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Literature summary extracted from

  • Barski, O.A.; Watanabe, K.
    Kinetic mechanism of ketoreductase activity of prostaglandin F synthase from bovine lung (1993), FEBS Lett., 320, 107-110.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.188 expression in Escherichia coli Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.188 0.0034
-
 NADPH
-
 Bos taurus
1.1.1.188 0.174
-
 Prostaglandin D2
-
 Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.188 Bos taurus
-
 two isozymes, lung-type and liver-type
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.188 lung two different active sites Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.188 (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH
-
 Bos taurus (5Z,13E)-(15S)-9alpha,11beta,15-trihydroxyprosta-5,13-dienoate + NADP+
-
 ?
1.1.1.188 4-nitrobenzaldehyde + NADPH
-
 Bos taurus 4-nitrobenzyl alcohol + NADP+
-
 ?
1.1.1.188 9,10-phenanthrenequinone + NADPH
-
 Bos taurus ?
-
 ?
1.1.1.188 menadione + NADPH
-
 Bos taurus ?
-
 ?
1.1.1.188 additional information two different active sites, one for the endoperoxide group of prostaglandin H2 and the other one for the keto group of prostaglandin D2 and other carbonyl compounds Bos taurus ?
-
 ?
1.1.1.188 prostaglandin H2 + NADPH + H+
-
 Bos taurus (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.188 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.188 6.5
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.188 NADPH
-
 Bos taurus