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Literature summary extracted from

  • Schaefer, A.; Bieg, S.; Huwig, A.; Kohring, G.W.; Giffhorn, F.
    Purification by immunoaffinity chromatography, characterization, and structural analysis of a thermostable pyranose oxidase from the white rot fungus Phlebiopsis gigantea (1996), Appl. Environ. Microbiol., 62, 2586-2592.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.10 N-bromosuccinimide completely inhibitory at 1 mM Phlebiopsis gigantea
1.1.3.10 p-chloromercuribenzoate completely inhibits at 1 mM Phlebiopsis gigantea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.10 1.2
-
 D-glucose
-
 Phlebiopsis gigantea
1.1.3.10 16.5
-
 L-sorbose
-
 Phlebiopsis gigantea
1.1.3.10 22.2
-
 D-xylose
-
 Phlebiopsis gigantea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.10 70000
-
 2 * 70000 + 2 * 77000, SDS-PAGE, electron microscopy Phlebiopsis gigantea
1.1.3.10 77000
-
 2 * 70000 + 2 * 77000, SDS-PAGE, electron microscopy Phlebiopsis gigantea
1.1.3.10 273700
-
 calculation on the basis of the Stoke's radius and sedimentation coefficient Phlebiopsis gigantea
1.1.3.10 280000
-
 gel filtration Phlebiopsis gigantea
1.1.3.10 305000
-
 native gradient gel electrophoresis Phlebiopsis gigantea
1.1.3.10 323600
-
 electron microscopy Phlebiopsis gigantea

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.10 Phlebiopsis gigantea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.10 using heat treatment, immunoaffinity chromatography and gel filtration on Superdex 200 Phlebiopsis gigantea

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.10 8.15
-
 purified enzyme Phlebiopsis gigantea

Storage Stability

EC Number Storage Stability Organism
1.1.3.10 40°C, 10 mM sodium phosphate, pH 6-8, half life of enzyme activity: more than 300 days Phlebiopsis gigantea
1.1.3.10 50°C, 10 mM sodium phosphate, pH 7, half life of enzyme activity: more than 110 days Phlebiopsis gigantea
1.1.3.10 65°C, 10 mM sodium phosphate, pH 7, half life of enzyme activity: 1 h Phlebiopsis gigantea
1.1.3.10 inactivated by freezing and thawing Phlebiopsis gigantea
1.1.3.10 room temperature and 30°C, pH 5-10, half life of enzyme activity: more than 300 days Phlebiopsis gigantea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.10 D-galactose + O2 8.5% relative activity to D-glucose Phlebiopsis gigantea 2-dehydro-D-galactose + H2O2
-
 ?
1.1.3.10 D-glucose + O2 100% relative activity Phlebiopsis gigantea D-glucosone + H2O2
-
 ?
1.1.3.10 D-glucose + O2 the activation energy for D-glucose activation is 24.7 kJ/mol Phlebiopsis gigantea D-glucosone + H2O2
-
 ?
1.1.3.10 D-glucose + O2 D-glucose is the preferred substrate Phlebiopsis gigantea D-glucosone + H2O2
-
 ?
1.1.3.10 D-xylose + O2 43.7% relative activity to D-glucose Phlebiopsis gigantea D-xylosone + H2O2
-
 ?
1.1.3.10 L-sorbose + O2 96.3% relative activity to D-glucose Phlebiopsis gigantea 5-dehydro-D-fructose + H2O2
-
 ?
1.1.3.10 methyl alpha-D-glucoside + O2 3% relative activity to D-glucose Phlebiopsis gigantea 2-dehydro-alpha-D-methylglucoside + H2O2
-
 ?
1.1.3.10 methyl beta-D-glucoside + O2 26.3% relative activity to D-glucose Phlebiopsis gigantea 2-dehydro-beta-D-methylglucoside + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.10 heterotetramer 2 * 70000 + 2 * 77000, SDS-PAGE, electron microscopy Phlebiopsis gigantea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.10 65
-
-
 Phlebiopsis gigantea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.10 5 8 in 100 mM sodium phosphate Phlebiopsis gigantea
1.1.3.10 5 7.5 in 100 mM potassium phosphate Phlebiopsis gigantea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.10 FAD covalently bound Phlebiopsis gigantea
1.1.3.10 FAD 3.12 mol FAD per mol of enzyme Phlebiopsis gigantea