BRENDA - Enzyme Database show

Purification and characterization of prostaglandin F synthase from bovine liver

Chen, L.Y.; Watanabe, K.; Hayaishi, O.; Arch. Biochem. Biophys. 296, 17-26 (1992)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.188
chloride
stimulates the reduction of prostaglandin D2 and carbonyl compounds, but had no effect on prostaglandin H2 reduction
Bos taurus
1.1.1.188
NaBr
stimulates prostaglandin D2 11-ketoreductase activity
Bos taurus
1.1.1.188
NaCl
stimulates prostaglandin D2 11-ketoreductase activity
Bos taurus
1.1.1.188
NaI
stimulates prostaglandin D2 11-ketoreductase activity
Bos taurus
1.1.1.188
piroxicam
-
Bos taurus
1.1.1.188
warfarin
-
Bos taurus
General Stability
EC Number
General Stability
Organism
1.1.1.188
activity is reduced less than 20% after freezing and thawing several times
Bos taurus
1.1.1.188
several freezing and thawing cycles reduce the activity of stored purified liver-type isozyme by 50%
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.188
4-nitrobenzaldehyde
competitive inhibition of PGD2 but not of PGH2 reduction
Bos taurus
1.1.1.188
9,10-phenanthrenequinone
competitive inhibition of PGD2 but not of PGH2 reduction; competitive inhibitor of the reduction of prostaglandin D2, not of prostaglandin H2
Bos taurus
1.1.1.188
Ag+
IC50 = 0.005 mM
Bos taurus
1.1.1.188
AgNO3
at 0.05 mM 87-100% inhibition, depending on the substrate
Bos taurus
1.1.1.188
Cd2+
IC50 = 0.95 mM
Bos taurus
1.1.1.188
CdSO4
at 5 mM 91-100% inhibition, depending on the substrate
Bos taurus
1.1.1.188
Cu2+
IC50 = 0.4 mM
Bos taurus
1.1.1.188
CuSO4
at 5 mM 93-96% inhibition, depending on the substrate; the liver-type isozyme is 2fold less sensitive than the lung-type isozyme
Bos taurus
1.1.1.188
HgCl2
at 1 mM 94-100% inhibition, depending on the substrate; IC50 = 0.3 mM, the liver-type isozyme is 2fold less sensitive than the lung-type isozyme
Bos taurus
1.1.1.188
NaF
-
Bos taurus
1.1.1.188
Zn2+
IC50 = 1.4 mM
Bos taurus
1.1.1.188
ZnSO4
at 5 mM 71-100% inhibition, depending on the substrate
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.188
0.002
-
9,10-phenanthrenequinone
; 37C, liver-type isozyme
Bos taurus
1.1.1.188
0.0031
-
Hydrindantin
; 37C, liver-type isozyme
Bos taurus
1.1.1.188
0.0056
-
Prostaglandin D3
; 37C, liver-type isozyme
Bos taurus
1.1.1.188
0.007
-
prostaglandin D1
; 37C, liver-type isozyme
Bos taurus
1.1.1.188
0.01
-
Prostaglandin D2
; 37C, liver-type isozyme
Bos taurus
1.1.1.188
0.0119
-
4-Nitroacetophenone
37C, liver-type isozyme
Bos taurus
1.1.1.188
0.0119
-
p-nitroacetophenone
-
Bos taurus
1.1.1.188
0.0143
-
4-nitrobenzaldehyde
; 37C, liver-type isozyme
Bos taurus
1.1.1.188
0.0167
-
menadione
; 37C, liver-type isozyme
Bos taurus
1.1.1.188
0.0175
-
5beta-dihydrotestosterone
; 37C, liver-type isozyme
Bos taurus
1.1.1.188
0.02
-
Phenylglyoxal
; 37C, liver-type isozyme
Bos taurus
1.1.1.188
0.025
-
prostaglandin H2
; 37C, liver-type isozyme
Bos taurus
1.1.1.188
0.053
-
cyclohexanone
; 37C, liver-type isozyme
Bos taurus
1.1.1.188
0.12
-
Prostaglandin D2
37C, lung-type isozyme
Bos taurus
1.1.1.188
0.143
-
DL-glyceraldehyde
; 37C, liver-type isozyme
Bos taurus
1.1.1.188
135
-
D-xylose
-
