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Literature summary extracted from

  • Takusagawa, Y.; Otagiri, M.; Ui, S.; Ohtsuki, T.; Mimura, A.; Ohkuma, M.; Kudo, T.
    Purification and characterization of L-2,3-butanediol dehydrogenase of Brevibacterium saccharolyticum C-1012 expressed in Escherichia coli (2001), Biosci. Biotechnol. Biochem., 65, 1876-1878.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.76 additional information Ba2+, Ca2+, Mn2+, Mg2+ and Co2+ activate enzyme 1.6-1.8 fold at a concentration of 5 mM Corynebacterium glutamicum

Application

EC Number Application Comment Organism
1.1.1.76 synthesis preparation of chiral acetoinic compounds, enzymic identification for chiral acetoinic compounds or as model enzyme for studying the interrelation between enzymic stereospecificity and structure Corynebacterium glutamicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.76 expression in Escherichia coli JM109/pLBD2-CTC Corynebacterium glutamicum
1.1.1.76 L-butanediol gene containing plasmid pLBD2-CTC is expressed in Escherichia coli JM109 Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.76 2-mercaptoethanol competitive inhibition; competitive inhibitor Corynebacterium glutamicum
1.1.1.76 acetate slight inhibition at 5 mM Corynebacterium glutamicum
1.1.1.76 Cu2+ 5 mM, 89% inhibition; 89% inhibition at 5 mM Corynebacterium glutamicum
1.1.1.76 formate slight inhibition at 5 mM Corynebacterium glutamicum
1.1.1.76 Hg2+ 100% inhibition at 5 mM; 5 mM, 100% inhibition Corynebacterium glutamicum
1.1.1.76 Lactate slight inhibition at 5 mM Corynebacterium glutamicum
1.1.1.76 additional information slight inhibition of 10-20% by organic acids at concentrations of 5 mM: lactate, pyruvate, succinate, acetate and formate Corynebacterium glutamicum
1.1.1.76 pyruvate slight inhibition at 5 mM Corynebacterium glutamicum
1.1.1.76 succinate slight inhibition at 5 mM Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.76 0.07
-
 NAD+ pH 6.0 Corynebacterium glutamicum
1.1.1.76 0.07
-
 NAD+ pH 10.5 Corynebacterium glutamicum
1.1.1.76 0.1
-
 NADH pH 6.0 Corynebacterium glutamicum
1.1.1.76 0.22
-
 (S,S)-butane-2,3-diol pH 6.0 Corynebacterium glutamicum
1.1.1.76 0.22
-
 (S,S)-butane-2,3-diol pH 10.5 Corynebacterium glutamicum
1.1.1.76 0.44
-
 L-acetoin pH 6.0 Corynebacterium glutamicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.76 Ba2+
-
 Corynebacterium glutamicum
1.1.1.76 Ba2+ Ba2+, Ca2+, Mn2+, Mg2+, and Co2+ activate the enzyme 1.6-1.8fold at a concentration of 5 mM Corynebacterium glutamicum
1.1.1.76 Ca2+
-
 Corynebacterium glutamicum
1.1.1.76 Ca2+ Ba2+, Ca2+, Mn2+, Mg2+, and Co2+ activate the enzyme 1.6-1.8fold at a concentration of 5 mM Corynebacterium glutamicum
1.1.1.76 Co2+
-
 Corynebacterium glutamicum
1.1.1.76 Co2+ Ba2+, Ca2+, Mn2+, Mg2+, and Co2+ activate the enzyme 1.6-1.8fold at a concentration of 5 mM Corynebacterium glutamicum
1.1.1.76 Mg2+
-
 Corynebacterium glutamicum
1.1.1.76 Mg2+ Ba2+, Ca2+, Mn2+, Mg2+, and Co2+ activate the enzyme 1.6-1.8fold at a concentration of 5 mM Corynebacterium glutamicum
1.1.1.76 Mn2+
-
 Corynebacterium glutamicum
1.1.1.76 Mn2+ Ba2+, Ca2+, Mn2+, Mg2+, and Co2+ activate the enzyme 1.6-1.8fold at a concentration of 5 mM Corynebacterium glutamicum
1.1.1.76 additional information no significant changes in enzyme activity with 5 mM Ni2+, Zn2+ and Cd2+ Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.76 27107
-
 4 * 30000, SDS-PAGE, 4 * 27107, estimation from gene sequence Corynebacterium glutamicum
1.1.1.76 30000
-
 4 * 30000, SDS-PAGE Corynebacterium glutamicum
