BRENDA - Enzyme Database show

Identification of a novel prostaglandin F2.alpha. synthase in Trypanosoma brucei

Kubata, B.K.; Duszenko, M.; Kabututu, Z.; Rawer, M.; Szallies, A.; Fujimori, K.; Inui, T.; Nozaki, T.; Yamashita, K.; Horii, T.; Urade, Y.; Hayaishi, O.; J. Exp. Med. 192, 1327-1337 (2000)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.188
expression in Escherichia coli
Trypanosoma brucei
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.188
0.00042
-
9,10-phenanthrenequinone
-
Trypanosoma brucei
1.1.1.188
0.0008
-
4-nitrobenzaldehyde
-
Trypanosoma brucei
1.1.1.188
0.0013
-
prostaglandin H2
-
Trypanosoma brucei
1.1.1.188
0.0057
-
testosterone
-
Trypanosoma brucei
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.188
cytosol
-
Trypanosoma brucei
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.188
33000
-
SDS-PAGE
Trypanosoma brucei
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.188
Trypanosoma brucei
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.188
-
Trypanosoma brucei
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.1.1.188
0.86
-
-
Trypanosoma brucei
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
4-nitrobenzaldehyde + NADPH
-
389446
Trypanosoma brucei
4-nitrobenzyl alcohol + NADP+
-
-
-
?
1.1.1.188
9,10-phenanthrenequinone + NADPH
-
389446
Trypanosoma brucei
?
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
-
389446
Trypanosoma brucei
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
1.1.1.188
testosterone + NADPH
-
389446
Trypanosoma brucei
?
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.188
37
-
assay at
Trypanosoma brucei
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.1.1.188
25
40
-
Trypanosoma brucei
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.188
7
-
assay at
Trypanosoma brucei
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.188
6
9
-
Trypanosoma brucei
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADPH
-
Trypanosoma brucei
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.188
expression in Escherichia coli
Trypanosoma brucei
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADPH
-
Trypanosoma brucei
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.188
0.00042
-
9,10-phenanthrenequinone
-
Trypanosoma brucei
1.1.1.188
0.0008
-
4-nitrobenzaldehyde
-
Trypanosoma brucei
1.1.1.188
0.0013
-
prostaglandin H2
-
Trypanosoma brucei
1.1.1.188
0.0057
-
testosterone
-
Trypanosoma brucei
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.188
cytosol
-
Trypanosoma brucei
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.188
33000
-
SDS-PAGE
Trypanosoma brucei
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.188
-
Trypanosoma brucei
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.1.1.188
0.86
-
-
Trypanosoma brucei
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
4-nitrobenzaldehyde + NADPH
-
389446
Trypanosoma brucei
4-nitrobenzyl alcohol + NADP+
-
-
-
?
1.1.1.188
9,10-phenanthrenequinone + NADPH
-
389446
Trypanosoma brucei
?
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH
-
389446
Trypanosoma brucei
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
1.1.1.188
testosterone + NADPH
-
389446
Trypanosoma brucei
?
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.1.188
37
-
assay at
Trypanosoma brucei
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.1.1.188
25
40
-
Trypanosoma brucei
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.188
7
-
assay at
Trypanosoma brucei
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.188
6
9
-
Trypanosoma brucei