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Literature summary extracted from

  • Izumi, Y.; Furuya, Y.; Yamada, H.
    Purification and properties of pyranose oxidase from Basidiomycetous fungus No. 52 (1990), Agric. Biol. Chem., 54, 1393-1399.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.3.10 NaCl up to 8% Basidiomycota

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.10 1,10-phenanthroline weak inhibitor Basidiomycota
1.1.3.10 8-hydroxyquinoline weak inhibitor Basidiomycota
1.1.3.10 Ag+ strong inhibitor Basidiomycota
1.1.3.10 Cu2+ weak inhibitor Basidiomycota
1.1.3.10 ethanol 17% inhibition by 5% ethanol and 29% inhibition by 10% ethanol Basidiomycota
1.1.3.10 Hg2+ strong inhibitor Basidiomycota
1.1.3.10 NaCl slightly inhibits at concentrations higher than 8% Basidiomycota

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.10 Fe2+ stimulation Basidiomycota

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.10 69000
-
 4 * 69000, SDS-PAGE Basidiomycota
1.1.3.10 300000
-
 gel filtration Basidiomycota

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.10 Basidiomycota
-
 unidentified
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.3.10 glycoprotein 0.7% carbohydrate Basidiomycota

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.10 using column chromatography on HPA-75, Phenyl-Sepharose CL-6B and cellulofine GCL-2000 superfine Basidiomycota

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.10 33.3
-
 purified enzyme Basidiomycota

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.10 6-deoxy-D-glucose + O2
-
 Basidiomycota 2-keto-6-deoxy-D-glucose + H2O2
-
 ?
1.1.3.10 D-fucono-1,5-lactone + O2
-
 Basidiomycota 2-keto-D-gluconate + D-araboascorbate + H2O2
-
 ?
1.1.3.10 D-gluconolactone + O2 activity about 10% that of D-glucose Basidiomycota ? + H2O2
-
 ?
1.1.3.10 D-glucose + O2 D-glucose is the preferred substrate Basidiomycota D-glucosone + H2O2
-
 ?
1.1.3.10 D-xylose + O2 activity about 10% that of D-glucose Basidiomycota D-xylosone + H2O2
-
 ?
1.1.3.10 L-sorbose + O2 activity about 10% that of D-glucose Basidiomycota 5-dehydro-D-fructose + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.10 tetramer 4 * 69000, SDS-PAGE Basidiomycota

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.10 60
-
-
 Basidiomycota

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.10 50
-
 not stable above Basidiomycota
1.1.3.10 60
-
 88% of its activity remains Basidiomycota
1.1.3.10 70
-
 60% of its initial activity remains Basidiomycota

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.10 5.5 8
-
 Basidiomycota

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.10 4
-
 67% of its initial activity remains, citrate buffer Basidiomycota
1.1.3.10 5.5 9 no loss of activity on incubation for 30 min at 50°C Basidiomycota
1.1.3.10 11
-
 78% of its initial activity remains on incubation for 30 min at 50°C, glycine-NaOH buffer Basidiomycota

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.10 FAD covalently bound Basidiomycota