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Literature summary extracted from

  • Seitz, H.U.; Gaertner, D.E.
    Enzymes in cardenolide-accumulating shoot cultures of Digitalis purpurea (1994), Plant Cell Tissue Organ Cult., 38, 337-344.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.50 0.235
-
 5beta-Pregnan-3alpha-ol-20-one cytosolic NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase Digitalis purpurea
1.1.1.50 0.4
-
 NADH cytosolic NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase Digitalis purpurea
1.1.1.50 0.91
-
 NADPH cytosolic NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase Digitalis purpurea
1.1.1.50 1.33
-
 NADPH microsomal NADPH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase Digitalis purpurea
1.1.1.277 0.00139
-
 NADH
-
 Digitalis purpurea
1.1.1.277 0.0171
-
 5beta-pregnane-3,20-dione
-
 Digitalis purpurea
1.1.1.277 0.3
-
 NADPH
-
 Digitalis purpurea
1.3.1.22 0.03
-
 progesterone
-
 Digitalis purpurea
1.3.1.22 0.13
-
 NADPH
-
 Digitalis purpurea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.50 cytosol NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase activity, 97% in the cytosol Digitalis purpurea 5829
-
1.1.1.50 microsome low activity of NADPH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase activity Digitalis purpurea
-
-
1.1.1.277 soluble
-
 Digitalis purpurea
-
-
1.3.1.22 endoplasmic reticulum
-
 Digitalis purpurea 5783
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.50 5alpha-pregnan-3,20-dione + NAD(P)H + H+ Digitalis purpurea 5alpha-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase activity, NADPH and NADH possible as cofactors 5alpha-pregnane-3alpha-ol-20-one + NAD(P)+
-
 ?
1.1.1.50 5beta-pregnan-3,20-dione + NAD(P)H Digitalis purpurea 3alpha-hydroxysteroid-5beta-oxidoreductase activity 3alpha-hydroxy-5beta-pregnan-20-one + NAD(P)+
-
 r
1.1.1.277 3beta-hydroxy-5beta-pregnane-20-one + NADP+ Digitalis purpurea biosynthesis of cardenolides 5beta-pregnane-3,20-dione + NADPH
-
 ?
1.3.1.22 progesterone + NADPH + H+ Digitalis purpurea
-
 5alpha-pregnan-3-20-dione + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.50 Digitalis purpurea
-
-
-
1.1.1.277 Digitalis purpurea
-
-
-
1.3.1.22 Digitalis purpurea
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.50 shoot culture Digitalis purpurea
-
1.1.1.277 additional information no activity in undifferentiated cell cultures Digitalis purpurea
-
1.1.1.277 shoot
-
 Digitalis purpurea
-
1.3.1.22 shoot
-
 Digitalis purpurea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.50 3alpha-hydroxy-5beta-pregnan-20-one + NADP+
-
 Digitalis purpurea 5beta-pregnan-3,20-dione + NADPH
-
 r
1.1.1.50 5alpha-pregnan-3,20-dione + NAD(P)H + H+ 5alpha-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase activity, NADPH and NADH possible as cofactors Digitalis purpurea 5alpha-pregnane-3alpha-ol-20-one + NAD(P)+
-
 ?
1.1.1.50 5beta-pregnan-3,20-dione + NAD(P)H 3alpha-hydroxysteroid-5beta-oxidoreductase activity Digitalis purpurea 3alpha-hydroxy-5beta-pregnan-20-one + NAD(P)+
-
 r
1.1.1.50 5beta-pregnane-3,20-dione + NADH + H+
-
 Digitalis purpurea 5beta-pregnan-3alpha-ol-20-one + NAD+
-
 r
1.1.1.277 3beta-hydroxy-5beta-pregnane-20-one + NADP+ stereospecific Digitalis purpurea 5beta-pregnane-3,20-dione + NADPH
-
 r
1.1.1.277 3beta-hydroxy-5beta-pregnane-20-one + NADP+ biosynthesis of cardenolides Digitalis purpurea 5beta-pregnane-3,20-dione + NADPH
-
 ?
1.1.1.277 5beta-pregnan-3beta-ol-20-one + NADH + H+
-
 Digitalis purpurea ?
-
 r
1.3.1.22 progesterone + NADPH + H+
-
 Digitalis purpurea 5alpha-pregnan-3-20-dione + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.50 3alpha-hydroxysteroid-5beta-oxidoreductase
-
 Digitalis purpurea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.50 NAD+
-
 Digitalis purpurea
1.1.1.50 NADH cytosolic 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase activity Digitalis purpurea
1.1.1.50 NADPH NADPH-linked microsomal 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase shows very low activity only with NADPH Digitalis purpurea
1.1.1.277 NADH affinity to NADH is lower than to NADPH Digitalis purpurea
1.1.1.277 NADPH
-
 Digitalis purpurea