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.188
cytosol
liver-type isozyme
Bos taurus
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.188
CaCl2
activation
Bos taurus
1.1.1.188
Cl-
the liver-type isozyme is 3fold more activated than the lung-type isozyme
Bos taurus
1.1.1.188
MgCl2
activation
Bos taurus
1.1.1.188
NaBr
activation
Bos taurus
1.1.1.188
NaCl
activation
Bos taurus
1.1.1.188
NaI
activation
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.188
36000
-
liver-type isozyme, gel filtration; SDS-PAGE
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.188
Bos taurus
-
; liver-type and lung-type isozymes
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.188
native liver-type isozyme from liver, 342fold to homogeneity by ammonium sulfate fractionation, anion exchange and adsorption chromatography
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.188
liver
liver-type isozyme; two different active sites
Bos taurus
-
1.1.1.188
lung
lung-type isozyme
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.1.1.188
additional information
-
-
Bos taurus
1.1.1.188
0.003
-
prostaglandin H2 as substrate; purified liver-type isozyme, substrate prostaglandin H2
Bos taurus
1.1.1.188
0.008
-
purified liver-type isozyme, substrate prostaglandin I2
Bos taurus
1.1.1.188
0.046
-
prostaglandin D1 as substrate; purified liver-type isozyme, substrate prostaglandin D1
Bos taurus
1.1.1.188
0.065
-
xylose as substrate
Bos taurus
1.1.1.188
0.089
-
prostaglandin D3 as substrate; purified liver-type isozyme, substrate prostaglandin D3
Bos taurus
1.1.1.188
0.095
-
prostaglandin D2 as substrate; purified liver-type isozyme, substrate prostaglandin D2
Bos taurus
1.1.1.188
0.13
-
5beta-dihydrotestosterone as substrate
Bos taurus
1.1.1.188
0.159
-
cyclohexanone as substrate
Bos taurus
1.1.1.188
0.167
-
menadione and hydrindantin as substrate
Bos taurus
1.1.1.188
0.174
-
4-nitroacetophenone as substrate
Bos taurus
1.1.1.188
0.206
-
DL-glyceraldehyde as substrate
Bos taurus
1.1.1.188
0.207
-
phenylglyoxal as substrate
Bos taurus
1.1.1.188
0.31
-
4-nitrobenzaldehyde as substrate; purified liver-type isozyme, substrate 4-nitrobenzaldehyde
Bos taurus
1.1.1.188
0.339
-
9,10-phenanthrenequinone as substrate; purified liver-type isozyme, substrate 9,10-phenanthrenequinone
Bos taurus
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.188
-80C, concentration 1.5 mg per ml 10 mM potassium phosphate buffer pH 7.0, stable for several months
Bos taurus
1.1.1.188
-80C, purified liver-type isozyme at 1.5 mg/ml in 10 mM potassium phosphate, pH 7.0, stable for several months, several freezing and thawing cycles reduce the activity by 50%
Bos taurus
1.1.1.188
25C, concentration 1.5 mg per ml 10 mM potassium phosphate buffer pH 7.0, 80-90% activity, 1 month
Bos taurus
1.1.1.188
4-23C, purified liver-type isozyme, 1 month, 80-90% remaining activity
Bos taurus
1.1.1.188
4C, concentration 1.5 mg per ml 10 mM potassium phosphate buffer pH 7.0, 80-90% activity, 1 month
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH
-
389454
Bos taurus
(5Z,13E)-(15S)-9alpha,11beta,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
1.1.1.188
4-nitroacetophenone + NADPH
high activity
389454
Bos taurus
?
