlorophenolindophenol
-
389695
Sus scrofa
glyoxylate + reduced 2,6-dichlorophenolindophenol
-
389695
Sus scrofa
?
1.1.3.15
glycolate + ferricyanide
-
389695
Sus scrofa
glyoxylate + ferrocyanide
-
389695
Sus scrofa
?
1.1.3.15
glycolate + O2
-
389695
Sus scrofa
glyoxylate + H2O2
-
389695
Sus scrofa
?
1.1.3.15
glyoxylate + 2,6-dichlorophenolindophenol
-
389695
Sus scrofa
? + reduced 2,6-dichlorophenolindophenol
-
389695
Sus scrofa
?
1.1.3.15
glyoxylate + ferricyanide
-
389695
Sus scrofa
? + ferrocyanide
-
389695
Sus scrofa
?
1.1.3.15
glyoxylate + O2
-
389695
Sus scrofa
? + H2O2
-
389695
Sus scrofa
?