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Literature summary extracted from
- Biosynthesis of monoterpenes: preliminary characterization of bornyl pyrophosphate synthetase from sage (Salvia officinalis) and demonstration that geranyl pyrophosphate is the preferred substrate for cyclization (1979), Arch. Biochem. Biophys., 198, 512-522.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.5.1.8 | diisopropylfluorophosphate | - |
Salvia officinalis |  |
| 5.5.1.8 | p-hydroxymercuribenzoate | - |
Salvia officinalis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.5.1.8 | 0.0014 | - |
geranyl diphosphate | - |
Salvia officinalis | |
| 5.5.1.8 | 0.018 | - |
neryl diphosphate | - |
Salvia officinalis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.5.1.8 | Mg2+ | required | Salvia officinalis | |
| 5.5.1.8 | Mg2+ | Km: 3.5 mM | Salvia officinalis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.5.1.8 | 95000 | - |
gel filtration | Salvia officinalis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.5.1.8 | Salvia officinalis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.5.1.8 | partial | Salvia officinalis |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 5.5.1.8 | geranyl diphosphate = (+)-bornyl diphosphate | mechanism | Salvia officinalis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.5.1.8 | leaf | unfurled leaves from the shoot apex | Salvia officinalis | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.5.1.8 | Geranyl diphosphate | geranyl diphosphate is preferred over neryl diphosphate | Salvia officinalis | (+)-Bornyl-diphosphate | - |
? | |
| 5.5.1.8 | Neryl diphosphate | geranyl diphosphate is preferred over neryl diphosphate | Salvia officinalis | (+)-Bornyl-diphosphate | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.5.1.8 | 6.2 | - |
geranyl diphosphate and neryl diphosphate, the maximal activity with geranyl diphosphate is about 5times higher than with neryl diphosphate | Salvia officinalis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.5.1.8 | 5.7 | 6.7 | pH 5.7: about 40% of maximal activity, pH 6.7: about 35% of maximal activity, geranyl diphosphate | Salvia officinalis |
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