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Literature summary extracted from

  • Chase, T.
    Mannitol-1-phosphate dehydrogenase of Escherichia coli (1986), Biochem. J., 239, 435-443.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.17 1,10-phenanthroline weak inhibition Escherichia coli
1.1.1.17 2,2'-bipyridyl weak inhibition Escherichia coli
1.1.1.17 4,7-phenanthroline weak inhibition Escherichia coli
1.1.1.17 5,5'-dithiobis-(2-nitrobenzoate) complete inactivation at 0.24 mM Escherichia coli
1.1.1.17 diethyldithiocarbamate 95% inactivation, prevented by presence of 2-mercaptoethanol Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.17 intracellular
-
 Escherichia coli 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.17 Zn2+ 1.14 Zn atoms/monomer Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.17 22500
-
 2 * 22500, SDS-PAGE Escherichia coli
1.1.1.17 45500
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.17 D-mannitol 1-phosphate + NAD+ Escherichia coli
-
 D-fructose 6-phosphate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.17 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.17 to homogeneity, chromatography techniques Escherichia coli

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.17 cell culture
-
 Escherichia coli
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.17 502
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.1.1.17 4°C, 0.03 M imidazole-HCl, pH 7.3, several months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.17 D-mannitol 1-phosphate + NAD+
-
 Escherichia coli D-fructose 6-phosphate + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.17 dimer 2 * 22500, SDS-PAGE Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.17 NAD+
-
 Escherichia coli
1.1.1.17 NADH
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.17 8
-
 1,10-phenanthroline
-
 Escherichia coli
1.1.1.17 11
-
 2,2'-bipyridyl
-
 Escherichia coli
1.1.1.17 12
-
 4,7-phenanthroline
-
 Escherichia coli