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Literature summary extracted from

  • Charles, D.J.; Cherry, J.H.
    Purification and characterization of acetyl-CoA carboxylase from developing soybean seeds (1986), Phytochemistry, 25, 1067-1071.
No PubMed abstract available

General Stability

EC Number General Stability Organism
6.4.1.2 DTT, glycerol, Triton X-100, and high concentrations of the enzyme are required for stability Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.2 ADP
-
 Glycine max
6.4.1.2 AMP
-
 Glycine max
6.4.1.2 citrate 10 mM Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.2 0.14
-
 ATP 9686 enzyme Glycine max
6.4.1.2 0.17
-
 acetyl-CoA 9686 enzyme Glycine max
6.4.1.2 0.32
-
 acetyl-CoA Wayne enzyme Glycine max
6.4.1.2 0.46
-
 ATP Wayne enzyme Glycine max
6.4.1.2 1.13
-
 HCO3- Wayne enzyme Glycine max
6.4.1.2 1.56
-
 HCO3- 9686 enzyme Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.2 40000
-
 x * 40000, 9686 enzyme, SDS-PAGE Glycine max
6.4.1.2 58000
-
 x * 58000 + x * 65000 + x * 240000, SDS-PAGE Glycine max
6.4.1.2 65000
-
 x * 58000 + x * 65000 + x * 240000, SDS-PAGE Glycine max
6.4.1.2 240000
-
 x * 58000 + x * 65000 + x * 240000, SDS-PAGE Glycine max

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.2 Glycine max
-
 2 lines of seeds: 9686 and Wayne
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.2
-
 Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.4.1.2 seed 2 lines of seeds: 9686 and Wayne Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.2 additional information
-
-
 Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3- absolute specificity for acetyl-CoA Glycine max ADP + phosphate + malonyl-CoA
-
 ?
6.4.1.2 ATP + butyryl-CoA + HCO3- no activity Glycine max ADP + ethylmalonyl-CoA + phosphate
-
 ?
6.4.1.2 ATP + propionyl-CoA + HCO3- no activity Glycine max ADP + phosphate + methylmalonyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.4.1.2 ? x * 58000 + x * 65000 + x * 240000, SDS-PAGE Glycine max
6.4.1.2 ? x * 40000, 9686 enzyme, SDS-PAGE Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.4.1.2 35 40
-
 Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.2 7.5
-
 9686 enzyme Glycine max
6.4.1.2 8.2
-
 Wayne enzyme Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.2 biotin covalent attachment of the biotin prosthetic group Glycine max