1.1.1.76 30000
-
 4 * 30000, SDS-PAGE, 4 * 27107, estimation from gene sequence Corynebacterium glutamicum
1.1.1.76 110000
-
 gel filtration Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.76 Corynebacterium glutamicum
-
 C-1012
-
1.1.1.76 Corynebacterium glutamicum C-1012
-
 C-1012
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.76 recombinant enzyme Corynebacterium glutamicum
1.1.1.76 streptomycin sulfate precipitation, affinity chromatography, gel filtration Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.76 additional information
-
-
 Corynebacterium glutamicum
1.1.1.76 277.6
-
-
 Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.76 2,3-pentanedione + NADH + H+ 7% activity in comparison to L-acetoin Corynebacterium glutamicum ?
-
 r
1.1.1.76 2,3-pentanedione + NADH + H+ 7% activity in comparison to L-acetoin Corynebacterium glutamicum C-1012 ?
-
 r
1.1.1.76 diacetyl + NADH also reduction of 2,3-pentanedione Corynebacterium glutamicum L-acetoin + NAD+
-
 ?
1.1.1.76 diacetyl + NADH also reduction of 2,3-pentanedione Corynebacterium glutamicum C-1012 L-acetoin + NAD+
-
 ?
1.1.1.76 diacetyl + NADH + H+ 35% activity in comparison to L-acetoin Corynebacterium glutamicum ?
-
 r
1.1.1.76 L-acetoin + NADH short chain dehydrogenase reductase family Corynebacterium glutamicum L-2,3-butanediol
-
 r
1.1.1.76 L-acetoin + NADH no oxidadion of several alcohols Corynebacterium glutamicum L-2,3-butanediol
-
 r
1.1.1.76 L-acetoin + NADH short chain dehydrogenase reductase family Corynebacterium glutamicum C-1012 L-2,3-butanediol
-
 r
1.1.1.76 L-acetoin + NADH no oxidadion of several alcohols Corynebacterium glutamicum C-1012 L-2,3-butanediol
-
 r
1.1.1.76 L-acetoin + NADH + H+ 100% activity Corynebacterium glutamicum (S,S)-butane-2,3-diol + NAD+
-
 r
1.1.1.76 additional information not: meso-butanediol, D-butanediol, 2-butanol, 1,2-propanediol, ethanol, acetol, 1,2-butanediol, 1,3-butanediol, n-butanol, n-propanol, D-acetoin, acetol, dihydroxyacetone, 2,4-pentanedione Corynebacterium glutamicum ?
-
 ?
1.1.1.76 additional information not: meso-butanediol, D-butanediol, 2-butanol, 1,2-propanediol, ethanol, acetol, 1,2-butanediol, 1,3-butanediol, n-butanol, n-propanol, D-acetoin, acetol, dihydroxyacetone, 2,4-pentanedione Corynebacterium glutamicum C-1012 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.76 tetramer 4 * 30000, SDS-PAGE Corynebacterium glutamicum
1.1.1.76 tetramer 4 * 30000, SDS-PAGE, 4 * 27107, estimation from gene sequence Corynebacterium glutamicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.76 37
-
 up to, rapidly inactivated over 40°C, 30 min incubation before the enzyme assay Corynebacterium glutamicum
1.1.1.76 37 40 The enzyme is stable up to 37°C but rapidly inactivated over 40°C Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.76 6
-
 reduction of L-acetoin Corynebacterium glutamicum
1.1.1.76 10.5
-
 oxidation of L-butanediol Corynebacterium glutamicum
1.1.1.76 10.5
-
 oxidation of (S,S)-butane-2,3-diol Corynebacterium glutamicum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.76 7 9
-
 Corynebacterium glutamicum
1.1.1.76 7 9 stable Corynebacterium glutamicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.76 NAD+
-
 Corynebacterium glutamicum
1.1.1.76 NADH
-
 Corynebacterium glutamicum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.76 4.28
-
 2-mercaptoethanol Dixon plot Corynebacterium glutamicum
1.1.1.76 4.28
-
 2-mercaptoethanol pH 6.0 Corynebacterium glutamicum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.76 Corynebacterium glutamicum isoelectric focussing
-
 4.9