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Literature summary extracted from
- Biosynthesis of pregnane derivatives in somatic embryos of Digitalis lanata (1997), Phytochemistry, 46, 507-513.
No PubMed abstract available
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.1.277 | mitochondrion | - |
Digitalis lanata | 5739 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.50 | 5beta-pregnan-3,20-dione + NAD(P)H | Digitalis lanata | 3alpha-hydroxysteroid-5beta-oxidoreductase activity | 3alpha-hydroxy-5beta-pregnan-20-one + NAD(P)+ | - |
? |  |
| 1.1.1.50 | 5beta-pregnan-3,20-dione + NAD(P)H | Digitalis lanata VIII | 3alpha-hydroxysteroid-5beta-oxidoreductase activity | 3alpha-hydroxy-5beta-pregnan-20-one + NAD(P)+ | - |
? | |
| 1.1.1.277 | 3beta-hydroxy-5beta-pregnane-20-one + NADP+ | Digitalis lanata | biosynthesis and transformation of pregnane derivates | 5beta-pregnane-3,20-dione + NADPH | - |
? | |
| 1.1.1.277 | 3beta-hydroxy-5beta-pregnane-20-one + NADP+ | Digitalis lanata VIII | biosynthesis and transformation of pregnane derivates | 5beta-pregnane-3,20-dione + NADPH | - |
? | |
| 1.1.1.278 | 5alpha-pregnan-3,20-dione + NADPH | Digitalis lanata | - |
3beta-hydroxy-5alpha-pregnane-20-one + NADP+ | - |
r | |
| 1.3.1.22 | progesterone + NADPH + H+ | Digitalis lanata | - |
5alpha-pregnan-3-20-dione + NADP+ | - |
? | |
| 1.3.1.22 | progesterone + NADPH + H+ | Digitalis lanata VIII | - |
5alpha-pregnan-3-20-dione + NADP+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.50 | Digitalis lanata | - |
- |
- |
| 1.1.1.277 | Digitalis lanata | - |
- |
- |
| 1.1.1.278 | Digitalis lanata | - |
- |
- |
| 1.1.1.278 | Digitalis lanata | - |
not light-inducible | - |
| 1.1.1.278 | Digitalis lanata VIII | - |
- |
- |
| 1.3.1.22 | Digitalis lanata | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.50 | embryo | somatic, dark-grown | Digitalis lanata | - |
| 1.1.1.50 | embryo | preembryonic mass | Digitalis lanata | - |
| 1.1.1.50 | leaf | illuminated or light grown leaves | Digitalis lanata | - |
| 1.1.1.277 | embryo | somatic embryo and proembryogenic mass | Digitalis lanata | - |
| 1.1.1.277 | leaf | - |
Digitalis lanata | - |
| 1.1.1.278 | additional information | light and dark-grown | Digitalis lanata | - |
| 1.1.1.278 | additional information | proembryonic mass | Digitalis lanata | - |
| 1.3.1.22 | embryo | somatic | Digitalis lanata | - |
| 1.3.1.22 | leaf | - |
Digitalis lanata | - |
| 1.3.1.22 | additional information | preembryogenic mass | Digitalis lanata | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.50 | 2999 | 4199 | green leaves | Digitalis lanata |
| 1.1.1.277 | additional information | - |
- |
Digitalis lanata |
| 1.3.1.22 | 0.000000012 | - |
activity in dark grown cultures is slightly higher than in light grown cultures | Digitalis lanata |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.50 | 5beta-pregnan-3,20-dione + NAD(P)H | 3alpha-hydroxysteroid-5beta-oxidoreductase activity | Digitalis lanata | 3alpha-hydroxy-5beta-pregnan-20-one + NAD(P)+ | - |
? | |
| 1.1.1.50 | 5beta-pregnan-3,20-dione + NAD(P)H | 3alpha-hydroxysteroid-5beta-oxidoreductase activity | Digitalis lanata VIII | 3alpha-hydroxy-5beta-pregnan-20-one + NAD(P)+ | - |
? | |
| 1.1.1.277 | 3beta-hydroxy-5beta-pregnane-20-one + NADP+ | - |
Digitalis lanata | 5beta-pregnane-3,20-dione + NADPH | - |
r | |
| 1.1.1.277 | 3beta-hydroxy-5beta-pregnane-20-one + NADP+ | biosynthesis and transformation of pregnane derivates | Digitalis lanata | 5beta-pregnane-3,20-dione + NADPH | - |
? | |
| 1.1.1.277 | 3beta-hydroxy-5beta-pregnane-20-one + NADP+ | - |
Digitalis lanata VIII | 5beta-pregnane-3,20-dione + NADPH | - |
r | |
| 1.1.1.277 | 3beta-hydroxy-5beta-pregnane-20-one + NADP+ | biosynthesis and transformation of pregnane derivates | Digitalis lanata VIII | 5beta-pregnane-3,20-dione + NADPH | - |
? | |
| 1.1.1.278 | 5alpha-pregnan-3,20-dione + NAD(P)H | - |
Digitalis lanata | 3beta-hydroxy-5alpha-pregnan-20-one + NAD(P)+ | i.e. 5alpha-pregnan-3beta-ol-20-one | r | |
| 1.1.1.278 | 5alpha-pregnan-3,20-dione + NADPH | - |
Digitalis lanata | 3beta-hydroxy-5alpha-pregnane-20-one + NADP+ | - |
r | |
| 1.3.1.22 | progesterone + NADPH + H+ | - |
Digitalis lanata | 5alpha-pregnan-3-20-dione + NADP+ | - |
? | |
| 1.3.1.22 | progesterone + NADPH + H+ | - |
Digitalis lanata VIII | 5alpha-pregnan-3-20-dione + NADP+ | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.278 | 3beta-hydroxysteroid-5alpha-oxidoreductase | - |
Digitalis lanata |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.278 | 37 | - |
assay at | Digitalis lanata |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.278 | 7.5 | - |
assay at | Digitalis lanata |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.277 | NADPH | - |
Digitalis lanata | |
| 1.1.1.278 | NAD+ | - |
Digitalis lanata | |
| 1.1.1.278 | NADH | - |
Digitalis lanata | |
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Release 2023.1 (January 2023)
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