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Literature summary extracted from

  • Melendy, T.; Ray, D.S.
    Novobiocin affinity purification of a mitochondrial type II topoisomerase from the trypanosomatid Crithidia fasciculata (1989), J. Biol. Chem., 264, 1870-1876.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.2.2 4'-(9-acridinylamino)methansulfon-m-anisidide
-
 Crithidia fasciculata
5.6.2.2 Berenil
-
 Crithidia fasciculata
5.6.2.2 coumermycin
-
 Crithidia fasciculata
5.6.2.2 ellipticines
-
 Crithidia fasciculata
5.6.2.2 Ethidium bromide
-
 Crithidia fasciculata
5.6.2.2 etoposide
-
 Crithidia fasciculata
5.6.2.2 NEM
-
 Crithidia fasciculata
5.6.2.2 novobiocin
-
 Crithidia fasciculata
5.6.2.2 Teniposide
-
 Crithidia fasciculata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.6.2.2 mitochondrion
-
 Crithidia fasciculata 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.2.2 K+ requires 0.08-0.15 M NaCl or KCl, in the presence of 20 mM Tris-HCl and 5 mM MgCl2 Crithidia fasciculata
5.6.2.2 Mg2+ required Crithidia fasciculata
5.6.2.2 Mg2+ effective at 0.1-8 mM MgCl2 Crithidia fasciculata
5.6.2.2 NaCl requires 0.08-0.15 M NaCl or KCl, in the presence of 20 mM Tris-HCl and 5 mM MgCl2 Crithidia fasciculata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.2.2 132000
-
 2 * 132000, SDS-PAGE Crithidia fasciculata
5.6.2.2 264000
-
 gel filtration, glycerol density gradient sedimentation Crithidia fasciculata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.2.2 additional information Crithidia fasciculata localization of the enzyme within the single mitochondrion suggests an important role of the enzyme in kinetoplast DNA replication ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.2 Crithidia fasciculata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.2.2 novobiocin affinity purification Crithidia fasciculata

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.6.2.2 additional information
-
-
 Crithidia fasciculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.2 additional information localization of the enzyme within the single mitochondrion suggests an important role of the enzyme in kinetoplast DNA replication Crithidia fasciculata ?
-
 ?
5.6.2.2 network of DNA rings + ATP + H2O decatenation Crithidia fasciculata monomeric DNA circles + ADP + phosphate
-
 ?
5.6.2.2 supercoiled DNA + ATP + H2O catenation Crithidia fasciculata catenated DNA networks + ADP + phosphate
-
 ?
5.6.2.2 supercoiled DNA + ATP + H2O relaxation Crithidia fasciculata relaxed DNA + ADP + phosphate
-
 ?
5.6.2.2 supercoiled DNA + ATP + H2O ATP-dependent relaxation Crithidia fasciculata relaxed DNA + ADP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.6.2.2 dimer 2 * 132000, SDS-PAGE Crithidia fasciculata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6.2.2 6.5 8.8
-
 Crithidia fasciculata