BRENDA - Enzyme Database show

Expression of bovine lung prostaglandin F synthase in Escherichia coli

Watanabe, K.; Fujii, Y.; Ohkubo, H.; Kuramitsu, S.; Kagamiyama, H.; Nakanishi, S.; Hayaishi, O.; Biochem. Biophys. Res. Commun. 181, 272-278 (1991)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.188
expression in Escherichia coli
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.188
0.0001
-
9,10-phenanthrenequinone
expressed lung enzyme
Bos taurus
1.1.1.188
0.008
-
prostaglandin H2
expressed lung enzyme
Bos taurus
1.1.1.188
0.1
-
Prostaglandin D2
expressed lung enzyme
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.188
36000
-
gel filtration; SDS-PAGE
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.188
Bos taurus
-
two isozymes, lung-type and liver-type
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.188
partial
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.188
lung
two different active sites
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.188
0.115
-
4-nitrobenzaldehyde reductase activity
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH
-
389455
Bos taurus
(5Z,13E)-(15S)-9alpha,11beta,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
1.1.1.188
9,10-phenanthrenequinone + NADPH
-
389455
Bos taurus
?
-
-
-
-
1.1.1.188
additional information
two different active sites, one for the endoperoxide group of prostaglandin H2 and the other one for the keto group of prostaglandin D2 and other carbonyl compounds
389455
Bos taurus
?
-
-
-
-
1.1.1.188
prostaglandin H2 + NADPH
-
389455
Bos taurus
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.188
monomer
1 * 36000, gel filtration; 1 * 36000, SDS-PAGE
Bos taurus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADPH
-
Bos taurus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.188
expression in Escherichia coli
Bos taurus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADPH
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.188
0.0001
-
9,10-phenanthrenequinone
expressed lung enzyme
Bos taurus
1.1.1.188
0.008
-
prostaglandin H2
expressed lung enzyme
Bos taurus
1.1.1.188
0.1
-
Prostaglandin D2
expressed lung enzyme
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.188
36000
-
gel filtration; SDS-PAGE
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.188
partial
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.188
lung
two different active sites
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.188
0.115
-
4-nitrobenzaldehyde reductase activity
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH
-
389455
Bos taurus
(5Z,13E)-(15S)-9alpha,11beta,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
1.1.1.188
9,10-phenanthrenequinone + NADPH
-
389455
Bos taurus
?
-
-
-
-
1.1.1.188
additional information
two different active sites, one for the endoperoxide group of prostaglandin H2 and the other one for the keto group of prostaglandin D2 and other carbonyl compounds
389455
Bos taurus
?
-
-
-
-
1.1.1.188
prostaglandin H2 + NADPH
-
389455
Bos taurus
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.188
monomer
1 * 36000, gel filtration; 1 * 36000, SDS-PAGE
Bos taurus