-
-
-
?
1.1.1.188
4-nitrobenzaldehyde + NADPH
-
389454
Bos taurus
4-nitrobenzyl alcohol + NADP+
-
-
-
?
1.1.1.188
4-nitrobenzaldehyde + NADPH
high activity
389454
Bos taurus
4-nitrobenzalcohol + NADP+
-
-
-
?
1.1.1.188
5beta-dihydrotestosterone + NADPH
-
389454
Bos taurus
?
-
-
-
?
1.1.1.188
9,10-phenanthrenequinone + NADPH
-
389454
Bos taurus
?
-
-
-
?
1.1.1.188
9,10-phenanthrenequinone + NADPH
best substrate
389454
Bos taurus
?
-
-
-
?
1.1.1.188
cyclohexanone + NADPH
-
389454
Bos taurus
cyclohexanol + NADP+
-
-
-
?
1.1.1.188
cyclohexanone + NADPH
high activity
389454
Bos taurus
cyclohexanol + NADP+
-
-
-
?
1.1.1.188
D-xylose + NADPH
-
389454
Bos taurus
?
-
-
-
?
1.1.1.188
daunorubicin + NADPH
high activity
389454
Bos taurus
?
-
-
-
?
1.1.1.188
DL-glyceraldehyde + NADPH
-
389454
Bos taurus
DL-glycerol + NADP+
-
-
-
?
1.1.1.188
DL-glyceraldehyde + NADPH
-
389454
Bos taurus
glyceral + NADP+
-
-
-
?
1.1.1.188
duroquinone + NADPH
-
389454
Bos taurus
?
-
-
-
?
1.1.1.188
hydrindantin + NADPH
-
389454
Bos taurus
?
-
-
-
?
1.1.1.188
hydrindantin + NADPH
high activity
389454
Bos taurus
?
-
-
-
?
1.1.1.188
menadione + NADPH
-
389454
Bos taurus
?
-
-
-
?
1.1.1.188
menadione + NADPH
high activity
389454
Bos taurus
?
-
-
-
?
1.1.1.188
additional information
two different active sites, one for the endoperoxide group of prostaglandin H2 and the other one for the keto group of prostaglandin D2 and other carbonyl compounds
389454
Bos taurus
?
-
-
-
-
1.1.1.188
additional information
the liver-type isozyme possesses two catalytic centres and a broad substrate specificity, D-xylose is a poor substrate, no activity with prostaglandin A1, prostaglandin B2, prostaglandin E2, 4-carboxybenzaldehyde, testosterone, 2,6-dichloroindophenol, D-glucose, D-glucuronic acid, and potassium ferricyanide, overview, the enzyme also exhibits aldose reductase, EC 1.1.1.21, and alcohol dehydrogenase, EC 1.1.1.1, activities, respectively
389454
Bos taurus
?
-
-
-
-
1.1.1.188
n-butyraldehyde + NADPH
-
389454
Bos taurus
n-butanol + NADP+
-
-
-
?
1.1.1.188
p-nitroacetophenone + NADPH
-
389454
Bos taurus
?
-
-
-
?
1.1.1.188
phenylglyoxal + NADPH
-
389454
Bos taurus
?
-
-
-
?
1.1.1.188
phenylglyoxal + NADPH
high activity
389454
Bos taurus
?
-
-
-
?
1.1.1.188
prostaglandin D1 + NADPH
-
389454
Bos taurus
?
-
-
-
?
1.1.1.188
prostaglandin D2 + NADPH
-
389454
Bos taurus
9alpha,11beta-prostaglandin F2 + NADP+
-
-
-
?
1.1.1.188
prostaglandin D3 + NADPH
-
389454
Bos taurus
?
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
-
389454
Bos taurus
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
-
389454
Bos taurus
9alpha,11alpha-prostaglandin F2alpha + NADP+
-
-
-
?
