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Literature summary extracted from

  • Hara, A.; Inoue, Y.; Nakagawa, M.; Naganeo, F.; Sawada, H.
    Purification and characterization of NADP+-dependent 3alpha-hydroxysteroid dehydrogenase from mouse liver cytosol (1988), J. Biochem., 103, 1027-1034.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.50 glycerol: 30%, partially stabilizes Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.50 11beta,17alpha,21-trihydroxy-4-pregnen-3,20-dione
-
 Mus musculus
1.1.1.50 11beta,21-dihydroxy-4-pregnen-3,20-dione
-
 Mus musculus
1.1.1.50 17alpha,21-Dihydroxy-1,4-pregnadien-3,11,20-trione
-
 Mus musculus
1.1.1.50 17alpha,21-dihydroxy-4-pregnen-3,11,20-trione
-
 Mus musculus
1.1.1.50 17alpha-Hydroxy-4-androsten-3-one
-
 Mus musculus
1.1.1.50 17alpha-hydroxy-4-pregnen-3,20-dione
-
 Mus musculus
1.1.1.50 17beta-hydroxy-4-androsten-3-one
-
 Mus musculus
1.1.1.50 2alpha-Bromo-17beta-hydroxy-5alpha-androstan-3-one
-
 Mus musculus
1.1.1.50 4-androsten-3,17-dione
-
 Mus musculus
1.1.1.50 4-pregnen-3,20-dione
-
 Mus musculus
1.1.1.50 6alpha-Methylprednisolone
-
 Mus musculus
1.1.1.50 Bethamethasone
-
 Mus musculus
1.1.1.50 deoxycorticosterone
-
 Mus musculus
1.1.1.50 dexamethasone
-
 Mus musculus
1.1.1.50 Dienstrol
-
 Mus musculus
1.1.1.50 Hexestrol
-
 Mus musculus
1.1.1.50 Hg2+
-
 Mus musculus
1.1.1.50 indomethacin
-
 Mus musculus
1.1.1.50 Medroxyprogesterone acetate
-
 Mus musculus
1.1.1.50 additional information product inhibition Mus musculus
1.1.1.50 Prednisolone
-
 Mus musculus
1.1.1.50 prostaglandin A1
-
 Mus musculus
1.1.1.50 Prostaglandin D2
-
 Mus musculus
1.1.1.50 prostaglandin E2
-
 Mus musculus
1.1.1.50 prostaglandin F2alpha
-
 Mus musculus
1.1.1.50 Stilbestrol
-
 Mus musculus
1.1.1.50 Triamcinolone
-
 Mus musculus
1.1.1.50 Zomepirac
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.50 0.00035 0.00049 5beta-androstan-3,17-dione also in this range: 5beta-pregnan-3,20-dione, 5alpha-androstan-3,17-dione Mus musculus
1.1.1.50 0.0007 0.00075 3-oxo-5beta-cholan-24-oic acid
-
 Mus musculus
1.1.1.50 0.00097
-
 NADPH
-
 Mus musculus
1.1.1.50 0.0012 0.0017 17beta-hydroxy-5beta-androstan-3-one also in this range: 17alpha,21-dihydroxy-5beta-pregnan-3,11,20-trione, acenaphthequinone Mus musculus
1.1.1.50 0.002 0.0024 17beta-hydroxy-5alpha-androstan-3-one
-
 Mus musculus
1.1.1.50 0.0052 0.0059 glycolithocholic acid
-
 Mus musculus
1.1.1.50 0.007 0.0083 5alpha-dihydrotestosterone also in this range: 3alpha-hydroxy-5beta-pregnan-17-one Mus musculus
1.1.1.50 0.0095
-
 androsterone
-
 Mus musculus
1.1.1.50 0.01 0.025 cholic acid 3alpha-hydroxy-5beta-androstan-17-one, 3alpha,21-dihydroxy-5beta-pregnan-20-one, 5beta-androstan-3alpha,17beta-diol, 5alpha-androstan-3alpha,17beta-diol, NADP+ Mus musculus
1.1.1.50 0.03 0.077 Camphorquinone 4-nitrobenzaldehyde, 2,3-heptanedione Mus musculus
1.1.1.50 0.2 0.8 4-Nitroacetophenone also in this range: 2,3-hexanedione, 1-phenyl-1,2-propanedione, 2,3-pentanedione, pyridine-4-aldehyde, 2,3-butanedione Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.50 cytosol
-
 Mus musculus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.50 32500 34000 gel filtration Mus musculus
1.1.1.50 34000
-
 1 * 34000, SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.50 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.50
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.50 liver
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.50 6.1
-
-
 Mus musculus

Storage Stability

EC Number Storage Stability Organism
1.1.1.50 -20°C, purified enzyme, at least 2 weeks, crude extract loses 80% activity at 4°C in 3 days Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.50 3alpha,17alpha,21-trihydroxy-5beta-pregnan-11,20-dione + NADP+
-
 Mus musculus 17alpha,21-dihydroxy-5beta-pregnan-3,11,20-trione + NADPH
-
 r
1.1.1.50 3alpha,21-dihydroxy-5beta-pregnan-20-one + NADP+
-
 Mus musculus 21-hydroxy-5beta-pregnan-3,20-dione + NADPH
-
 r
1.1.1.50 3alpha-hydroxy-5beta-androstan-17-one + NADP+
-
 Mus musculus 5beta-androstan-3,17-dione + NADPH
-
 r
1.1.1.50 3alpha-hydroxy-5beta-pregnan-20-one + NADP+
-
 Mus musculus 5beta-pregnan-3,20-dione + NADPH
-
 r
1.1.1.50 4-nitroacetophenone + NADPH + H+
-
 Mus musculus 1-(4-nitrophenyl)ethanol + NADP+
-
 ?
1.1.1.50 5alpha-androstan-3alpha,17beta-diol + NAD(P)+
-
 Mus musculus 17beta-hydroxy-5alpha-androstan-3-one + NADPH
-
 r
1.1.1.50 5beta-androstan-3alpha,17beta-diol + NADP+
-
 Mus musculus 17beta-hydroxy-5beta-androstan-3-one + NADPH
-
 r
1.1.1.50 androsterone + NAD(P)+ i.e. 3alpha-hydroxy-5alpha-androstan-17-one Mus musculus 5alpha-androstan-3,17-dione + NAD(P)H
-
 r
1.1.1.50 camphorquinone + NADPH
-
 Mus musculus ?
-
 ?
1.1.1.50 cholic acid + NADPH + H+
-
 Mus musculus ?
-
 r
1.1.1.50 glycolithocholic acid + NADP+ i.e. 3alpha-hydroxy-5beta-cholanoyl glycine Mus musculus 3-oxo-glycocholanoic acid + NADPH
-
 ?
1.1.1.50 additional information reduction of carbonyl compounds e.g. quinones, aliphatic-chain diketones, ketones Mus musculus ?
-
 ?
1.1.1.50 additional information absolutely specific for 3alpha-hydroxy group of C19, C21 and C24 steroids Mus musculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.50 monomer 1 * 34000, SDS-PAGE Mus musculus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.50 16 36 increase of activity over range Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.50 6.5
-
 reduction with NADPH Mus musculus
1.1.1.50 7
-
 reduction of 3-oxo-steroid Mus musculus
1.1.1.50 9.5
-
 oxidation of 3alpha-hydroxysteroids Mus musculus