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Literature summary extracted from

  • Anselme, J.; Härtlein, M.
    Tyr-426 of the Escherichia coli asparaginyl-tRNA synthetase, an amino acid in a C-terminal conserved motif, is involved in ATP binding (1991), FEBS Lett., 280, 163-166.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.22 Y426F wild-type and mutant enzymes Y426F and Y426S. Y426S replacement leads to a 15fold increase in Km for ATP, while all the other kinetic parameters remain unchanged. Y426F replacement does not affect the kinetic behaviour of the enzyme Escherichia coli
6.1.1.22 Y426S wild-type and mutant enzymes Y426F and Y426S. Y426S replacement leads to a 15fold increase in Km for ATP, while all the other kinetic parameters remain unchanged. Y426F replacement does not affect the kinetic behaviour of the enzyme Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.22 0.0153
-
 Asn wild-type, ATP-diphosphate exchange Escherichia coli
6.1.1.22 0.0225
-
 Asn mutant Y426F, ATP-diphosphate exchange Escherichia coli
6.1.1.22 0.0306
-
 ATP mutant Y426F, aminoacylation Escherichia coli
6.1.1.22 0.0323
-
 Asn wild-type, aminoacylation Escherichia coli
6.1.1.22 0.0326
-
 Asn mutant Y426F, aminoacylation Escherichia coli
6.1.1.22 0.0336
-
 Asn mutant Y426S, aminoacylation Escherichia coli
6.1.1.22 0.0383
-
 Asn mutant Y426S, ATP-diphosphate exchange Escherichia coli
6.1.1.22 0.076
-
 ATP ATP, , wild-type, aminoacylation Escherichia coli
6.1.1.22 0.245
-
 ATP mutant Y426S, aminoacylation Escherichia coli
6.1.1.22 0.5
-
 ATP ATP, , wild-type, mutant Y426F, ATP-diphosphate exchange Escherichia coli
6.1.1.22 7
-
 ATP mutant Y426S, ATP-diphosphate exchange Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.22 Escherichia coli
-
 wild-type and mutant enzymes Y426F and Y426S
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.22 ATP + L-asparagine + tRNAAsn
-
 Escherichia coli AMP + diphosphate + L-asparaginyl-tRNAAsn
-
 ?
6.1.1.22 additional information ATP-diphosphate exchange Escherichia coli ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.22 0.3
-
 ATP mutant Y426S, aminoacylation Escherichia coli
6.1.1.22 0.7
-
 Asn mutant Y426S, aminoacylation Escherichia coli
6.1.1.22 1
-
 Asn ATP, , mutant Y426F, aminoacylation Escherichia coli
6.1.1.22 1.3
-
 ATP Asn, , mutant Y426S, ATP-diphosphate exchange Escherichia coli
6.1.1.22 1.3
-
 ATP wild-type, aminoacylation Escherichia coli
6.1.1.22 1.6
-
 Asn wild-type, aminoacylation Escherichia coli
6.1.1.22 2.1
-
 ATP mutant Y426S, ATP-diphosphate exchange Escherichia coli
6.1.1.22 2.2
-
 ATP wild-type, 37°C, ATP-diphosphate exchange Escherichia coli
6.1.1.22 2.3
-
 ATP mutant Y426F, ATP-diphosphate exchange Escherichia coli
6.1.1.22 2.7
-
 Asn mutant Y426F, , ATP-diphosphate exchange Escherichia coli
6.1.1.22 2.8
-
 Asn mutant Y426F, aminoacylation Escherichia coli
6.1.1.22 2.8
-
 Asn Asn, , wild-type, ATP-diphosphate exchange Escherichia coli