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Literature summary extracted from

  • Ruelius, H.W.; Kerwin, R.M.; Janssen, F.W.
    Carbohydrate oxidase, a novel enzyme from Polyporus obtusus. I. Isolation and purification (1968), Biochim. Biophys. Acta, 167, 493-500.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.10 Polyporus obtusus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.10 using fractional precipitation with polyethylene glycol and acid treatment Polyporus obtusus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.10 4.4
-
 D-xylose as substrate Polyporus obtusus
1.1.3.10 8.6
-
 D-gluconolactone as substrate Polyporus obtusus
1.1.3.10 9.4
-
 L-sorbose as substrate Polyporus obtusus
1.1.3.10 12.8
-
 D-glucose as substrate Polyporus obtusus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.10 D-fucono-1,5-lactone + O2
-
 Polyporus obtusus 2-keto-D-gluconate + D-araboascorbate + H2O2
-
 ?
1.1.3.10 D-glucose + O2 D-glucose is the preferred substrate Polyporus obtusus D-glucosone + H2O2
-
 ?
1.1.3.10 D-xylose + O2
-
 Polyporus obtusus D-xylosone + H2O2
-
 ?
1.1.3.10 L-sorbose + O2
-
 Polyporus obtusus 5-dehydro-D-fructose + H2O2
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.10 6 8
-
 Polyporus obtusus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.10 4 9
-
 Polyporus obtusus