BRENDA - Enzyme Database show

A lung type prostaglandin F synthase is expressed in bovine liver: cDNA sequence and expression in E. coli

Kuchinke, W.; Barski, O.; Watanabe, K.; Hayaishi, O.; Biochem. Biophys. Res. Commun. 183, 1238-1246 (1992)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.188
expression in Escherichia coli
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.188
0.00013
-
9,10-phenanthrenequinone
lung enzyme
Bos taurus
1.1.1.188
0.0008
-
4-nitrobenzaldehyde
liver enzyme
Bos taurus
1.1.1.188
0.001
-
9,10-phenanthrenequinone
liver enzyme
Bos taurus
1.1.1.188
0.0083
-
4-nitrobenzaldehyde
lung enzyme
Bos taurus
1.1.1.188
0.013
-
menadione
lung enzyme
Bos taurus
1.1.1.188
0.1
-
menadione
liver enzyme
Bos taurus
1.1.1.188
0.1
-
Prostaglandin D2
lung enzyme
Bos taurus
1.1.1.188
0.2
-
Prostaglandin D2
liver enzyme
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.188
36000
-
gel filtration
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.188
Bos taurus
-
two isozymes, lung-type and liver-type
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.188
-
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.188
liver
-
Bos taurus
-
1.1.1.188
lung
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.188
0.034
-
-
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH
-
389453
Bos taurus
(5Z,13E)-(15S)-9alpha,11beta,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
1.1.1.188
4-nitrobenzaldehyde + NADPH
-
389453
Bos taurus
4-nitrobenzyl alcohol + NADP+
-
-
-
?
1.1.1.188
9,10-phenanthrenequinone + NADPH
-
389453
Bos taurus
?
-
-
-
?
1.1.1.188
menadione + NADPH
-
389453
Bos taurus
?
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
-
389453
Bos taurus
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.188
monomer
1 * 36000, gel filtration
Bos taurus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADPH
-
Bos taurus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.188
expression in Escherichia coli
Bos taurus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADPH
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.188
0.00013
-
9,10-phenanthrenequinone
lung enzyme
Bos taurus
1.1.1.188
0.0008
-
4-nitrobenzaldehyde
liver enzyme
Bos taurus
1.1.1.188
0.001
-
9,10-phenanthrenequinone
liver enzyme
Bos taurus
1.1.1.188
0.0083
-
4-nitrobenzaldehyde
lung enzyme
Bos taurus
1.1.1.188
0.013
-
menadione
lung enzyme
Bos taurus
1.1.1.188
0.1
-
menadione
liver enzyme
Bos taurus
1.1.1.188
0.1
-
Prostaglandin D2
lung enzyme
Bos taurus
1.1.1.188
0.2
-
Prostaglandin D2
liver enzyme
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.188
36000
-
gel filtration
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.188
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.188
liver
-
Bos taurus
-
1.1.1.188
lung
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.188
0.034
-
-
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH
-
389453
Bos taurus
(5Z,13E)-(15S)-9alpha,11beta,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
1.1.1.188
4-nitrobenzaldehyde + NADPH
-
389453
Bos taurus
4-nitrobenzyl alcohol + NADP+
-
-
-
?
1.1.1.188
9,10-phenanthrenequinone + NADPH
-
389453
Bos taurus
?
-
-
-
?
1.1.1.188
menadione + NADPH
-
389453
Bos taurus
?
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
-
389453
Bos taurus
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.188
monomer
1 * 36000, gel filtration
Bos taurus