Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Biswas, M.G.; Russel, D.W.
    Expression cloning and characterization of oxidative 17beta- and 3alpha-hydroxysteroid dehydrogenase from rat and human prostate (1997), J. Biol. Chem., 272, 15959-15966.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.50 expression in Escherichia coli Homo sapiens
1.1.1.50 expression in Escherichia coli Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.50 0.0001
-
 androsterone
-
 Homo sapiens
1.1.1.50 0.0001
-
 androsterone
-
 Rattus norvegicus
1.1.1.50 0.0001
-
 5alpha-dihydrotestosterone
-
 Homo sapiens
1.1.1.50 0.0001
-
 3alpha-adiol
-
 Rattus norvegicus
1.1.1.50 0.0028
-
 testosterone
-
 Rattus norvegicus
1.1.1.50 0.0033
-
 testosterone
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.50 5alpha-dihydrotestosterone + NAD(P)H + H+ Homo sapiens discussion of physiological role 5alpha-androstane-3alpha,17beta-diol + NAD(P)+
-
 r
1.1.1.50 5alpha-dihydrotestosterone + NAD(P)H + H+ Rattus norvegicus discussion of physiological role 5alpha-androstane-3alpha,17beta-diol + NAD(P)+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.50 Homo sapiens
-
-
-
1.1.1.50 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.50 liver
-
 Homo sapiens
-
1.1.1.50 liver
-
 Rattus norvegicus
-
1.1.1.50 prostate gland
-
 Homo sapiens
-
1.1.1.50 prostate gland
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.50 0.005
-
-
 Rattus norvegicus
1.1.1.50 0.011
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.50 3alpha-adiol + NAD(P)+
-
 Homo sapiens dihydrotestosterone + NAD(P)H
-
 r
1.1.1.50 3alpha-adiol + NAD(P)+
-
 Rattus norvegicus dihydrotestosterone + NAD(P)H
-
 r
1.1.1.50 5alpha-dihydrotestosterone + NAD(P)H + H+
-
 Homo sapiens 5alpha-androstan-3alpha,17beta-diol + NAD(P)+
-
 r
1.1.1.50 5alpha-dihydrotestosterone + NAD(P)H + H+
-
 Rattus norvegicus 5alpha-androstan-3alpha,17beta-diol + NAD(P)+
-
 r
1.1.1.50 5alpha-dihydrotestosterone + NAD(P)H + H+
-
 Homo sapiens (3beta,5alpha,17beta)-androstane-3,17-diol + NAD(P)+
-
 r
1.1.1.50 5alpha-dihydrotestosterone + NAD(P)H + H+
-
 Rattus norvegicus (3beta,5alpha,17beta)-androstane-3,17-diol + NAD(P)+
-
 r
1.1.1.50 5alpha-dihydrotestosterone + NAD(P)H + H+ discussion of physiological role Homo sapiens 5alpha-androstane-3alpha,17beta-diol + NAD(P)+
-
 r
1.1.1.50 5alpha-dihydrotestosterone + NAD(P)H + H+ discussion of physiological role Rattus norvegicus 5alpha-androstane-3alpha,17beta-diol + NAD(P)+
-
 r
1.1.1.50 5beta-dihydrotestosterone + NAD(P)H
-
 Homo sapiens 5beta-androstan-3alpha,17beta-diol + NAD(P)+
-
 ?
1.1.1.50 5beta-dihydrotestosterone + NAD(P)H
-
 Rattus norvegicus 5beta-androstan-3alpha,17beta-diol + NAD(P)+
-
 ?
1.1.1.50 androsterone + NAD(P)+
-
 Homo sapiens 5alpha-androstan-3,17-dione + NAD(P)H
-
 r
1.1.1.50 androsterone + NAD(P)+
-
 Rattus norvegicus 5alpha-androstan-3,17-dione + NAD(P)H
-
 r
1.1.1.50 estradiol + NAD(P)H
-
 Homo sapiens estrone + NAD(P)+
-
 ?
1.1.1.50 estradiol + NAD(P)H
-
 Rattus norvegicus estrone + NAD(P)+
-
 ?
1.1.1.50 testosterone + NAD(P)+
-
 Homo sapiens androstenedione + NAD(P)H + H+
-
 ?
1.1.1.50 testosterone + NAD(P)+
-
 Rattus norvegicus androstenedione + NAD(P)H + H+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.50 0.0167
-
 stanolone
-
 Homo sapiens
1.1.1.50 0.0333
-
 androsterone
-
 Rattus norvegicus
1.1.1.50 0.0833
-
 3alpha-adiol
-
 Rattus norvegicus
1.1.1.50 0.133
-
 androsterone
-
 Homo sapiens
1.1.1.50 0.183
-
 3alpha-adiol
-
 Homo sapiens