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Literature summary extracted from

  • Tipton, P.A.
    Tartrate dehydrogenase, an enzyme with multiple catalytic activities (2000), Protein Pept. Lett., 7, 323-332.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.93 NH4+ required Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.93 D-malate competitive versus L-tartrate and meso-tartate Pseudomonas putida
1.1.1.93 L-Tartrate competitive versus meso-tartrate and D-malate Pseudomonas putida
1.1.1.93 meso-tartrate competitive versus L-tartrate and D-malate Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.93 0.014
-
 isopropylmalate
-
 Pseudomonas putida
1.1.1.93 0.05
-
 D-malate
-
 Pseudomonas putida
1.1.1.93 0.06
-
 meso-tartrate
-
 Pseudomonas putida
1.1.1.93 1
-
 L-Tartrate
-
 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.93 K+ monovalent cation required, maximal activity with K+ and Rb+ Pseudomonas putida
1.1.1.93 Mg2+ supports catalytic activity Pseudomonas putida
1.1.1.93 Mn2+ required Pseudomonas putida
1.1.1.93 Mn2+ supports catalytic activity Pseudomonas putida
1.1.1.93 Rb+ monovalent cation required, maximal activity with K+ and Rb+ Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.93 L-(+)-tartrate + NAD+ Pseudomonas putida enzyme production is induced by growth on L-(+)-tartrate as the sole carbon source oxaloglycolate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.93 Pseudomonas putida
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.93 tartrate + NAD+ = oxaloglycolate + NADH + H+ A-side dehydrogenase, i.e. the hydride is transferred from the substrate to the pro-R position of C4 of NADH Pseudomonas putida
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.93 D-malate + NAD+
-
 Pseudomonas putida pyruvate + CO2 + NADH D-malate is oxidized to oxaloacetate, which remains bound to the enzyme and undergoes decarboxylation to yield pyruvate ?
1.1.1.93 isopropylmalate + NAD+
-
 Pseudomonas putida ?
-
 ?
1.1.1.93 L-(+)-tartrate + NAD+ enzyme production is induced by growth on L-(+)-tartrate as the sole carbon source Pseudomonas putida oxaloglycolate + NADH
-
 ?
1.1.1.93 L-tartrate + NAD+ oxidation occurs at 2R position Pseudomonas putida oxaloglycolate + NADH + H+ 3R-oxaloglycolate ?
1.1.1.93 meso-tartrate + NAD+ oxidation occurs at 2R oposition Pseudomonas putida oxaloglycolate + NADH + H+ meso-tartrate is oxidized to 3S-oxaloglycolate, followed by decarboxylation to hydroxypyruvate ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.93 0.2
-
 isopropylmalate
-
 Pseudomonas putida
1.1.1.93 0.483
-
 L-Tartrate
-
 Pseudomonas putida
1.1.1.93 0.617
-
 meso-tartrate
-
 Pseudomonas putida
1.1.1.93 19.7
-
 D-malate
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.93 NAD+ cofactor Pseudomonas putida