1.1.1.188
prostaglandin I2 + NADPH
-
389454
Bos taurus
?
-
-
-
?
1.1.1.188
ubiquinone-10 + NADPH
-
389454
Bos taurus
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.188
monomer
1 * 36000, SDS-PAGE; 1 x 36000, liver-type isozyme, SDS-PAGE
Bos taurus
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.1.1.188
100
-
5 min, inactivation; no reduction of prostaglandin D2 11-reductase after 5 min
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.188
6
7
-
Bos taurus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADPH
; highly preferred cofactor
Bos taurus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.188
0.0009
-
9,10-phenanthrenequinone
37C, liver-type isozyme, versus prostaglandin D2
Bos taurus
1.1.1.188
0.005
-
AgNO3
prostaglandin D2 11-ketoreductase activity
Bos taurus
1.1.1.188
0.023
-
4-nitrobenzaldehyde
37C, liver-type isozyme, versus prostaglandin D2
Bos taurus
1.1.1.188
0.3
-
HgCl2
prostaglandin D2 11-ketoreductase activity
Bos taurus
1.1.1.188
0.4
-
CuSO4
prostaglandin D2 11-ketoreductase activity
Bos taurus
1.1.1.188
0.95
-
CdSO4
prostaglandin D2 11-ketoreductase activity
Bos taurus
1.1.1.188
1.4
-
ZnSO4
prostaglandin D2 11-ketoreductase activity
Bos taurus
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
1.1.1.188
0.005
-
IC50 = 0.005 mM
Bos taurus
Ag+
1.1.1.188
0.3
-
IC50 = 0.3 mM, the liver-type isozyme is 2fold less sensitive than the lung-type isozyme
Bos taurus
HgCl2
1.1.1.188
0.4
-
IC50 = 0.4 mM
Bos taurus
Cu2+
1.1.1.188
0.95
-
IC50 = 0.95 mM
Bos taurus
Cd2+
1.1.1.188
1.4
-
IC50 = 1.4 mM
Bos taurus
Zn2+
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.188
chloride
stimulates the reduction of prostaglandin D2 and carbonyl compounds, but had no effect on prostaglandin H2 reduction
Bos taurus
1.1.1.188
NaBr
stimulates prostaglandin D2 11-ketoreductase activity
Bos taurus
1.1.1.188
NaCl
stimulates prostaglandin D2 11-ketoreductase activity
Bos taurus
1.1.1.188
NaI
stimulates prostaglandin D2 11-ketoreductase activity
Bos taurus
1.1.1.188
piroxicam
-
Bos taurus
1.1.1.188
warfarin
-
Bos taurus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADPH
; highly preferred cofactor
Bos taurus
General Stability (protein specific)
EC Number
General Stability
Organism
1.1.1.188
activity is reduced less than 20% after freezing and thawing several times
Bos taurus
1.1.1.188
several freezing and thawing cycles reduce the activity of stored purified liver-type isozyme by 50%
Bos taurus
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
1.1.1.188
0.005
-
IC50 = 0.005 mM
Bos taurus
Ag+
1.1.1.188
0.3
-
IC50 = 0.3 mM, the liver-type isozyme is 2fold less sensitive than the lung-type isozyme
Bos taurus
HgCl2
1.1.1.188
0.4
-
IC50 = 0.4 mM
Bos taurus
Cu2+
1.1.1.188
0.95
-
IC50 = 0.95 mM
Bos taurus
Cd2+
1.1.1.188
1.4
-
IC50 = 1.4 mM
Bos taurus
Zn2+
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.188
4-nitrobenzaldehyde
competitive inhibition of PGD2 but not of PGH2 reduction
Bos taurus
1.1.1.188
9,10-phenanthrenequinone
