Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hiwatashi, A.; Hamamoto, I.; Ichikawa, Y.
    Purification and kinetic properties of 3beta-hydroxysteroid dehydrogenase from bovine adrenocortical microsomes (1985), J. Biochem., 98, 1519-1526.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.145 17alpha-hydroxypregnenolone competitive inhibition Bos taurus
1.1.1.145 diethyldicarbonate
-
Bos taurus
1.1.1.145 p-chloromercuribenzoate
-
Bos taurus
1.1.1.145 pregnenolone competitive inhibition Bos taurus
1.1.1.145 trilostane i.e. 4alpha,5-epoxy-3,17beta-dihydroxy-5alpha-androst-2-en-2-carbonitrile Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.145 0.002
-
pregnenolone
-
Bos taurus
1.1.1.145 0.0053
-
3beta,17alpha-dihydroxy-5-pregen-20-one
-
Bos taurus
1.1.1.145 0.0062 0.0066 NAD+
-
Bos taurus
1.1.1.145 4.9
-
NADP+
-
Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.145 microsome
-
Bos taurus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.145 41000
-
x * 41000, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.145 pregnenolone + NAD(P)+ Bos taurus
-
pregn-5-en-3,20-dione + NAD(P)H + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.145 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.145
-
Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.145 adrenal gland
-
Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.145 0.677
-
-
Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.1.1.145 -80°C, 0.1 M potassium phosphate buffer, pH 7.4, 10 mg protein/ml, at least 3 months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.145 3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
-
Bos taurus 17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
?
1.1.1.145 pregnenolone + NAD(P)+
-
Bos taurus pregn-5-en-3,20-dione + NAD(P)H + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.145 ? x * 41000, SDS-PAGE Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.145 7.8 8.5
-
Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.145 NAD+ cysteinyl and histidyl at NAD+-binding site Bos taurus
1.1.1.145 NADP+ low activity Bos taurus