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Literature summary extracted from

  • Lin, H.K.; Jez, J.M.; Schlegel, B.P.; Peehl, D.M.; Pachter, J.A.; Penning, T.M.
    Expression and characterization of recombinant type 2: 3alpha-hydroxysteroid dehydrogenase (HSD) from human prostate: demonstration of bifunctional 3alpha/17beta-HSD activity and cellular distribution (1997), Mol. Endocrinol., 11, 1971-1984.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.50 expression of type II isoform in Escherichia coli Homo sapiens
1.1.1.209 expressed in Escherichia coli Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.50 0.004
-
 androstanedione NADPH, detection spectrophotometrically Homo sapiens
1.1.1.50 0.006
-
 5alpha-dihydrotestosterone NADP+, detection radiometrically Homo sapiens
1.1.1.50 0.012
-
 testosterone NADP+, detection radiometrically Homo sapiens
1.1.1.50 0.017
-
 (2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide NADP+, detection spectrophotometrically Homo sapiens
1.1.1.50 0.018
-
 5alpha-dihydrotestosterone NADPH, detection radiometrically Homo sapiens
1.1.1.50 0.019
-
 5alpha-dihydrotestosterone NADPH, detection spectrophotometrically Homo sapiens
1.1.1.50 0.019
-
 (2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide NADP+, detection radio- or fluorometrically Homo sapiens
1.1.1.50 0.021
-
 9,10-phenanthrenequinone NADPH, detection spectrophotometrically Homo sapiens
1.1.1.50 0.048
-
 androsterone NADP+, detection radiometrically Homo sapiens
1.1.1.50 0.087
-
 4-nitrobenzaldehyde NADPH, detection spectrophotometrically Homo sapiens
1.1.1.209 0.012
-
 testosterone
-
 Homo sapiens
1.1.1.209 0.019
-
 (2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide
-
 Homo sapiens
1.1.1.209 0.026
-
 5alpha-dihydrotestosterone
-
 Homo sapiens
1.1.1.209 0.048
-
 androsterone
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.50 additional information Homo sapiens type 3 isoform in liver virtually identical with bile acid-binding protein ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.50 Homo sapiens
-
 type 2 isoform
-
1.1.1.209 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.50 recombinant from Escherichia coli Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.50 a 3alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H + H+ enzyme isoforms bind substrate in different ways Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.50 colon
-
 Homo sapiens
-
1.1.1.50 heart
-
 Homo sapiens
-
1.1.1.50 kidney
-
 Homo sapiens
-
1.1.1.50 liver
-
 Homo sapiens
-
1.1.1.50 lung
-
 Homo sapiens
-
1.1.1.50 additional information type 2 isoform is expressed in several tissues Homo sapiens
-
1.1.1.50 ovary
-
 Homo sapiens
-
1.1.1.50 prostate gland
-
 Homo sapiens
-
1.1.1.50 skeletal muscle
-
 Homo sapiens
-
1.1.1.50 small intestine
-
 Homo sapiens
-
1.1.1.50 thymus
-
 Homo sapiens
-
1.1.1.209 brain
-
 Homo sapiens
-
1.1.1.209 liver
-
 Homo sapiens
-
1.1.1.209 placenta
-
 Homo sapiens
-
1.1.1.209 prostate gland
-
 Homo sapiens
-
1.1.1.209 spleen
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.50 0.0005
-
 substrate androsterone with cofactor NADP+, detection radiometrically Homo sapiens
1.1.1.50 0.0013
-
 substrate 5alpha-dihydrotestosterone with cofactor NADP+, detection radiometrically Homo sapiens
1.1.1.50 0.0025
-
 substrate 5alpha-dihydrotestosterone with cofactor NADPH, detection spectrophotometrically Homo sapiens
1.1.1.50 0.0042
-
 substrate 5alpha-dihydrotestosterone with cofactor NADPH, detection radiometrically Homo sapiens
1.1.1.50 0.0048
-
 substrate 3alpha-androstanediol with cofactor NADP+, detection spectrophotometrically Homo sapiens
1.1.1.50 0.0085
-
 substrate 3alpha-androstanediol with cofactor NADP+, detection radiometrically Homo sapiens
1.1.1.50 0.0111
-
 substrate androstanedione with cofactor NADPH, detection spectrophotometrically Homo sapiens
1.1.1.50 0.0134
-
 substrate 3alpha-androstanediol with cofactor NADP+, detection fluorometrically Homo sapiens
1.1.1.50 0.13
-
 substrate 4-nitrobenzaldehyde with cofactor NADPH, detection spectrophotometrically Homo sapiens
1.1.1.50 0.538
-
 substrate 9,10-phenanthrenequinone with cofactor NADPH, detection spectrophotometrically Homo sapiens
1.1.1.209 0.0035
-
 reduction of 5alpha-dihydrotestosteerone, cofactor NADPH, pH 6.0 Homo sapiens
1.1.1.209 0.0063
-
 oxidation of 3alpha-androstanediol, cofactor NADP+, pH 9.0 Homo sapiens
1.1.1.209 0.0253
-
 reduction of 5alpha-androstanedione, cofactor NADPH, pH 6.0 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.50 (2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide + NADP+
-
 Homo sapiens ?