competitive inhibition of PGD2 but not of PGH2 reduction; competitive inhibitor of the reduction of prostaglandin D2, not of prostaglandin H2
Bos taurus
1.1.1.188
Ag+
IC50 = 0.005 mM
Bos taurus
1.1.1.188
AgNO3
at 0.05 mM 87-100% inhibition, depending on the substrate
Bos taurus
1.1.1.188
Cd2+
IC50 = 0.95 mM
Bos taurus
1.1.1.188
CdSO4
at 5 mM 91-100% inhibition, depending on the substrate
Bos taurus
1.1.1.188
Cu2+
IC50 = 0.4 mM
Bos taurus
1.1.1.188
CuSO4
at 5 mM 93-96% inhibition, depending on the substrate; the liver-type isozyme is 2fold less sensitive than the lung-type isozyme
Bos taurus
1.1.1.188
HgCl2
at 1 mM 94-100% inhibition, depending on the substrate; IC50 = 0.3 mM, the liver-type isozyme is 2fold less sensitive than the lung-type isozyme
Bos taurus
1.1.1.188
NaF
-
Bos taurus
1.1.1.188
Zn2+
IC50 = 1.4 mM
Bos taurus
1.1.1.188
ZnSO4
at 5 mM 71-100% inhibition, depending on the substrate
Bos taurus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.188
0.0009
-
9,10-phenanthrenequinone
37C, liver-type isozyme, versus prostaglandin D2
Bos taurus
1.1.1.188
0.005
-
AgNO3
prostaglandin D2 11-ketoreductase activity
Bos taurus
1.1.1.188
0.023
-
4-nitrobenzaldehyde
37C, liver-type isozyme, versus prostaglandin D2
Bos taurus
1.1.1.188
0.3
-
HgCl2
prostaglandin D2 11-ketoreductase activity
Bos taurus
1.1.1.188
0.4
-
CuSO4
prostaglandin D2 11-ketoreductase activity
Bos taurus
1.1.1.188
0.95
-
CdSO4
prostaglandin D2 11-ketoreductase activity
Bos taurus
1.1.1.188
1.4
-
ZnSO4
prostaglandin D2 11-ketoreductase activity
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.188
0.002
-
9,10-phenanthrenequinone
; 37C, liver-type isozyme
Bos taurus
1.1.1.188
0.0031
-
Hydrindantin
; 37C, liver-type isozyme
Bos taurus
1.1.1.188
0.0056
-
Prostaglandin D3
; 37C, liver-type isozyme
Bos taurus
1.1.1.188
0.007
-
prostaglandin D1
; 37C, liver-type isozyme
Bos taurus
1.1.1.188
0.01
-
Prostaglandin D2
; 37C, liver-type isozyme
Bos taurus
1.1.1.188
0.0119
-
4-Nitroacetophenone
37C, liver-type isozyme
Bos taurus
1.1.1.188
0.0119
-
p-nitroacetophenone
-
Bos taurus
1.1.1.188
0.0143
-
4-nitrobenzaldehyde
; 37C, liver-type isozyme
Bos taurus
1.1.1.188
0.0167
-
menadione
; 37C, liver-type isozyme
Bos taurus
1.1.1.188
0.0175
-
5beta-dihydrotestosterone
; 37C, liver-type isozyme
Bos taurus
1.1.1.188
0.02
-
Phenylglyoxal
; 37C, liver-type isozyme
Bos taurus
1.1.1.188
0.025
-
prostaglandin H2
; 37C, liver-type isozyme
Bos taurus
1.1.1.188
0.053
-
cyclohexanone
; 37C, liver-type isozyme
Bos taurus
1.1.1.188
0.12
-
Prostaglandin D2
37C, lung-type isozyme
Bos taurus
1.1.1.188
0.143
-
DL-glyceraldehyde
; 37C, liver-type isozyme
Bos taurus
1.1.1.188
135
-
D-xylose
-
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.188
cytosol
liver-type isozyme
Bos taurus
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.188
CaCl2
activation
Bos taurus
1.1.1.188
Cl-
the liver-type isozyme is 3fold more activated than the lung-type isozyme
Bos taurus
1.1.1.188
MgCl2
activation
Bos taurus
1.1.1.188
NaBr
activation
Bos taurus
1.1.1.188
NaCl
