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Literature summary extracted from
- Separation and quantitation of 2,3-butanediol isomers ((-), (+), and meso) by a combined use of enzyme and gas chromatography (1984), Agric. Biol. Chem., 48, 2837-2838.
No PubMed abstract available
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.1.76 | unstable to repeated freezing and thawing during storage | Corynebacterium glutamicum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.76 | Corynebacterium glutamicum | - |
C-1012 | - |
| 1.1.1.76 | Corynebacterium glutamicum C-1012 | - |
C-1012 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.76 | protamine sufate precipitation | Corynebacterium glutamicum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.76 | 2.8 | - |
- |
Corynebacterium glutamicum |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.76 | -17°C, pH 8.0, stable | Corynebacterium glutamicum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.76 | L-acetoin + NADH | in presence of adequate amounts of NAD+ and hydrazine and in an alkaline condition acetoin formation is much in favour, acetoin concentrations have no appreciable influence on dehydrogenation of L-butanediol | Corynebacterium glutamicum | L-2,3-butanediol | - |
r |  |
| 1.1.1.76 | L-acetoin + NADH | in presence of adequate amounts of NAD+ and hydrazine and in an alkaline condition acetoin formation is much in favour, acetoin concentrations have no appreciable influence on dehydrogenation of L-butanediol | Corynebacterium glutamicum C-1012 | L-2,3-butanediol | - |
r | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.76 | 25 | - |
assay at | Corynebacterium glutamicum |
| 1.1.1.76 | 30 | - |
- |
Corynebacterium glutamicum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.76 | 9.8 | - |
assay at | Corynebacterium glutamicum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.76 | NAD+ | - |
Corynebacterium glutamicum | |
| 1.1.1.76 | NADH | - |
Corynebacterium glutamicum | |
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Release 2023.1 (January 2023)
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