BRENDA - Enzyme Database show

Kinetic mechanism of ketoreductase activity of prostaglandin F synthase from bovine lung

Barski, O.A.; Watanabe, K.; FEBS Lett. 320, 107-110 (1993)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.188
expression in Escherichia coli
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.188
0.0034
-
NADPH
-
Bos taurus
1.1.1.188
0.174
-
Prostaglandin D2
-
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.188
Bos taurus
-
two isozymes, lung-type and liver-type
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.188
lung
two different active sites
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH
-
389452
Bos taurus
(5Z,13E)-(15S)-9alpha,11beta,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
1.1.1.188
4-nitrobenzaldehyde + NADPH
-
389452
Bos taurus
4-nitrobenzyl alcohol + NADP+
-
-
-
?
1.1.1.188
9,10-phenanthrenequinone + NADPH
-
389452
Bos taurus
?
-
-
-
?
1.1.1.188
menadione + NADPH
-
389452
Bos taurus
?
-
-
-
?
1.1.1.188
additional information
two different active sites, one for the endoperoxide group of prostaglandin H2 and the other one for the keto group of prostaglandin D2 and other carbonyl compounds
389452
Bos taurus
?
-
-
-
-
1.1.1.188
prostaglandin H2 + NADPH
-
389452
Bos taurus
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.188
37
-
assay at
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.188
6.5
-
assay at
Bos taurus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADPH
-
Bos taurus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.188
expression in Escherichia coli
Bos taurus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADPH
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.188
0.0034
-
NADPH
-
Bos taurus
1.1.1.188
0.174
-
Prostaglandin D2
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.188
lung
two different active sites
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH
-
389452
Bos taurus
(5Z,13E)-(15S)-9alpha,11beta,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
1.1.1.188
4-nitrobenzaldehyde + NADPH
-
389452
Bos taurus
4-nitrobenzyl alcohol + NADP+
-
-
-
?
1.1.1.188
9,10-phenanthrenequinone + NADPH
-
389452
Bos taurus
?
-
-
-
?
1.1.1.188
menadione + NADPH
-
389452
Bos taurus
?
-
-
-
?
1.1.1.188
additional information
two different active sites, one for the endoperoxide group of prostaglandin H2 and the other one for the keto group of prostaglandin D2 and other carbonyl compounds
389452
Bos taurus
?
-
-
-
-
1.1.1.188
prostaglandin H2 + NADPH
-
389452
Bos taurus
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.188
37
-
assay at
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.188
6.5
-
assay at
Bos taurus