activation
Bos taurus
1.1.1.188
NaI
activation
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.188
36000
-
liver-type isozyme, gel filtration; SDS-PAGE
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.188
native liver-type isozyme from liver, 342fold to homogeneity by ammonium sulfate fractionation, anion exchange and adsorption chromatography
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.188
liver
liver-type isozyme; two different active sites
Bos taurus
-
1.1.1.188
lung
lung-type isozyme
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.1.1.188
additional information
-
-
Bos taurus
1.1.1.188
0.003
-
prostaglandin H2 as substrate; purified liver-type isozyme, substrate prostaglandin H2
Bos taurus
1.1.1.188
0.008
-
purified liver-type isozyme, substrate prostaglandin I2
Bos taurus
1.1.1.188
0.046
-
prostaglandin D1 as substrate; purified liver-type isozyme, substrate prostaglandin D1
Bos taurus
1.1.1.188
0.065
-
xylose as substrate
Bos taurus
1.1.1.188
0.089
-
prostaglandin D3 as substrate; purified liver-type isozyme, substrate prostaglandin D3
Bos taurus
1.1.1.188
0.095
-
prostaglandin D2 as substrate; purified liver-type isozyme, substrate prostaglandin D2
Bos taurus
1.1.1.188
0.13
-
5beta-dihydrotestosterone as substrate
Bos taurus
1.1.1.188
0.159
-
cyclohexanone as substrate
Bos taurus
1.1.1.188
0.167
-
menadione and hydrindantin as substrate
Bos taurus
1.1.1.188
0.174
-
4-nitroacetophenone as substrate
Bos taurus
1.1.1.188
0.206
-
DL-glyceraldehyde as substrate
Bos taurus
1.1.1.188
0.207
-
phenylglyoxal as substrate
Bos taurus
1.1.1.188
0.31
-
4-nitrobenzaldehyde as substrate; purified liver-type isozyme, substrate 4-nitrobenzaldehyde
Bos taurus
1.1.1.188
0.339
-
9,10-phenanthrenequinone as substrate; purified liver-type isozyme, substrate 9,10-phenanthrenequinone
Bos taurus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.188
-80C, concentration 1.5 mg per ml 10 mM potassium phosphate buffer pH 7.0, stable for several months
Bos taurus
1.1.1.188
-80C, purified liver-type isozyme at 1.5 mg/ml in 10 mM potassium phosphate, pH 7.0, stable for several months, several freezing and thawing cycles reduce the activity by 50%
Bos taurus
1.1.1.188
25C, concentration 1.5 mg per ml 10 mM potassium phosphate buffer pH 7.0, 80-90% activity, 1 month
Bos taurus
1.1.1.188
4-23C, purified liver-type isozyme, 1 month, 80-90% remaining activity
Bos taurus
1.1.1.188
4C, concentration 1.5 mg per ml 10 mM potassium phosphate buffer pH 7.0, 80-90% activity, 1 month
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH
-
389454
Bos taurus
(5Z,13E)-(15S)-9alpha,11beta,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
1.1.1.188
4-nitroacetophenone + NADPH
high activity
389454
Bos taurus
?
-
-
-
?
1.1.1.188
4-nitrobenzaldehyde + NADPH
-
389454
Bos taurus
4-nitrobenzyl alcohol + NADP+
-
-
-
?
1.1.1.188
4-nitrobenzaldehyde + NADPH
high activity
389454
Bos taurus
4-nitrobenzalcohol + NADP+
-
-
-
?
1.1.1.188
5beta-dihydrotestosterone + NADPH
-
389454
Bos taurus
?
