BRENDA - Enzyme Database show

cDNA cloning, expression and characterization of human prostaglandin F synthase

Suzuki-Yamamoto, T.; Nishizawa, M.; Fukui, M.; Okuda-Ashitaka, E.; Nakajima, T.; Ito, S.; Watanabe, K.; FEBS Lett. 462, 335-340 (1999)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.188
expression in Escherichia coli
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.188
0.0008
-
9,10-phenanthrenequinone
-
Homo sapiens
1.1.1.188
0.001
-
5beta-Androstan-3alpha-ol-17-one
-
Homo sapiens
1.1.1.188
0.0031
-
5beta-androstan-3,17-dione
-
Homo sapiens
1.1.1.188
0.0034
-
Prostaglandin D2
-
Homo sapiens
1.1.1.188
0.0774
-
4-nitrobenzaldehyde
-
Homo sapiens
1.1.1.188
0.134
-
(5Z,13E)-(15S)-9alpha,11beta,15-trihydroxyprosta-5,13-dienoate
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.188
cytosol
-
Homo sapiens
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.188
36840
-
amino acid analysis
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.188
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.188
lung
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH
-
389448
Homo sapiens
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
1.1.1.188
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH
-
389448
Homo sapiens
(5Z,13E)-(15S)-9alpha,11beta,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
1.1.1.188
4-nitrobenzaldehyde + NADPH
-
389448
Homo sapiens
4-nitrobenzyl alcohol + NADP+
-
-
-
?
1.1.1.188
5beta-androstane-3,17-dione + NADPH
-
389448
Homo sapiens
5beta-androstan-3alpha-ol-17-one + NADP+
-
-
-
r
1.1.1.188
9,10-phenanthrenequinone + NADPH
-
389448
Homo sapiens
?
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
-
389448
Homo sapiens
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.188
8
9
reduction of prostaglandin D2
Homo sapiens
1.1.1.188
9
10
oxidation of 9alpha,11beta-prostaglandin F2
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADPH
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.188
expression in Escherichia coli
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADPH
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.188
0.0008
-
9,10-phenanthrenequinone
-
Homo sapiens
1.1.1.188
0.001
-
5beta-Androstan-3alpha-ol-17-one
-
Homo sapiens
1.1.1.188
0.0031
-
5beta-androstan-3,17-dione
-
Homo sapiens
1.1.1.188
0.0034
-
Prostaglandin D2
-
Homo sapiens
1.1.1.188
0.0774
-
4-nitrobenzaldehyde
-
Homo sapiens
1.1.1.188
0.134
-
(5Z,13E)-(15S)-9alpha,11beta,15-trihydroxyprosta-5,13-dienoate
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.188
cytosol
-
Homo sapiens
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.188
36840
-
amino acid analysis
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.188
lung
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH
-
389448
Homo sapiens
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
1.1.1.188
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH
-
389448
Homo sapiens
(5Z,13E)-(15S)-9alpha,11beta,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
1.1.1.188
4-nitrobenzaldehyde + NADPH
-
389448
Homo sapiens
4-nitrobenzyl alcohol + NADP+
-
-
-
?
1.1.1.188
5beta-androstane-3,17-dione + NADPH
-
389448
Homo sapiens
5beta-androstan-3alpha-ol-17-one + NADP+
-
-
-
r
1.1.1.188
9,10-phenanthrenequinone + NADPH
-
389448
Homo sapiens
?
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
-
389448
Homo sapiens
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.188
8
9
reduction of prostaglandin D2
Homo sapiens
1.1.1.188
9
10
oxidation of 9alpha,11beta-prostaglandin F2
Homo sapiens