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Literature summary extracted from

  • Shaw, J.P.; Rekik, M.; Schwager, F.; Harayama, S.
    Kinetic studies on benzyl alcohol dehydrogenase encoded by TOL plasmid pWWO. A member of the zinc-containing long chain alcohol dehydrogenase family (1993), J. Biol. Chem., 268, 10842-10850.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.90 1,10-phenanthroline
-
 Pseudomonas putida
1.1.1.90 additional information product inhibition Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.90 0.04 0.065 benzaldehyde
-
 Pseudomonas putida
1.1.1.90 0.145 0.155 benzyl alcohol
-
 Pseudomonas putida
1.1.1.90 0.195 0.225 NAD+
-
 Pseudomonas putida
1.1.1.90 0.46 1.49 NADH
-
 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.90 KBr activity nearly doubled in presence of salts Pseudomonas putida
1.1.1.90 KCl
-
 Pseudomonas putida
1.1.1.90 KCl activity nearly doubled in presence of salts Pseudomonas putida
1.1.1.90 KNO3 activity nearly doubled in presence of salts Pseudomonas putida
1.1.1.90 NaCl activity nearly doubled in presence of salts Pseudomonas putida
1.1.1.90 NH4Cl activity nearly doubled in presence of salts Pseudomonas putida
1.1.1.90 Zn2+ 1.9 mol of Zn2+ per mol subunit Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.90 38519
-
 2 * 38519, sequence of cDNA Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.90 benzyl alcohol + NAD+ Pseudomonas putida
-
 benzaldehyde + NADH
-
 r
1.1.1.90 benzyl alcohol + NAD+ Pseudomonas putida
-
 benzaldehyde + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.90 Pseudomonas putida
-
-
-
1.1.1.90 Pseudomonas putida P39849
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.90 an aromatic alcohol + NAD+ = an aromatic aldehyde + NADH + H+ ordered bi-bi kinetic mechanism Pseudomonas putida
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.90 3-chlorobenzyl alcohol + NAD+
-
 Pseudomonas putida 3-chlorobenzaldehyde + NADH
-
 r
1.1.1.90 3-methoxybenzyl alcohol + NAD+
-
 Pseudomonas putida 3-methoxybenzaldehyde + NADH
-
 r
1.1.1.90 3-methylbenzyl alcohol + NAD+
-
 Pseudomonas putida 3-methylbenzaldehyde + NADH + H+
-
 ?
1.1.1.90 benzyl alcohol + NAD+
-
 Pseudomonas putida benzaldehyde + NADH
-
 r
1.1.1.90 benzyl alcohol + NAD+
-
 Pseudomonas putida benzaldehyde + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.90 dimer 2 * 38519, sequence of cDNA Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.90 5.7
-
 benzaldehyde + NADH Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.90 NAD+
-
 Pseudomonas putida
1.1.1.90 NADH
-
 Pseudomonas putida