BRENDA - Enzyme Database show

Enzymatic formation of prostaglandin F2 alpha from prostaglandin H2 and D2. Purification and properties of prostaglandin F synthetase from bovine lung

Watanabe, K.; Yoshida, R.; Shimizu, T.; Hayaishi, O.; J. Biol. Chem. 260, 7035-7041 (1985)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.188
0.0007
-
9,10-phenanthrenequinone
native lung prostaglandin F synthase
Bos taurus
1.1.1.188
0.01
-
prostaglandin H2
native lung prostaglandin F synthase
Bos taurus
1.1.1.188
0.013
-
4-Nitroacetophenone
lung enzyme
Bos taurus
1.1.1.188
0.018
-
Hydrindantin
lung enzyme
Bos taurus
1.1.1.188
0.08
-
Phenylglyoxal
lung enzyme
Bos taurus
1.1.1.188
0.1
-
Prostaglandin D2
lung enzyme
Bos taurus
1.1.1.188
0.125
-
4-nitrobenzaldehyde
lung enzyme
Bos taurus
1.1.1.188
0.25
-
Prostaglandin A2
lung enzyme
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.188
30500
-
SDS-PAGE, sedimentation equilibrium and gel filtration
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.188
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.188
-
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.188
lung
two different active sites
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.188
0.15
-
prostaglandin F2alpha as product
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH
-
389460
Bos taurus
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
-
1.1.1.188
9,10-phenanthrenequinone + NADPH
-
389460
Bos taurus
?
-
-
-
?
1.1.1.188
hydrindantin + NADPH
-
389460
Bos taurus
?
-
-
-
-
1.1.1.188
phenylglyoxal + NADPH
-
389460
Bos taurus
?
-
-
-
-
1.1.1.188
prostaglandin A2 + NADPH
-
389460
Bos taurus
?
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
-
389460
Bos taurus
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.188
37
-
assay at
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.188
6
7
-
Bos taurus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADPH
-
Bos taurus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADPH
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.188
0.0007
-
9,10-phenanthrenequinone
native lung prostaglandin F synthase
Bos taurus
1.1.1.188
0.01
-
prostaglandin H2
native lung prostaglandin F synthase
Bos taurus
1.1.1.188
0.013
-
4-Nitroacetophenone
lung enzyme
Bos taurus
1.1.1.188
0.018
-
Hydrindantin
lung enzyme
Bos taurus
1.1.1.188
0.08
-
Phenylglyoxal
lung enzyme
Bos taurus
1.1.1.188
0.1
-
Prostaglandin D2
lung enzyme
Bos taurus
1.1.1.188
0.125
-
4-nitrobenzaldehyde
lung enzyme
Bos taurus
1.1.1.188
0.25
-
Prostaglandin A2
lung enzyme
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.188
30500
-
SDS-PAGE, sedimentation equilibrium and gel filtration
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.188
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.188
lung
two different active sites
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.188
0.15
-
prostaglandin F2alpha as product
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH
-
389460
Bos taurus
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
-
1.1.1.188
9,10-phenanthrenequinone + NADPH
-
389460
Bos taurus
?
-
-
-
?
1.1.1.188
hydrindantin + NADPH
-
389460
Bos taurus
?
-
-
-
-
1.1.1.188
phenylglyoxal + NADPH
-
389460
Bos taurus
?
-
-
-
-
1.1.1.188
prostaglandin A2 + NADPH
-
389460
Bos taurus
?
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
-
389460
Bos taurus
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.188
37
-
assay at
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.188
6
7
-
Bos taurus