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Literature summary extracted from

  • Nygaard, A.P.
    D(-)-lactic cytochrome c reductase, a flavoprotein from yeast (1961), J. Biol. Chem., 236, 920-925.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.2.4 DL-glycerate strong inhibition Saccharomyces cerevisiae
1.1.2.4 oxalate competitive Saccharomyces cerevisiae
1.1.2.4 oxaloacetate competitive Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.4 0.055
-
 D-lactate
-
 Saccharomyces cerevisiae
1.1.2.4 1.34
-
 D-2-hydroxybutyrate
-
 Saccharomyces cerevisiae
1.1.2.4 2.5
-
 D-2-hydroxyvalerate
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.2.4 mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.2.4 ferricyanide can substitute for cytochrome c Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.4 (R)-lactate + 2 ferricytochrome c Saccharomyces cerevisiae
-
 pyruvate + ferrocytochrome c
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.4 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.4
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.2.4 87
-
 purified enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.4 (R)-lactate + 2 ferricytochrome c
-
 Saccharomyces cerevisiae pyruvate + ferrocytochrome c
-
 ?
1.1.2.4 (R)-lactate + ferricytochrome c
-
 Saccharomyces cerevisiae pyruvate + ferrocytochrome c
-
 ?
1.1.2.4 D-2-hydroxybutyrate + cytochrome c
-
 Saccharomyces cerevisiae 2-oxobutanoate + ferrocytochrome c
-
 ?
1.1.2.4 D-2-hydroxyvalerate + cytochrome c very poor substrate Saccharomyces cerevisiae 2-oxovalerate + ferrocytochrome c
-
 ?
1.1.2.4 DL-glycerate + ferricyanide 10% of the rate of D-lactate oxidation Saccharomyces cerevisiae ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.2.4 250
-
 D-lactate per mol flavin Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.4 6.5
-
 with ferricyanide Saccharomyces cerevisiae
1.1.2.4 7.5
-
 with cytochrome c Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.2.4 5.5 8.5 low activity with cytochrome c at pH 5.5, almost maximum activity at pH 8.5 Saccharomyces cerevisiae
1.1.2.4 5.5 7.5 very low activity with ferricyanide Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.4 2,6-dichlorophenolindophenol can substitute for cytochrome c Saccharomyces cerevisiae
1.1.2.4 cytochrome c
-
 Saccharomyces cerevisiae
1.1.2.4 FAD
-
 Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.2.4 0.0016
-
 oxalate
-
 Saccharomyces cerevisiae
1.1.2.4 0.01
-
 oxaloacetate
-
 Saccharomyces cerevisiae