-
-
-
?
1.1.1.188
9,10-phenanthrenequinone + NADPH
-
389454
Bos taurus
?
-
-
-
?
1.1.1.188
9,10-phenanthrenequinone + NADPH
best substrate
389454
Bos taurus
?
-
-
-
?
1.1.1.188
cyclohexanone + NADPH
-
389454
Bos taurus
cyclohexanol + NADP+
-
-
-
?
1.1.1.188
cyclohexanone + NADPH
high activity
389454
Bos taurus
cyclohexanol + NADP+
-
-
-
?
1.1.1.188
D-xylose + NADPH
-
389454
Bos taurus
?
-
-
-
?
1.1.1.188
daunorubicin + NADPH
high activity
389454
Bos taurus
?
-
-
-
?
1.1.1.188
DL-glyceraldehyde + NADPH
-
389454
Bos taurus
DL-glycerol + NADP+
-
-
-
?
1.1.1.188
DL-glyceraldehyde + NADPH
-
389454
Bos taurus
glyceral + NADP+
-
-
-
?
1.1.1.188
duroquinone + NADPH
-
389454
Bos taurus
?
-
-
-
?
1.1.1.188
hydrindantin + NADPH
-
389454
Bos taurus
?
-
-
-
?
1.1.1.188
hydrindantin + NADPH
high activity
389454
Bos taurus
?
-
-
-
?
1.1.1.188
menadione + NADPH
-
389454
Bos taurus
?
-
-
-
?
1.1.1.188
menadione + NADPH
high activity
389454
Bos taurus
?
-
-
-
?
1.1.1.188
additional information
two different active sites, one for the endoperoxide group of prostaglandin H2 and the other one for the keto group of prostaglandin D2 and other carbonyl compounds
389454
Bos taurus
?
-
-
-
-
1.1.1.188
additional information
the liver-type isozyme possesses two catalytic centres and a broad substrate specificity, D-xylose is a poor substrate, no activity with prostaglandin A1, prostaglandin B2, prostaglandin E2, 4-carboxybenzaldehyde, testosterone, 2,6-dichloroindophenol, D-glucose, D-glucuronic acid, and potassium ferricyanide, overview, the enzyme also exhibits aldose reductase, EC 1.1.1.21, and alcohol dehydrogenase, EC 1.1.1.1, activities, respectively
389454
Bos taurus
?
-
-
-
-
1.1.1.188
n-butyraldehyde + NADPH
-
389454
Bos taurus
n-butanol + NADP+
-
-
-
?
1.1.1.188
p-nitroacetophenone + NADPH
-
389454
Bos taurus
?
-
-
-
?
1.1.1.188
phenylglyoxal + NADPH
-
389454
Bos taurus
?
-
-
-
?
1.1.1.188
phenylglyoxal + NADPH
high activity
389454
Bos taurus
?
-
-
-
?
1.1.1.188
prostaglandin D1 + NADPH
-
389454
Bos taurus
?
-
-
-
?
1.1.1.188
prostaglandin D2 + NADPH
-
389454
Bos taurus
9alpha,11beta-prostaglandin F2 + NADP+
-
-
-
?
1.1.1.188
prostaglandin D3 + NADPH
-
389454
Bos taurus
?
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
-
389454
Bos taurus
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
-
389454
Bos taurus
9alpha,11alpha-prostaglandin F2alpha + NADP+
-
-
-
?
1.1.1.188
prostaglandin I2 + NADPH
-
389454
Bos taurus
?
-
-
-
?
1.1.1.188
ubiquinone-10 + NADPH
-
389454
Bos taurus
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.188
monomer
1 * 36000, SDS-PAGE; 1 x 36000, liver-type isozyme, SDS-PAGE
Bos taurus
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.1.1.188
100
-
5 min, inactivation; no reduction of prostaglandin D2 11-reductase after 5 min
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.188
6
7
-
Bos taurus