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Literature summary extracted from

  • Machida, Y.; Nakanishi, T.
    Purification and properties of pyranose oxidase from Coriolus versicolor (1984), Agric. Biol. Chem., 48, 2463-2470.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.10 1,10-phenanthroline
-
 Basidiomycota
1.1.3.10 1,10-phenanthroline 13.3% inhibition Trametes versicolor
1.1.3.10 8-hydroxyquinoline
-
 Basidiomycota
1.1.3.10 8-hydroxyquinoline 13.3% inhibition Trametes versicolor
1.1.3.10 Ag+
-
 Basidiomycota
1.1.3.10 Ag+ 53.3% inhibition Trametes versicolor
1.1.3.10 alpha,alpha'-dipyridyl 6.1% inhibition Trametes versicolor
1.1.3.10 Co2+ 13.3% inhibition Trametes versicolor
1.1.3.10 Cu2+
-
 Basidiomycota
1.1.3.10 Cu2+ 35.7% inhibition Trametes versicolor
1.1.3.10 H2O2 20% inhibition Trametes versicolor
1.1.3.10 Ni2+ 28.6% inhibition Trametes versicolor
1.1.3.10 p-chloromercuribenzoate 50% inhibition Trametes versicolor
1.1.3.10 Zn2+ 13.3% inhibition Trametes versicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.10 0.83
-
 D-glucose
-
 Trametes versicolor
1.1.3.10 1
-
 D-glucose
-
 Lenzites styracinus
1.1.3.10 1.7
-
 D-glucose
-
 Trametes hirsuta
1.1.3.10 1.8
-
 D-glucose
-
 Gloeophyllum sepiarium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.10 68000
-
 4 * 68000, SDS-PAGE Trametes versicolor
1.1.3.10 220000
-
 gel filtration Trametes versicolor

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.10 Basidiomycota
-
 unidentified
-
1.1.3.10 Gloeophyllum sepiarium
-
-
-
1.1.3.10 Lenzites styracinus
-
-
-
1.1.3.10 Trametes hirsuta
-
-
-
1.1.3.10 Trametes versicolor
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.10 using column chromatography on HPA-75, Sepharose 4B, Sephadex G-100 gel filtration and hydroxylapatite chromatography Trametes versicolor

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.10 30.9
-
-
 Trametes versicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.10 D-galactose + O2 0.9% relative activity to D-glucose Trametes versicolor 2-dehydro-D-galactose + H2O2
-
 ?
1.1.3.10 D-glucose + O2
-
 Trametes hirsuta D-glucosone + H2O2
-
 ?
1.1.3.10 D-glucose + O2
-
 Lenzites styracinus D-glucosone + H2O2
-
 ?
1.1.3.10 D-glucose + O2
-
 Gloeophyllum sepiarium D-glucosone + H2O2
-
 ?
1.1.3.10 D-glucose + O2 other electron acceptors: 2,6-dichlorophenolindophenol, cytochrome c Trametes versicolor D-glucosone + H2O2
-
 ?
1.1.3.10 D-glucose + O2 D-glucose is the preferred substrate Trametes versicolor D-glucosone + H2O2
-
 ?
1.1.3.10 D-glucose + O2 D-glucose is the preferred substrate Basidiomycota D-glucosone + H2O2
-
 ?
1.1.3.10 D-mannose + O2 0.9% relative activity to D-glucose Trametes versicolor 2-keto-D-mannose + H2O2
-
 ?
1.1.3.10 D-xylose + O2
-
 Basidiomycota D-xylosone + H2O2
-
 ?
1.1.3.10 D-xylose + O2 2.6% relative activity to D-glucose Trametes versicolor D-xylosone + H2O2
-
 ?
1.1.3.10 L-sorbose + O2
-
 Basidiomycota 5-dehydro-D-fructose + H2O2
-
 ?
1.1.3.10 L-sorbose + O2 3.4% relative activity to D-glucose Trametes versicolor 5-dehydro-D-fructose + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.10 tetramer
-
 Basidiomycota
1.1.3.10 tetramer 4 * 68000, SDS-PAGE Trametes versicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.10 50
-
-
 Trametes versicolor

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.10 60
-
-
 Basidiomycota
1.1.3.10 60
-
 90% of its activity remains after 30 min Trametes versicolor
1.1.3.10 70
-
-
 Basidiomycota
1.1.3.10 70
-
 not stable above Trametes versicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.10 6.2
-
-
 Trametes versicolor

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.10 5 7.4 when kept at 50°C for 30 min Trametes versicolor

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.10 FAD covalently bound Trametes versicolor
1.1.3.10 FAD covalently bound Basidiomycota
1.1.3.10 FAD covalently bound Trametes hirsuta
1.1.3.10 FAD covalently bound Lenzites styracinus
1.1.3.10 FAD covalently bound Gloeophyllum sepiarium