BRENDA - Enzyme Database show

cDNA cloning, expression, and mutagenesis study of liver-type prostaglandin F synthase

Suzuki, T.; Fujii, Y.; Miyano, M.; Chen, L.Y.; Takahashi, T.; Watanabe, K.; J. Biol. Chem. 274, 241-248 (1999)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.188
expression in COS-7 cells
Bos taurus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.188
H170C
fully active
Bos taurus
1.1.1.188
K225S
2fold as active as wild-type
Bos taurus
1.1.1.188
N306Y
half as active as wild-type
Bos taurus
1.1.1.188
R223L
5fold as active as wild-type
Bos taurus
1.1.1.188
R27E
fully active
Bos taurus
1.1.1.188
R91Q
fully active
Bos taurus
1.1.1.188
S301R
2.5fold as active as wild-type
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.188
0.0007
-
9,10-phenanthrenequinone
native lung prostaglandin F synthase
Bos taurus
1.1.1.188
0.0011
-
9,10-phenanthrenequinone
expressed liver prostaglandin F synthase
Bos taurus
1.1.1.188
0.002
-
9,10-phenanthrenequinone
native liver prostaglandin F synthase
Bos taurus
1.1.1.188
0.01
-
Prostaglandin D2
native liver prostaglandin F synthase
Bos taurus
1.1.1.188
0.01
-
prostaglandin H2
native lung prostaglandin F synthase
Bos taurus
1.1.1.188
0.015
-
Prostaglandin D2
expressed liver prostaglandin F synthase
Bos taurus
1.1.1.188
0.025
-
prostaglandin H2
native and expressed liver prostaglandin F synthase
Bos taurus
1.1.1.188
0.12
-
Prostaglandin D2
native lung prostaglandin F synthase
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.188
36740
-
amino acid analysis, SDS-PAGE
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.188
Bos taurus
-
two isozymes, lung-type and liver-type
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.188
-
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.188
liver
-
Bos taurus
-
1.1.1.188
lung
-
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH
-
389449
Bos taurus
(5Z,13E)-(15S)-9alpha,11beta,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
1.1.1.188
9,10-phenanthrenequinone + NADPH
-
389449
Bos taurus
?
-
-
-
-
1.1.1.188
prostaglandin H2 + NADPH
-
389449
Bos taurus
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADPH
-
Bos taurus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.188
expression in COS-7 cells
Bos taurus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADPH
-
Bos taurus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.188
H170C
fully active
Bos taurus
1.1.1.188
K225S
2fold as active as wild-type
Bos taurus
1.1.1.188
N306Y
half as active as wild-type
Bos taurus
1.1.1.188
R223L
5fold as active as wild-type
Bos taurus
1.1.1.188
R27E
fully active
Bos taurus
1.1.1.188
R91Q
fully active
Bos taurus
1.1.1.188
S301R
2.5fold as active as wild-type
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.188
0.0007
-
9,10-phenanthrenequinone
native lung prostaglandin F synthase
Bos taurus
1.1.1.188
0.0011
-
9,10-phenanthrenequinone
expressed liver prostaglandin F synthase
Bos taurus
1.1.1.188
0.002
-
9,10-phenanthrenequinone
native liver prostaglandin F synthase
Bos taurus
1.1.1.188
0.01
-
Prostaglandin D2
native liver prostaglandin F synthase
Bos taurus
1.1.1.188
0.01
-
prostaglandin H2
native lung prostaglandin F synthase
Bos taurus
1.1.1.188
0.015
-
Prostaglandin D2
expressed liver prostaglandin F synthase
Bos taurus
1.1.1.188
0.025
-
prostaglandin H2
native and expressed liver prostaglandin F synthase
Bos taurus
1.1.1.188
0.12
-
Prostaglandin D2
native lung prostaglandin F synthase
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.188
36740
-
amino acid analysis, SDS-PAGE
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.188
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.188
liver
-
Bos taurus
-
1.1.1.188
lung
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH
-
389449
Bos taurus
(5Z,13E)-(15S)-9alpha,11beta,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
1.1.1.188
9,10-phenanthrenequinone + NADPH
-
389449
Bos taurus
?
-
-
-
-
1.1.1.188
prostaglandin H2 + NADPH
-
389449
Bos taurus
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?