EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.229 | 1,10-phenanthroline | 30% of activity at 10 mM | Torulaspora delbrueckii | |
1.1.1.229 | cysteine | complete inhibition at 10 mM | Torulaspora delbrueckii | |
1.1.1.229 | FMN | slight inhibition at 0.2 mM | Torulaspora delbrueckii | |
1.1.1.229 | high salt concentration | 1M NaCl or KCl | Torulaspora delbrueckii | |
1.1.1.229 | KCN | complete inhibition at 10 mM | Torulaspora delbrueckii | |
1.1.1.229 | monoiodoacetate | complete inhibition at 10 mM | Torulaspora delbrueckii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.229 | 1.25 | - |
Diethyl 2-methyl-3-oxosuccinate | - |
Torulaspora delbrueckii | |
1.1.1.229 | 111 | - |
Diethyl 2-methyl-3-hydroxysuccinate | - |
Torulaspora delbrueckii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.229 | Ag+ | strong inhibitor | Torulaspora delbrueckii | |
1.1.1.229 | Cu2+ | strong inhibitor | Torulaspora delbrueckii | |
1.1.1.229 | Fe3+ | strong inhibitor | Torulaspora delbrueckii | |
1.1.1.229 | K+ | strongly inhibitory at 1 M | Torulaspora delbrueckii | |
1.1.1.229 | Na+ | strongly inhibitory at 1 M | Torulaspora delbrueckii | |
1.1.1.229 | Zn2+ | slight inhibition | Torulaspora delbrueckii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.229 | 61000 | - |
gel filtration | Torulaspora delbrueckii |
1.1.1.229 | 63000 | - |
SDS-PAGE | Torulaspora delbrueckii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.229 | diethyl 2-methyl-3-oxosuccinate + NADPH | Torulaspora delbrueckii | asymmetric reduction in yeast, the reduction is strongly preferred over the weak oxidation activity | diethyl 2-methyl-3-hydroxysuccinate + NADP+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.229 | Torulaspora delbrueckii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.229 | - |
Torulaspora delbrueckii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.229 | 0.004 | - |
crude extract | Torulaspora delbrueckii |
1.1.1.229 | 0.649 | - |
purified enzyme | Torulaspora delbrueckii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.229 | diethyl 2-methyl-3-oxosuccinate + NADPH | the reduction is strongly preferred, the catalyzed reaction is an asymmetric reduction, best substrate | Torulaspora delbrueckii | diethyl 2-methyl-3-hydroxysuccinate + NADP+ | alpha-methyl-beta-hydroxy ester, the enzyme catalyzes an asymmetric reduction, the product is a mixture of the (2R,3R)-syn- and (2S,3R)-anti-beta-hydroxyester | r | |
1.1.1.229 | diethyl 2-methyl-3-oxosuccinate + NADPH | asymmetric reduction in yeast, the reduction is strongly preferred over the weak oxidation activity | Torulaspora delbrueckii | diethyl 2-methyl-3-hydroxysuccinate + NADP+ | - |
r | |
1.1.1.229 | dimethyl 2-methyl-3-oxosuccinate + NADPH | poor substrate | Torulaspora delbrueckii | dimethyl 2-methyl-3-hydroxysuccinate + NADP+ | - |
? | |
1.1.1.229 | additional information | no aldehydes or several synthetic substrates are reduced, such as: acetaldehyde, formaldehyde, isobutanal, n-pentanal, n-hexanal, n-heptanal, 4-hydroxybenzaldehyde, 4-dimethylaminobenzaldehyde, benzaldehyde, furfural, 2-methyl-3-oxobutanoic acid benzyl ester, 2-chloro-3-oxobutanoic acid ethyl ester, 3-phenyl-2-oxobutanoic acid methyl ester, 2-methyl-3-oxobutanoic acid ethyl ester, 3-(2'-furyl)-2-methyl-3-oxopropionic acid methyl ester, none of the following alcohols or other substrates is oxidized: methanol, ethanol, n-propanol, isopropanol, n-butanol, isobutanol, n-pentanol, isopentanol, benzyl alcohol, glycerin, shikimic acid, 2-deoxy-D-glucose, glucose 6-phosphate, in the presence of NAD+ or NADP+ | Torulaspora delbrueckii | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.229 | monomer | - |
Torulaspora delbrueckii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.229 | 50 | - |
- |
Torulaspora delbrueckii |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.229 | 30 | 60 | more than 70% of maximal activity at both temperatures | Torulaspora delbrueckii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.229 | 40 | - |
very unstable at temperatures above 40°C for 10 min | Torulaspora delbrueckii |
1.1.1.229 | 60 | - |
complete loss of activity | Torulaspora delbrueckii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.229 | 6 | - |
sharp peak | Torulaspora delbrueckii |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.229 | 4 | 8 | less than 10% of activity at both pH | Torulaspora delbrueckii |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.229 | 6 | 9 | stable for 30 min | Torulaspora delbrueckii |