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Literature summary extracted from

  • Inoue, H.; Lowenstein, J.M.
    Acetyl coenzyme A carboxylase from rat liver (1975), Methods Enzymol., 35B, 3-11.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.4.1.2 citrate activates Rattus norvegicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.4.1.2
-
 Rattus norvegicus

General Stability

EC Number General Stability Organism
6.4.1.2 loss of activity after several freeze/thaw-cycles Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.2 long-chain acyl-CoA thioesters
-
 Rattus norvegicus
6.4.1.2 malonyl-CoA
-
 Rattus norvegicus
6.4.1.2 oleoyl-CoA
-
 Rattus norvegicus
6.4.1.2 palmitoyl-CoA
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.2 additional information
-
 additional information
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.2 phosphate contains 9.83 mM of phosphate per mg of protein Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.2 110000
-
 x * 110000, ultracentrifugation in presence of 6 M guanidine hydrochloride and 0.1 M 2-mercaptoethanol Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.2
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.4.1.2 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.2 14.6
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
6.4.1.2 5°C, under N2, 20 mM sodium citrate, 10 mM potassium phosphate buffer, pH 7.5, 1 mM EDTA, 1 mM DTT, protein concentration greater than 2 mg/ml, stable for 1 week Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
 Rattus norvegicus ADP + phosphate + malonyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.4.1.2 ? x * 110000, ultracentrifugation in presence of 6 M guanidine hydrochloride and 0.1 M 2-mercaptoethanol Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.2 7.65
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.4.1.2 7.4 8 about 90% of maximal activity at pH 7.4 and 8.0 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.2 biotin contains 0.91 mol of biotin per dimer of MW 215000 Rattus norvegicus