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Literature summary extracted from

  • Gregolin, C.; Singer, T.P.
    The lactic dehydrogenase of yeast. III. D(-)lactic cytochrome c reductase, a zinc-flavoprotein from aerobic yeast (1963), Biochim. Biophys. Acta, 67, 201-218.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.2.4 very stable in cold Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.2.4 EDTA
-
 Saccharomyces cerevisiae
1.1.2.4 o-phenanthroline very strong Saccharomyces cerevisiae
1.1.2.4 oxalate
-
 Saccharomyces cerevisiae
1.1.2.4 p-Mercuriphenylsulfonate
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.4 0.0054
-
 cytochrome c from horse heart Saccharomyces cerevisiae
1.1.2.4 0.285
-
 D-lactate
-
 Saccharomyces cerevisiae
1.1.2.4 1.4
-
 D-2-hydroxybutyrate
-
 Saccharomyces cerevisiae
1.1.2.4 4.45
-
 oxidized phenazine methosulfate
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.2.4 mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.2.4 Zn2+ very tightly bound to enzyme Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.4 (R)-lactate + 2 ferricytochrome c Saccharomyces cerevisiae
-
 pyruvate + ferrocytochrome c
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.4 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.4
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.2.4 1670
-
 purified enzyme Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
1.1.2.4 -20°C, as ammonium sulfate precipitate Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.4 (R)-lactate + 2 ferricytochrome c
-
 Saccharomyces cerevisiae pyruvate + ferrocytochrome c
-
 ir
1.1.2.4 (R)-lactate + ferricytochrome c specific for D-isomer Saccharomyces cerevisiae pyruvate + ferrocytochrome c
-
 ir
1.1.2.4 D-2-hydroxybutyrate + cytochrome c
-
 Saccharomyces cerevisiae 2-oxobutanoate + ferrocytochrome c
-
 ?
1.1.2.4 D-lactate + oxidized phenazine methosulfate
-
 Saccharomyces cerevisiae pyruvate + reduced phenazine methosulfate + H+
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.2.4 1500
-
 D-lactate per mol flavin Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.4 7
-
 phosphate buffer Saccharomyces cerevisiae
1.1.2.4 7.5
-
 imidazole and Tris buffer Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.2.4 3
-
 stable for short term exposure (30 s) Saccharomyces cerevisiae
1.1.2.4 6.5
-
 very stable Saccharomyces cerevisiae
1.1.2.4 7.5
-
 complete inactivation above pH 7.5 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.4 cytochrome c cytochome c from horse-, beef- and tuna-heart more active than yeast cytochome c Saccharomyces cerevisiae
1.1.2.4 FAD
-
 Saccharomyces cerevisiae
1.1.2.4 phenazine methosulfate can substitute for cytochrome c Saccharomyces cerevisiae