-
 ?
1.1.1.50 5alpha-androstan-3alpha,17beta-diol + NAD(P)+
-
 Homo sapiens 17beta-hydroxy-5alpha-androstan-3-one + NADPH
-
 r
1.1.1.50 5alpha-androstan-3alpha,17beta-diol + NAD(P)H
-
 Homo sapiens 5alpha-dihydrotestosterone + NAD(P)+
-
 r
1.1.1.50 5alpha-dihydrotestosterone + NAD(P)H + H+
-
 Homo sapiens 5alpha-androstan-3alpha,17beta-diol + NAD(P)+
-
 r
1.1.1.50 5alpha-dihydrotestosterone + NAD(P)H + H+
-
 Homo sapiens (3beta,5alpha,17beta)-androstane-3,17-diol + NAD(P)+
-
 r
1.1.1.50 5beta-androstan-3,17-dione + NAD(P)H
-
 Homo sapiens 5beta-androstan-17one-3-ol + NAD(P)+
-
 r
1.1.1.50 5beta-androstan-3alpha,17beta-diol + NADP+
-
 Homo sapiens 17beta-hydroxy-5beta-androstan-3-one + NADPH
-
 r
1.1.1.50 9,10-phenanthrenequinone + NADPH
-
 Homo sapiens ?
-
 ?
1.1.1.50 androsterone + NAD(P)+
-
 Homo sapiens 5alpha-androstan-3,17-dione + NAD(P)H
-
 r
1.1.1.50 androsterone + NADP+
-
 Homo sapiens androstane-3,17-dione + NADPH
-
 ?
1.1.1.50 additional information can not catalyze conversion of 3alpha-androstanediol to 5alpha-dihydrotestosterone Homo sapiens ?
-
 ?
1.1.1.50 additional information type 3 isoform in liver virtually identical with bile acid-binding protein Homo sapiens ?
-
 ?
1.1.1.50 p-nitrobenzaldehyde + NAD(P)H
-
 Homo sapiens p-nitrobenzylalcohol + NAD(P)+
-
 ?
1.1.1.50 testosterone + NAD(P)+
-
 Homo sapiens androstenedione + NAD(P)H + H+
-
 ?
1.1.1.209 (2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide + NADPH + H+ i.e., 5alpha-dihydrotestosterone Homo sapiens 3alpha-androstanediol + NADP+
-
 ?
1.1.1.209 3alpha-androstanediol + NAD(P)+
-
 Homo sapiens androsterone + NAD(P)H
-
 r
1.1.1.209 5alpha-dihydrotestosterone + NAD(P)H
-
 Homo sapiens androstanedione + NAD(P)+
-
 r
1.1.1.209 testosterone + NAD(P)+
-
 Homo sapiens androstenedione + NAD(P)H + H+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.50 0.00783
-
 androsterone
-
 Homo sapiens
1.1.1.50 0.0398
-
 5alpha-dihydrotestosterone detection fluorometrically Homo sapiens
1.1.1.50 0.0757
-
 testosterone
-
 Homo sapiens
1.1.1.50 0.0965
-
 5alpha-dihydrotestosterone detection spectrophotometrically Homo sapiens
1.1.1.50 0.114
-
 5alpha-dihydrotestosterone detection radiometrically Homo sapiens
1.1.1.50 0.203
-
 (2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide detection spectrophotometrically Homo sapiens
1.1.1.50 0.32
-
 (2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide detection radiometrically Homo sapiens
1.1.1.50 0.507
-
 (2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide detection fluorometrically Homo sapiens
1.1.1.50 1.08
-
 4-nitrobenzaldehyde
-
 Homo sapiens
1.1.1.50 1.9
-
 androstanedione detection spectrophotometrically Homo sapiens
1.1.1.50 18.9
-
 9,10-phenanthrenequinone
-
 Homo sapiens
1.1.1.209 0.000367
-
 androsterone
-
 Homo sapiens
1.1.1.209 0.0009
-
 testosterone
-
 Homo sapiens
1.1.1.209 0.003
-
 5alpha-dihydrotestosterone
-
 Homo sapiens
1.1.1.209 0.00617
-
 (2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.50 NADP+
-
 Homo sapiens
1.1.1.50 NADPH
-
 Homo sapiens