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Literature summary extracted from

  • Murrell, J.C.; Dalton, H.
    Purification and properties of glutamine synthetase from Methylococcus capsulatus (1983), J. Gen. Microbiol., 129, 1187-1196.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.2 Ala
-
 Methylococcus capsulatus
6.3.1.2 AMP
-
 Methylococcus capsulatus
6.3.1.2 Gly
-
 Methylococcus capsulatus
6.3.1.2 His weak Methylococcus capsulatus
6.3.1.2 L-Gln
-
 Methylococcus capsulatus
6.3.1.2 Mg2+ addition of Mg2+ to the Mn2+-dependent transferase activity Methylococcus capsulatus
6.3.1.2 Ser
-
 Methylococcus capsulatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.2 0.15
-
 ADP
-
 Methylococcus capsulatus
6.3.1.2 0.6
-
 ATP
-
 Methylococcus capsulatus
6.3.1.2 0.72
-
 NH4+
-
 Methylococcus capsulatus
6.3.1.2 2.25
-
 Glu
-
 Methylococcus capsulatus
6.3.1.2 14
-
 Glu hydroxylamine, glutamyl transferase assay Methylococcus capsulatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.2 Mg2+ divalent cation required, specificity for Mg2+ in the biosynthetic assay Methylococcus capsulatus
6.3.1.2 Mn2+ supports activity of adenylylated and deadenylylated enzyme, no other divalent cation can support gamma-glutamyl transferase activity, optimal concentration is 0.3 mM Methylococcus capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.2 60000
-
 10 * 60000, SDS-PAGE Methylococcus capsulatus
6.3.1.2 617000
-
 gel filtration Methylococcus capsulatus

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Methylococcus capsulatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.2
-
 Methylococcus capsulatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.2 306
-
-
 Methylococcus capsulatus

Storage Stability

EC Number Storage Stability Organism
6.3.1.2 25 mM imidazole/HCl buffer, pH 7.15, 5 mM MgCl, 10 mM sodium glutamate, 5 mM 2-mercaptoethanol and 1 mM EDTA, at -80°C completely stable for several months, at 4°C stable for several hours Methylococcus capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-Glu + NH4+
-
 Methylococcus capsulatus ADP + phosphate + L-Gln
-
 ?
6.3.1.2 Gln + hydroxylamine + ADP
-
 Methylococcus capsulatus gamma-Glutamylhydroxamate + NH4+ + ?
-
 ?
6.3.1.2 GTP + L-Glu + NH4+ 59% of the activity relative to ATP Methylococcus capsulatus GDP + phosphate + L-Gln
-
 ?
6.3.1.2 ITP + L-Glu + NH4+ 32% of the activity relative to ATP Methylococcus capsulatus IDP + phosphate + L-Gln
-
 ?
6.3.1.2 TTP + L-Glu + NH4+ 10% of the activity relative to ATP Methylococcus capsulatus TDP + phosphate + L-Gln
-
 ?
6.3.1.2 UTP + L-Glu + NH4+ 10% of the activity relative to ATP Methylococcus capsulatus UDP + phosphate + L-Gln
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.1.2 decamer 10 * 60000, SDS-PAGE Methylococcus capsulatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.1.2 45
-
 gamma-glutamyl transferase assay Methylococcus capsulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.2 7.2
-
-
 Methylococcus capsulatus