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Literature summary extracted from

  • Furster, C.; Zhang, J.; Toll, A.
    Purification of a 3beta-hydroxy-D5-C27-steroid dehydrogenase from pig liver microsomes active in major and alternative pathways of bile acid biosynthesis (1996), J. Biol. Chem., 271, 20903-20907.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.145 0.003
-
 7alpha,27-dihydroxycholesterol
-
 Sus scrofa
1.1.1.145 0.0032
-
 7alpha-hydroxycholesterol
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.145 microsome
-
 Sus scrofa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.145 36000
-
 x * 36000, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.145 pregnenolone + NAD(P)+ Sus scrofa
-
 pregn-5-en-3,20-dione + NAD(P)H + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.145 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.145
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.145 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.145 0.0033
-
 7alpha,25-dihydroxycholesterol as substrate Sus scrofa
1.1.1.145 0.175
-
 3beta,7alpha-dihydroxy-5-cholestenoic acid as substrate Sus scrofa
1.1.1.145 0.838
-
 7alpha,27-dihydroxycholesterol as substrate Sus scrofa
1.1.1.145 1.489
-
 7alpha-hydroxycholesterol as substrate Sus scrofa
1.1.1.145 2.88
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.145 3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
-
 Sus scrofa 17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
 ?
1.1.1.145 3beta,7alpha-dihydroxy-5-cholestenoic acid + NAD+
-
 Sus scrofa 7alpha-hydroxy-3-oxo-5-cholestenoic acid + NADH
-
 ?
1.1.1.145 7alpha,25-dihydroxycholesterol + NAD+
-
 Sus scrofa 7alpha,25-dihydroxy-4-cholesten-3-one + NADH
-
 ?
1.1.1.145 7alpha,27-dihydroxycholesterol + NAD+
-
 Sus scrofa 7alpha,27-dihydroxy-4-cholesten-3-one + NADH
-
 ?
1.1.1.145 7alpha-hydroxycholesterol + NAD+
-
 Sus scrofa 7alpha-hydroxy-4-cholesten-3-one + NADH
-
 ?
1.1.1.145 pregnenolone + NAD(P)+
-
 Sus scrofa pregn-5-en-3,20-dione + NAD(P)H + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.145 ? x * 36000, SDS-PAGE Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.145 NAD+
-
 Sus scrofa